Starvation-induced Physiological Responses and RNA/DNA Ratios in Rock Bream, Oplegnathus fasciatus, and Olive Flounder, Paralichthys olivaceus
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
In a 12-week experiment, the rock bream, Oplegnathus fasciatus, and olive flounder, Paralichthys olivaceus, were investigated to determine the effects of starvation on their physiological parameters. The protein and DNA contents of the starved fish were significantly higher than the initial values and those of the fed fish. The RNA contents and RNA/DNA ratios of the fed fish were significantly higher than those of the other groups (P<0.05). The hematocrit, hemoglobin, red blood cells (RBC), and mean corpuscular volume (MCV) of the fed rock bream were significantly higher than at baseline (P<0.05), whereas the mean corpuscular hemoglobin concentration (MCHC) of the fed fish was lower than at baseline (P<0.05). The hematocrit, hemoglobin, RBC, and MCHC of the starved group were significantly lower than the baseline values, whereas the MCV of the starved group was significantly higher than the baseline value (P<0.05). No significant difference in alanine aminotransferase was observed between the fed fish and baseline, whereas the starved fish value was significantly higher than the baseline value (P>0.05). There were no significant differences in cortisol levels. However, the glucose level in the fed group was significantly higher than the baseline level and that in the starved group was significantly lower than the baseline level (P<0.05).
Abstract
In a 12-week experiment, the rock bream, Oplegnathus fasciatus, and olive flounder, Paralichthys olivaceus, were investigated to determine the effects of starvation on their physiological parameters. The protein and DNA contents of the starved fish were significantly higher than the initial values and those of the fed fish. The RNA contents and RNA/DNA ratios of the fed fish were significantly higher than those of the other groups (P<0.05). The hematocrit, hemoglobin, red blood cells (RBC), and mean corpuscular volume (MCV) of the fed rock bream were significantly higher than at baseline (P<0.05), whereas the mean corpuscular hemoglobin concentration (MCHC) of the fed fish was lower than at baseline (P<0.05). The hematocrit, hemoglobin, RBC, and MCHC of the starved group were significantly lower than the baseline values, whereas the MCV of the starved group was significantly higher than the baseline value (P<0.05). No significant difference in alanine aminotransferase was observed between the fed fish and baseline, whereas the starved fish value was significantly higher than the baseline value (P>0.05). There were no significant differences in cortisol levels. However, the glucose level in the fed group was significantly higher than the baseline level and that in the starved group was significantly lower than the baseline level (P<0.05).
참고문헌 (32)
- An HS, "Microsatellite DNA loci in the rock bream Oplegnathus fasciatus", Mole Ecol, 6, pp.44~46 (2006)
- Barton BA, "Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids", Ann Rev Fish Dis, 1, pp.3~26 (1991)
- Barton BA, "Metabolic cost of acute physical stress in juvenile steel-head", Trans Amer Fisher Soc, 116, pp.257~263 (1987)
- Barton BA, "Effects of chronic cortisol administration and daily acute stress on growth, physiological conditions and stress responses in juvenile rainbow trout", Dis Aquat Organisms, 2, pp.173~185 (1987)
- Brachet J, "The Nucleic Acids, Chemistry and Biology", pp.576~ (1955), Academic Press
- Brouche G, "Fasting and renutrition of carp, Cyprinus carpio (L.), I. Influence on the nucleic acids of liver", Archives des Sciences Physiologiques, 24, pp.243~251 (1970)
