Fish Myogenic Regulatory Protein LUC7L: Characterization and Expression Analysis in Korean Rose Bitterling (Rhodeus uyekii)
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초록
Serine-arginine-rich nuclear protein LUC7L plays an important role in the regulation of myogenesis in mice. In the present study, we isolated and characterized the Korean rose bitterling Rhodeus uyekii Luc7l cDNA, designatedRuLuc7l. The RuLuc7l cDNA is 1,688 bp long and encodes a 364-amino-acid polypeptide containing serine/arginine-richregion at the C-terminus. The deduced RuLuc7l protein has high amino acid identity (71-97%) with those of other speciesincluding human. Phylogenetic analysis revealed that RuLUC7L clustered with fish LUC7L proteins. The expression ofRuLuc7l mRNA was high in the brain, kidney, and stomach of Korean rose bitterling. Expression of the RuLuc7l mRNA wasdetected from 1 day post-fertilization (dpf) and moderately increased until 21 dpf during the early development. Furtherinvestigations are required to elucidate the functional role of RuLUC7L in myogenesis in R. uyekii.
Abstract
Serine-arginine-rich nuclear protein LUC7L plays an important role in the regulation of myogenesis in mice. In the present study, we isolated and characterized the Korean rose bitterling Rhodeus uyekii Luc7l cDNA, designatedRuLuc7l. The RuLuc7l cDNA is 1,688 bp long and encodes a 364-amino-acid polypeptide containing serine/arginine-richregion at the C-terminus. The deduced RuLuc7l protein has high amino acid identity (71-97%) with those of other speciesincluding human. Phylogenetic analysis revealed that RuLUC7L clustered with fish LUC7L proteins. The expression ofRuLuc7l mRNA was high in the brain, kidney, and stomach of Korean rose bitterling. Expression of the RuLuc7l mRNA wasdetected from 1 day post-fertilization (dpf) and moderately increased until 21 dpf during the early development. Furtherinvestigations are required to elucidate the functional role of RuLUC7L in myogenesis in R. uyekii.
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