Decreased Expression of Inhibitor of DNA-binding (Id) Proteins and Vascular Endothelial Growth Factor and Increased Apoptosis in Ovarian Aging
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This study examined the expression of inhibitor of DNA-binding (Id) proteins and vascular endothelial growth factor (VEGF) in the ovary according to female age using a mice model as the first step in investigating the potential role of Ids and VEGF in ovarian aging. C57BL inbred female mice of three age groups (6－9, 14－16, and 23－26weeks) were injected with 5 IU pregnant mare’s serum gonadotropin (PMSG) in order to synchronize the estrus cycle. After 48 h, ovarian expression of Ids and VEGF was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR),western blot and immunohistochemistry. Ovarian apoptosis was examined by ovarian expression of Bcl-2 and Bcl-xL. Expression of Id-1 and VEGF was decreased with advancing female age, but not Id-2, Id-3, and Id-4. In particular, their expressions were significantly decreased in aged mice of 23－26 weeks compared with the young mice of 6－9 weeks (p < 0.05). In contrast, ovarian apoptosis was greatly increased in the aged mice compared to the young mice. This result suggests that Id-1 may have an implicated role in ovarian aging by associating with VEGF.
Abstract
This study examined the expression of inhibitor of DNA-binding (Id) proteins and vascular endothelial growth factor (VEGF) in the ovary according to female age using a mice model as the first step in investigating the potential role of Ids and VEGF in ovarian aging. C57BL inbred female mice of three age groups (6－9, 14－16, and 23－26weeks) were injected with 5 IU pregnant mare’s serum gonadotropin (PMSG) in order to synchronize the estrus cycle. After 48 h, ovarian expression of Ids and VEGF was analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR),western blot and immunohistochemistry. Ovarian apoptosis was examined by ovarian expression of Bcl-2 and Bcl-xL. Expression of Id-1 and VEGF was decreased with advancing female age, but not Id-2, Id-3, and Id-4. In particular, their expressions were significantly decreased in aged mice of 23－26 weeks compared with the young mice of 6－9 weeks (p < 0.05). In contrast, ovarian apoptosis was greatly increased in the aged mice compared to the young mice. This result suggests that Id-1 may have an implicated role in ovarian aging by associating with VEGF.
참고문헌 (27)
- Alani RM, "Immortalization of primary human keratinocytes by the helix-loop-helix protein, Id-1", Proc Natl Acad USA, 96, pp.9637~9641 (1999)
- Danforth DR, "Vascular endothelial growth factor stimulates preantral follicle growth in the rat ovary", Biol Reprod, 68, pp.1736~1741 (2003)
- Fraser HM, "Regulation of the ovarian follicular vasculature", Reprod Biol Endocrinol, 4, pp.18~26 (2006)
- Geva E, "Role of vascular endothelial growth factor in ovarian physiology and pathology", Fertil Steril, 74, pp.429~438 (2000)
- Ha CS, "Estrogen administration during superovulation increases oocyte quality and expressions of vascular endothelial growth factor and nitric oxide synthase in the ovary", JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH, 36(4), pp.789~795 (2010), WILEY-BLACKWELL PUBLISHING
- Hogg K, "Inhibitor of differentiation (Id) genes are expressed in the steroidogenic cells of the ovine ovary and are differentially regulated by members of the transforming growth factor-beta family", Endocrinology, 151, pp.1247~1256 (2010)
- Iijima K, "Acceleration of follicular development by administration of vascular endothelial growth factor in cycling female rats", J Reprod Dev, 51, pp.161~168 (2005)
- Johnson AL, "Role for inhibitor of differentiation", deoxyribonucleic acid-binding (Id) proteins in granulosa cell differentiation, 2008, pp.149, 3187~3195
- Johnson AL, "Dynamics of avian ovarian follicle development: cellular mechanisms of granulosa cell differentiation", General and Comparative Endocrinology, 163, pp.12~17 (2009)
- Joo JK, "Role of leptin in improvement of oocyte quality by regulation of ovarian angiogenesis", Anim Reprod Sci, 119, pp.329~334 (2010)
- Kaczmarek MM, "Role of vascular endothelial growth factor in ovarian physiologyan overview", Reprod Biol, 5, pp.111~136 (2005)
- Keefe DL, "Mitochondrial deoxyribonucleic acid deletions in oocytes and reproductive aging in women", Fertil Steril, 64, pp.577~583 (1995)
- Kim HJ, "Inhibitor of DNA binding 1 activates vascular endothelial growth factor through enhancing the stability and activity of hypoxia-inducible factor-1alpha", Mol Cancer Res, 5, pp.321~329 (2007)
- Korchynskyi O, "Identification and functional characterization of distinct critically important bone morphogenetic protein-specific response elements in the Id1 promoter", J Biol Chem, 277, pp.4883~4891 (2002)
- Lee DH, "Sodium nitroprusside treatment during the superovulation process improves ovarian response and ovarian expression of vascular endothelial growth factor in aged female mice", Fertil Steril, 89(Suppl), pp.1514~1521 (2008)
- Ling MT, "Wong YC(2005)Overexpression of Id-1 in prostate cancer cells promotes angiogenesis through the activation of vascular endothelial growth factor(VEGF)", Carcinogenesis, 26, pp.1668~1676 (2005)
- Maw MK, "Tamaya T(2008)Expression of the inhibitor of DNA-binding(ID)-1 protein as an angiogenic mediator in tumor advancement of uterine cervical cancers", Br J Cancer, 99, pp.1557~1563 (2008)
- Miyoshi T, "Makino H(2007)Differential regulation of steroidogenesis by bone morphogenetic proteins in granulosa cells : involvement of extracellularly regulated kinase signaling and oocyte actions in folliclestimulating hormone-induced estrogen production", Endocrinology, 148, pp.337~345 (2007)
- Norton JD, "Atherton GT(1998)Coupling of cell growth control and apoptosis functions of Id proteins", Mol Cell Biol, 18, pp.2371~2381 (1998)
- Norton JD, "Sablitzky F(1998)Id helix-loop-helix proteins in cell growth and differentiation", Trends Cell Biol, 8, pp.58~65 (1998)
- Shimasaki S, "A functional bone morphogenetic protein system in the ovary", Proc Natl Acad Sci USA, 96, pp.7282~7287 (1999)
- Shimizu T, "Sato E(2003)Induction of follicular development by direct single injection of vascular endothelial growth factor gene fragments into the ovary of miniature glits", Biol Reprod, 69, pp.1388~1393 (2003)
- Tarin JJ, "Cano A(2001)Cellular and morphological traits of oocytes retrieved from aging mice after exogenous ovarian stimulation", Biol Reprod, 65, pp.141~150 (2001)
- Tatone C, "Focarelli R(2008)Cellular and molecular aspects of ovarian follicle ageing", Hum Reprod Update, 14, pp.131~142 (2008)
- te Velde ER, "Peasron PL(2002)The variability of female reproductive aging", Hum Reprod Update, 8, pp.141~154 (2002)
- Thouas GA, "Jones GM(2005)Effect of female age on mouse oocyte developmental competence following mitochondrial injury", Biol Reprod, 73, pp.366~373 (2005)
- Wulff C, "and ovulation in primate by treatment with vascular endothelial growth factor trap R1R2", Endocrinology, 43, pp.2797~2807 (2002)