Expression Analysis of Lily Type Lectin Isotypes in the Rock Bream, Oplegnathus fasciatus: in the Tissue, Developmental Stage and Viral Infection
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Lectins belong to the pattern-recognition receptors (PRRs) class and play important roles in the recognition and elimination of pathogens via the innate immune system. Recently, it was reported that lily-type lectin-1 is involved when a pathogen attacks in the early immune response of fish. However, this study is limited to information that the lectin is involved in the innate immune response against viral infection. In the present study, the lily-type lectin-2 and -3 of Oplegnathus fasciatus (OfLTL-2 and 3) have been presented to be included B-lectin domain and two D-mannose binding sites in the amino acid sequence that an important feature for the fundamental structure. To investigate the functional properties of OfLTLs, the tissue distribution in the healthy rock bream and temporal expression during early developmental stage analysis are performed using quantitative real-time PCR. OfLTL-2 and 3 are predominantly expressed in the liver and skin, but rarely expressed in other organ. Also, the transcripts of OfLTLs are not expressed during the early developmental stage but its transcripts are increased after immune-related organs which are fully formed. In the challenge experiment with RBIV (rock bream iridovirus), the expression of OfLTLs was increased much more strongly in the late response than the early, unlike previously known. These results suggest that OfLTLs are specifically expressed in the immune-related tissues when those organs are fully formed and it can be inferred that the more intensively involved in the second half to the virus infection.
Abstract
Lectins belong to the pattern-recognition receptors (PRRs) class and play important roles in the recognition and elimination of pathogens via the innate immune system. Recently, it was reported that lily-type lectin-1 is involved when a pathogen attacks in the early immune response of fish. However, this study is limited to information that the lectin is involved in the innate immune response against viral infection. In the present study, the lily-type lectin-2 and -3 of Oplegnathus fasciatus (OfLTL-2 and 3) have been presented to be included B-lectin domain and two D-mannose binding sites in the amino acid sequence that an important feature for the fundamental structure. To investigate the functional properties of OfLTLs, the tissue distribution in the healthy rock bream and temporal expression during early developmental stage analysis are performed using quantitative real-time PCR. OfLTL-2 and 3 are predominantly expressed in the liver and skin, but rarely expressed in other organ. Also, the transcripts of OfLTLs are not expressed during the early developmental stage but its transcripts are increased after immune-related organs which are fully formed. In the challenge experiment with RBIV (rock bream iridovirus), the expression of OfLTLs was increased much more strongly in the late response than the early, unlike previously known. These results suggest that OfLTLs are specifically expressed in the immune-related tissues when those organs are fully formed and it can be inferred that the more intensively involved in the second half to the virus infection.
참고문헌 (22)
- Arasu A, "Fish lily type lectin-1 contains β-prism architecture: Immunological characterization", Mol Immunol, 56, pp.497~506 (2013)
- Castro R, "Early immune responses in rainbow trout liver upon viral hemorrhagic septicemia virus (VHSV) infection", PLoS One, 22, pp.e111084~ (2014)
- Choi KM, "Functional characterisation and expression analysis of recombinant serum amyloid P isoform 1 (RbSAP1) from rock bream (Oplegnathus fasciatus)", Fish Shellfish Immunol, 45, pp.277~285 (2015)
- Inouye K, "Iridovirus infection of cultured red sea bream, Pagrus major", Fish Pathol, 27, pp.19~27 (1992)
- Kim BS, "Molecular characterisation and expression analysis of a fish-egg lectin in rock bream, and its response to bacterial or viral infection", Fish Shellfish Immunol, 31, pp.1201~1207 (2011)
- Kim JW, "Molecular characterisation and expression analysis of the cathepsin H gene from rock bream (Oplegnathus fasciatus)", Fish Shellfish Immunol, 35, pp.188~194 (2013)
- 이정호, "Expression of Immune-Related Genes during Loach (Misgurnus anguillicaudatus) Embryonic and Early Larval Development", 발생과 생식, 19(4), pp.181~187 (2015), 한국발생생물학회
- Magnadóttir B, "Innate immunity of fish (overview)", Fish Shellfish Immunol, 20, pp.137~151 (2006)
- Nakajima K, "Biological and physicochemical properties of the iridovirus isolated from cultured red sea bream, Pagrus major", Fish Pathol, 29, pp.29~33 (1994)
- Park HJ, "Molecular cloning and expression analysis of two distinct F-type lectins from the rock bream, Oplegnathus fasciatus", Dev Comp Immunol, 36, pp.230~235 (2012)
- Park HJ, "Molecular cloning and expression analysis of a new lily-type lectin in the rock bream, Oplegnathus fasciatus", Dev Comp Immunol, 65, pp.25~30 (2016)
- Pfaffl MW, "A new mathematical model for relative quantification in real-time RT-PCR", Nucleic Acids Res, 29, pp.e45~ (2001)
- Racanelli V, "The liver as an immunological organ", Hepatology, 43, pp.54~62 (2006)
- Saraiva TC, "Nattectin a fish C-type lectin drives Th1 responses in vivo: licenses macrophages to differrentiate into cells exhibiting typical DC function", Int Immunopharmacol, 11, pp.1546~1556 (2011)
- Sharon N, "History of lectins: from hemagglutinins to biological recognition molecules", Glycobiology, 4, pp.53~62 (2004)
- Suzuki Y, "Molecular diversity of skin mucus lectins in fish", Comp Biochem Physiol B Biochem Mol Biol, 136, pp.723~730 (2003)
- Tasumi S, "Identification and characterization of pufflectin from the grass pufferfish Takifugu niphobles and comparison of its expression with that of Takifugu rubripes", Dev Comp Immunol, 59, pp.48~56 (2016)
- Thulasitha WS, "Antimicrobial response of galectin-1 from rock bream Oplegnathus fasciatus: Molecular, transcripttional, and biological characterization", Fish Shellfish Immunol, 50, pp.66~78 (2016)
- Umasuthan N, "A manganese superoxide dismutase with potent antioxidant activity identified from Oplegnathus fasciatus: genomic structure and transcriptional characterrization", Fish Shellfish Immunol, 34, pp.23~37 (2013)
- Wassaman PM, "Nature of the mouse egg’s receptor for sperm", Adv Exp Med Biol, 207, pp.55~77 (1986)
- Wilkins MR, "Protein identification and analysis tools in the ExPASy server", Methods Mol Biol, 112, pp.531~552 (1999)
- Zhizhong X, "Ontogeny of the immune system in rock bream Oplegnathus fasciatus", Chin J Oceanol Limnol, 31, pp.1028~1035 (2013)