Differential Expression Patterns of Crystallin Genes during Ocular Development of Olive Flounder (Paralichthys olivaceus)
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Olive flounder Paralichthys olivaceus is one of the most widely cultured fish species in Korea. Although olive flounder receive attention from aquaculture and fisheries and extensive research has been conducted eye morphological change in metamorphosis, but little information was known to molecular mechanism and gene expression of eye development- related genes during the early part of eye formation period. For the reason of eyesight is the most important sense in flounder larvae to search prey, the screening and identification of expressed genes in the eye will provide useful insight into the molecular regulation mechanism of eye development in olive flounder. Through the search of an olive flounder DNA database of expressed sequence tags (EST), we found a partial sequence that was similar to crystallin beta A1 and gamma S. Microscopic observation of retinal formation correspond with the time of expression of the crystallin beta A1 and gamma S gene in the developmental stage, these result suggesting that beta A1 and gamma S play a vital role in the remodeling of the retina during eye development. The expression of crystallin beta A1 and gamma S were obviously strong in eye at all tested developing stage, it is also hypothesized that crystallin acts as a molecular chaperone to prevent protein aggregation during maturation and aging in the eye.
Abstract
Olive flounder Paralichthys olivaceus is one of the most widely cultured fish species in Korea. Although olive flounder receive attention from aquaculture and fisheries and extensive research has been conducted eye morphological change in metamorphosis, but little information was known to molecular mechanism and gene expression of eye development- related genes during the early part of eye formation period. For the reason of eyesight is the most important sense in flounder larvae to search prey, the screening and identification of expressed genes in the eye will provide useful insight into the molecular regulation mechanism of eye development in olive flounder. Through the search of an olive flounder DNA database of expressed sequence tags (EST), we found a partial sequence that was similar to crystallin beta A1 and gamma S. Microscopic observation of retinal formation correspond with the time of expression of the crystallin beta A1 and gamma S gene in the developmental stage, these result suggesting that beta A1 and gamma S play a vital role in the remodeling of the retina during eye development. The expression of crystallin beta A1 and gamma S were obviously strong in eye at all tested developing stage, it is also hypothesized that crystallin acts as a molecular chaperone to prevent protein aggregation during maturation and aging in the eye.
참고문헌 (18)
- Berbers GA, "Primary gene products of bovine betacrystallin and reassociation behavior of its aggregates", Eur J Biochem, 128, pp.495~502 (1982)
- Easter SS, Jr., "The development of vision in the zebrafish", Dev Biol, 180, pp.646~663 (1996)
- Gibb A, "Kinematics of prey capture in a flatfish, Pleuronichthys verticalis", J Exp Biol, 198, pp.1173~1183 (1995)
- Head MW, "Evidence for the extralenticular expression of members of the betacrystallin gene family in the chick and a comparison with delta-crystallin during differentiation and transdifferentiation", Differenti, 48, pp.147~156 (1991)
- Huizinga A, "Local variation in absolute water content of human and rabbit eye lenses measured by Raman microspectroscopy", Exp Eye Res, 48, pp.487~496 (1989)
- 한현자, "Bacterial enteritis and the development of the larval digestive tract in olive flounder, Paralichthys olivaceus (Temminck & Schlegel)", JOURNAL OF FISH DISEASES, 27, pp.497~505, BLACKWELL PUBLISHING
- 이정호, "EST-based Identification of Genes Expressed in the Brain of the Olive Flounder Paralichthys olivaceus", Fisheries and Aquatic Sciences, 12(4), pp.286~292 (2009), 한국수산과학회
- Lee JH, "Molecular characterization of the ocular EST clones from olive flounder", Paralichthys olivaceus Dev Reprod, 14(2), pp.107~113 (2010)
- Martinez GM, "Embryonic and larval staging of summer flounder", J Morphol, 255, pp.162~176 (2003)
- Miwa S, "Effects of various doses of thyroxine and triiodothyronine on the metamorphosis of flounder", Gen Comp Endocrinol, 67, pp.356~363 (1987)
- Miwa S, "Thyroxine surge in metamorphosing flounder larvae", Gen Comp Endocrinol, 70, pp.158~163 (1988)
- Parthasarathy G, "Expression of betaA3", A1-crystallin in the developing and adult rat eye, 1997, pp.42, 59~69
- Philipson B, "Distribution of protein within the normal rat lens", Invest Ophthalmol, 8, pp.258~270 (1969)
- Ray ME, "AIM1, a novel non-lens member of the betagammacrystallin superfamily, is associated with the control of tumorigenicity in human malignant melanoma", Proc Natl Acad Sci USA, 94, pp.3229~3234 (1997)
- Sax CM, "Expression of the alphacrystallin", small heat-shock protein, Adv Enzymol Relat Areas Mol Biol, pp.1994, 69[DOI]
- Shand J, "Optics of the developing fish eye: comparisons of Matthiessen's ratio and the focal length of the lens in the black bream Acanthopagrus butcheri", Vision Res, 39, pp.1071~1078 (1999)
- Wistow G, "Lens crystallins: gene recruitment and evolutionary dynamism", Trends Biochem Sci, 18, pp.301~306 (1993)
- Wistow GJ, "Lens crystallins: the evolution and expression of proteins for a highly specialized tissue", Annu Rev Biochem, 57, pp.479~504 (1988)