Proline-Rich Acidic Protein 1 (PRAP1) is a Target of ARID1A and PGR in the Murine Uterus
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ARID1A and PGR plays an important role in embryo implantation and decidualization during early pregnancy. Uterine specific Arid1a knockout (Pgrcre/+Arid1a f/f) mice exhibit in non-receptive endometrium at day 3.5 of gestation (GD 3.5). In previous studies, using transcriptomic analysis in the uterus of Pgrcre/+Arid1a f/f mice, we identified proline-rich acidic protein 1 (PRAP1) as one of the down-regulated genes by ARID1A in the uterus. In the present study, we performed RTqPCR and immunohistochemistry analysis to investigate the regulation of PRAP1 by ARID1A and determine expression patterns of PRAP1 in the uterus during early pregnancy. During early pregnancy, PRAP1 expression was strong at day 0.5 of gestation (GD 0.5) and then decreased at GD 3.5 in the epithelium and stroma. After implantation, PRAP1 expression was remarkably reduced in the uterus. However, the expression of PRAP1 at GD 3.5 was remarkably increased in the Pgrcre/+Arid1a f/f mice. To determine the ovarian steroid hormone regulation of PRAP1, we examined the expression of PRAP1 in ovariectomized control, Pgrcre/+Arid1a f/f, and progesterone receptor knock-out (PRKO) mice treated with progesterone. While PRAP1 proteins were strongly expressed in the luminal and glandular epithelium of control mice treated with vehicle, progesterone treatment suppressed the expression of PRAP1. However, PRAP1 was not suppressed in both the Pgrcre/+Arid1a f/f and PRKO mice compared to controls. Our results identified PRAP1 as a novel target of ARID1A and PGR in the murine uterus.
Abstract
ARID1A and PGR plays an important role in embryo implantation and decidualization during early pregnancy. Uterine specific Arid1a knockout (Pgrcre/+Arid1a f/f) mice exhibit in non-receptive endometrium at day 3.5 of gestation (GD 3.5). In previous studies, using transcriptomic analysis in the uterus of Pgrcre/+Arid1a f/f mice, we identified proline-rich acidic protein 1 (PRAP1) as one of the down-regulated genes by ARID1A in the uterus. In the present study, we performed RTqPCR and immunohistochemistry analysis to investigate the regulation of PRAP1 by ARID1A and determine expression patterns of PRAP1 in the uterus during early pregnancy. During early pregnancy, PRAP1 expression was strong at day 0.5 of gestation (GD 0.5) and then decreased at GD 3.5 in the epithelium and stroma. After implantation, PRAP1 expression was remarkably reduced in the uterus. However, the expression of PRAP1 at GD 3.5 was remarkably increased in the Pgrcre/+Arid1a f/f mice. To determine the ovarian steroid hormone regulation of PRAP1, we examined the expression of PRAP1 in ovariectomized control, Pgrcre/+Arid1a f/f, and progesterone receptor knock-out (PRKO) mice treated with progesterone. While PRAP1 proteins were strongly expressed in the luminal and glandular epithelium of control mice treated with vehicle, progesterone treatment suppressed the expression of PRAP1. However, PRAP1 was not suppressed in both the Pgrcre/+Arid1a f/f and PRKO mice compared to controls. Our results identified PRAP1 as a novel target of ARID1A and PGR in the murine uterus.
참고문헌 (37)
- Adams NR, "The role of steroid hormone receptors in the establishment of pregnancy in rodents", Adv Anat Embryol Cell Biol, 216, pp.27~49 (2015)
- Bhurke AS, "Progesterone-regulated endometrial factors controlling implantation", Am J Reprod Immunol, 75, pp.237~245 (2016)
- Chunder N, "Deletion mapping of chromosome 1 in early onset and late onset breast tumors: A comparative study in eastern India", Pathol Res Pract, 199, pp.313~321 (2003)
- Czyzyk A, "The role of progesterone therapy in early pregnancy: From physiological role to therapeutic utility", Gynecol Endocrinol, 33, pp.421~424 (2017)
- Das SK, "Regional development of uterine decidualization: Molecular signaling by Hoxa-10", Mol Reprod Dev, 77, pp.387~396 (2010)
- Diao H, "Uterine luminal epithelium-specific proline-rich acidic protein 1 (PRAP1) as a marker for successful embryo implantation", Fertil Steril, 94, pp.2808~2811 (2010)
- Fox C, "Local and systemic factors and implantation: What is the evidence?", Fertil Steril, 105, pp.873~884 (2016)
- Franco HL, "Epithelial progesterone receptor exhibits pleiotropic roles in uterine development and function", FASEB J, 26, pp.1218~1227 (2012)