- Buckely LJ, "Biochemical changes during ontogenesis of cod, Gadus morhua, and winter flounder, Pseudopleuronectes americanus, larvae. Rapports et Procesverbaux des Réunions", Conseil International pour l'Éxploration de la Mer, 178, pp.547~552 (1981)
- Buckley LJ, "Relationshop between RNA", DNA ratio, prey density and growth rate in Atlantic cod, Gadus morhua, larvae, 1979, pp.36, 1497~1502
- Buckley LJ, "Changes in ribonucleic acid, deoxyribonucleic acid and protein content during ontogenesis in winter flounder, Pseudopleuronectes americanus, and the effect of starvation", Fish Bull, 77, pp.703~708 (1980)
- Bulow FJ, "RNA", DNA ratios as indicators of recent growth rates of a fish, 1970, pp.27, 2343~2349
- Carmichael GJ, "Confinement and water quality-induced stress in largemouth bass", Trans Amer Fisher Soc, 113, pp.767~777 (1984)
- Carmichael GJ, "Characterization and alleviation of stress associated with hauling largemouth bass", Trans Amer Fisher Soc, 113, pp.778~785 (1984)
- Davis KB, "Biotic and abiotic influences on corticosteroid hormone rhythms in channel catfish", Trans Amer Fisher Soc, 113, pp.414~421 (1984)
- Davis KB, "Growth, body composition and hepatic tyrosine aminotransferase activity in cortisol-fed channel catfish, Ictalurus punctatus Rafinesque", J Fish Biol, 27, pp.177~184 (1985)
- Donaldson EM, "Stress in Fish", pp.11~47 (1981), Academic Press
- Dortch Q, "RNA", DNA rations and DNA concentrations as indicators of growth rage and biomass in planktonic marine organisms, 1983, pp.13, 61~71
- Haines TA, "An evalution of RNA-DNA ratio as a measure of long-term growth in fish populations", J Fisher Res Board Can, 30, pp.195~199 (1973)
- Iain C, "Starvation, exercise, injury and obesity", Anaesth Intens Care Medi, 8, pp.299~303 (2007)
- Ince BW, "The effects of starvation and force-feeding on the metabolism of the northern pick, Esox lucius (L.)", J Fish Biol, 8, pp.79~88 (1976)
- Larsson A, "Metabolic effects of starvation in the eel, Anguilla anguilla L", Comp Biochem Physiol, 44, pp.367~374 (1973)
- Lee K-K, "Effect of starvation on some nutritional parameters in Rhynchocypris oxycephalus. 1. Characterictics of the histological and biochemical changes", Kor J Ichthyol, 11, pp.33~41 (1999)
- Love RM, "Depletion", pp.222~257 (1970), Academic Press
- Love RM, "Feeding and Starvation", pp.133~229 (1968), Academic Press
- Lowery OH, "Protein measurement with frolin phenol reagent", J Biol Chem, 130, pp.265~275 (1951)
- Mustafa S, "Condition indices and RNA : DNA ratio in overwintering European sea bass, Dicentrarchus labrax, in salt marshes along the Atlantic coast of France", Aquaculture, 96, pp.367~374 (1991)
- Ohnishi ST, "A simplified method of quantitiating protein using the buiret and phenol reagents", Anal Biochem, 86, pp.193~ (1978)
- Park I-S, "Genetic characterization, morphometric and gonad development of induced hybrids between yellowtail flounder, Pleuronectes ferrugineus (Storer) and winter flounder, Pleuronectes americanus (Walbaum)", Aquacult Res, 34, pp.389~396 (2003)
- 박인석, "넙치의 사료공급과 절식시 성장과 표현형질 특징", 한국양식학회지, 19(3), pp.183~187 (2006), 한국양식학회
- Regnault M, "Study by evolution of nucleic acid content of prepuberal growth in the shrimp, Crangon vulgaris", Mar Biol, 25, pp.291~298 (1974)
- Ruane NM, "Differences between rainbow trout and brown trout in the regulation of the pituitary-interrenal axis and physiological performance during confinement", Gen Comp Endocrin, 115, pp.210~219 (1999)
- Schneider WC, "Determination of nucleic acids in tissues by pentose analysis", Methods in Enzymology, 3, pp.1154~ (1957), Academic Press
- Weatherley AH, "Protein, Lipid and Caloric", pp.139~146 (1987), Academic Press