Genetic Distances of Three White Clam (Meretrix lusoria) Populations Investigated by PCR Analysis
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The twenty-one individuals of Meretrix lusoria were secured from Gunsan, Shinan and Yeonggwang on thecoast of the Yellow Sea and the southern sea in the Korean Peninsula, respectively. Amplification of a single COI fragment(720 bp) was imagined, and no apparent size differences were observed in amplified fragments between Meretrix lusoria andM. petechialis individuals. The size of the DNA fragments also varied excitedly, from 200 to 1,600 bp. The oligonucleotidesprimer BION-08 produced the least loci (a total of 17), with an average of 2.43 in the Gunsan population, in comparison to theother primers used. Remarkably, the primer BION-13 detected 42 shared loci by the three populations, major and/or minorfragments of sizes 200 bp and 400 bp, respectively, which were identical in all samples. The dendrogram gained by the sevenoligonucleotides primers highlight three genetic clusters: cluster 1 (GUNSAN 01 ~ GUNSAN 07), cluster 2 (SHINAN 08 ~SHINAN 14) and cluster 3 (YEONGGWANG 15 ~ YEONGGWANG 21). The longest genetic distance among the twentyoneMeretrix lusoria individuals that displayed significant molecular differences was between individuals GUNSAN no. 01and SHINAN no. 14 (genetic distance = 0.574). Comparatively, individuals of SHINAN population were fairly closely relatedto that of YEONGGWANG population. In this study, PCR analysis has discovered significant genetic distances between twowhite clam population pairs (P<0.05).
Abstract
The twenty-one individuals of Meretrix lusoria were secured from Gunsan, Shinan and Yeonggwang on thecoast of the Yellow Sea and the southern sea in the Korean Peninsula, respectively. Amplification of a single COI fragment(720 bp) was imagined, and no apparent size differences were observed in amplified fragments between Meretrix lusoria andM. petechialis individuals. The size of the DNA fragments also varied excitedly, from 200 to 1,600 bp. The oligonucleotidesprimer BION-08 produced the least loci (a total of 17), with an average of 2.43 in the Gunsan population, in comparison to theother primers used. Remarkably, the primer BION-13 detected 42 shared loci by the three populations, major and/or minorfragments of sizes 200 bp and 400 bp, respectively, which were identical in all samples. The dendrogram gained by the sevenoligonucleotides primers highlight three genetic clusters: cluster 1 (GUNSAN 01 ~ GUNSAN 07), cluster 2 (SHINAN 08 ~SHINAN 14) and cluster 3 (YEONGGWANG 15 ~ YEONGGWANG 21). The longest genetic distance among the twentyoneMeretrix lusoria individuals that displayed significant molecular differences was between individuals GUNSAN no. 01and SHINAN no. 14 (genetic distance = 0.574). Comparatively, individuals of SHINAN population were fairly closely relatedto that of YEONGGWANG population. In this study, PCR analysis has discovered significant genetic distances between twowhite clam population pairs (P<0.05).
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