- Grandi G, "The association between progestins, nuclear receptors expression and inflammation in endometrial stromal cells from women with endometriosis", Gynecol Endocrinol, 33, pp.712~715 (2017)
- Guan B, "Mutation and loss of expression of ARID1A in uterine low-grade endometrioid carcinoma", Am J Surg Pathol, 35, pp.625~632 (2011)
- Guan B, "ARID1A, a factor that promotes formation of SWI", SNF-mediated chromatin remodeling, is a tumor suppressor in gynecologic cancers, 2011, pp.71, 6718~6727
- Guo X, "ARID1A and CEBPalpha cooperatively inhibit UCA1 transcription in breast cancer", Oncogene, 37, pp.5939~5951 (2018)
- Han SJ, "The dynamics of nuclear receptors and nuclear receptor coregulators in the pathogenesis of endometriosis", Human Reprod Update, 20, pp.467~484 (2014)
- Huang BH, "PRAP1 is a novel executor of p53-dependent mechanisms in cell survival after DNA damage", Cell Death Dis, 3, pp.e442~ (2012)
- Huet-Hudson YM, "Cell typespecific localization of c-myc protein in the mouse uterus: Modulation by steroid hormones and analysis of the periimplantation period", Endocrinology, 125, pp.1683~1690 (1989)
- Islam MS, "Extracellular matrix in uterine leiomyoma pathogenesis: A potential target for future therapeutics", Human Reprod Update, 24, pp.59~85 (2017)
- Jones S, "Frequent mutations of chromatin remodeling gene ARID1A in ovarian clear cell carcinoma", Science, 330, pp.228~231 (2010)
- Kasik J, "A novel complementary deoxyribonucleic acid is abundantly and specifically expressed in the uterus during pregnancy", Am J Obstet Gynecol, 176, pp.452~456 (1997)
- Kim TH, "ARID1A is essential for endometrial function during early pregnancy", PLOS Genet, 11, pp.e1005537~ (2015)
- Lanemo Myhrinder A, "A new perspective: Molecular motifs on oxidized LDL, apoptotic cells, and bacteria are targets for chronic lymphocytic leukemia antibodies", Blood, 111, pp.3838~3848 (2008)
- Lee K, "Molecular mechanisms involved in progesterone receptor regulation of uterine function", J Steroid Biochem Mol Biol, 102, pp.41~50 (2006)
- Lydon JP, "Mice lacking progesterone receptor exhibit pleiotropic reproductive abnormalities", Genes Dev, 9, pp.2266~2278 (1995)
- Tsui-Lien Mao, "The roles of ARID1A in gynecologic cancer", Journal of Gynecologic Oncology, 24(4), pp.376~381 (2013), 대한부인종양학회
- Marquardt RM, "Progesterone and estrogen signaling in the endometrium: What goes wrong in endometriosis?", Int J Mol Sci, 20, pp.3822~ (2019)
- Patel BG, "Progesterone resistance in endometriosis: Origins, consequences and interventions", Acta Obstet Gynecol Scand, 96, pp.623~632 (2017)
- Prior JC, "Progesterone for symptomatic perimenopause treatment: Progesterone politics, physiology and potential for perimenopause", Facts Views Vis Obgyn, 3, pp.109~120 (2011)
- Samartzis EP, "ARID1A mutations and PI3K", AKT pathway alterations in endometriosis and endometriosis-associated ovarian carcinomas, 2013, pp.14, 18824~18849
- Sze KM, "Proline-rich acidic protein 1 (PRAP1) is a novel interacting partner of MAD1 and has a suppressive role in mitotic checkpoint signalling in hepatocellular carcinoma", J Pathol, 233, pp.51~60 (2014)
- Tabibzadeh S, "Molecular control of the implantation window", Hum Reprod Update, 4, pp.465~471 (1998)
- Takeda T, "ARID1A gene mutation in ovarian and endometrial cancers (Review)", Oncol Rep, 35, pp.607~613 (2016)
- Teasley HE, "Expression of PIK3IP1 in the murine uterus during early pregnancy", Biochem Biophys Res Commun, 495, pp.2553~2558 (2018)
- Vasquez YM, "Role of nuclear receptors in blastocyst implantation", Semin Cell Dev Biol, 24, pp.724~735 (2013)
- Wetendorf M, "Progesterone receptor signaling in the initiation of pregnancy and preservation of a healthy uterus", Int J Dev Biol, 58, pp.95~106 (2014)
- Wu JN, "ARID1A mutations in cancer: Another epigenetic tumor suppressor?", Cancer Discov, 3, pp.35~43 (2013)
- Xiong GF, "Estradiol-regulated proline-rich acid protein 1 is repressed by class I histone deacetylase and functions in peri-implantation mouse uterus", Mol Cell Endocrinol, 331, pp.23~33 (2011)
- Yoo JY, "MIG-6 negatively regulates STAT3 phosphorylation in uterine epithelial cells", Oncogene, 37, pp.255~262 (2017)
- Zhang J, "The proline-rich acidic protein is epigenetically regulated and inhibits growth of cancer cell lines", Cancer Res, 63, pp.6658~6665 (2003)