Comparison of Cell and Nuclear Size Difference between Diploid and Induced Triploid in Marine Medaka, Oryzias dancena
1
조회
0
다운로드
9
인용
0
참고문헌
키워드
초록
The influence of triploidization on cell and nucleus size characteristics of the same tissues of erythrocyte, retina, kidney, hepatocyte and midgut epithelium in marine medaka, Oryzias dancena has been determined histologically. Induced triploid fish are produced by cold shock treatments. Likewise, the size of horizontal cell nucleus in inner nuclear layer of retina, ganglion cell nucleus in ganglion cell layer of retina, proximal tubule cell of kidney, hepatocytes and nuclear height of midgut epithelium all appear to be significantly larger than diploid (P<0.05). On the other hand, retina thickness is larger in diploid than induced triploid (P<0.05). Induced triploid shows low density of cell number. Results of this study suggest that same characteristics in the induced triploid exhibiting larger cells and nucleus sizes with fewer number of cells than the diploid can be useful criteria for the distinction between diploid and induced triploid, and also the ploidy level in marine medaka.
Abstract
The influence of triploidization on cell and nucleus size characteristics of the same tissues of erythrocyte, retina, kidney, hepatocyte and midgut epithelium in marine medaka, Oryzias dancena has been determined histologically. Induced triploid fish are produced by cold shock treatments. Likewise, the size of horizontal cell nucleus in inner nuclear layer of retina, ganglion cell nucleus in ganglion cell layer of retina, proximal tubule cell of kidney, hepatocytes and nuclear height of midgut epithelium all appear to be significantly larger than diploid (P<0.05). On the other hand, retina thickness is larger in diploid than induced triploid (P<0.05). Induced triploid shows low density of cell number. Results of this study suggest that same characteristics in the induced triploid exhibiting larger cells and nucleus sizes with fewer number of cells than the diploid can be useful criteria for the distinction between diploid and induced triploid, and also the ploidy level in marine medaka.
참고문헌 (24)
- Aliah RS, "Effects of induced triploidy on tissue structure of some organs in ayu", Bull Japan Soc Sci Fish, 56, pp.569~575 (1990)
- Benefey TJ, "The physiology and behavior of induced triploid fishes", Rev Fish Sci, 7, pp.39~67 (1999)
- Cal RM, "Growth and gonadal development in diploid and induced triploid turbot, Scophthalmus maximus", Aquaculture, 251, pp.99~108 (2006)
- 조영선, "광염성 송사리 Oryzias dancena 성체 및 자어의 염분도 변화에 대한 내성", 한국어류학회지, 22(1), pp.9~16 (2010), 한국어류학회
- Cho YS, "Functional ability of cytoskeletal β-actin regulator to drive constitutive and ubiquitous expression of a fluorescent reporter throughout the life cycle of transgenic marine medaka Oryzias dancena", Trans Res, 20, pp.1333~1355 (2011)
- Dunham RA, "Transgenic Animals in Agriculture", pp.209~229 (1999), CAB International
- Felip A, "Induction of triploidy and gynogenesis in teleost fish with emphasis on marine species", Genetica, 111, pp.175~195 (2001)
- Hulata G, "Genetic manipulation in aquaculture: a review of stock improvement by classical and modern technologies", Genetica, 111, pp.155~173 (2001)
- Inoue K, "Asian medaka fishes offer new models for studying mechanisms of seawater adaptation", Comp Biochem Physiol, Part B, 136, pp.635~645 (2003)
- Kang CK, "Differential expression of branchial Na+", K+ -ATPase of two medaka species, Oryzias latipes and Oryzias dancena, with different salinity tolerances acclimated to fresh water, brackish water and seawater, 2008, pp.151, 566~575
- Kim DS, "Status of fisheries biotechnology", BioWave, 3, pp.3~ (2001)
- Ko MG, "Production of transgenic triploid marine medaka (Oryzias dancena) carrying red fluorescence protein transgene driven by myosin light chain 2 promoter", MS, pp.2013
- Lincoln R, "Induced triploid rainbows show commercial potential", Fish Farmer, 7, pp.30~32 (1984)
- Park I-S, "Comparison of some tissues in diploid and induced triploid hybrid between mud loach, Misgurnus mizolepis and cyprinid loach, M. anguillicaudatus", Dev Reprod, 4, pp.19~28 (2000)
- Park I-S, "Haematological and physiological characteristics of diploid and triploid in cherry salmon, Oncorhynchus masou", J Aquacult, 8, pp.21~29 (1995)
- Park I-S, "Anesthetic effects of clove oil and lidocaine-HCl on marine medak, Oryzias dancena", Lab Animal, 40, pp.45~51 (2011)
- Peruzzi S, "Production of meiotic gynogenetic and induced triploid sea bass, Dicentrarchus labrax L. 1. Performances, maturation and carcass quality", Aquaculture, 230, pp.41~64 (2004)
- Piferrer F, "Polyploid fish and shellfish: Production, biology and applications to aquaculture for performance improvement and genetic containment", Aquaculture, 293, pp.125~6156 (2009)
- Sezaki K, "Haematological parameters and erythrocyte enzyme activities associated with increase in ploidy status of the spinous loach, Cobitis biwae Jordan and Synder", J Fish Biol, 32, pp.149~150 (1988)
- Swarup H, "Effect of triploidy on the body size, general irfanization and cellular structure in Gasterosteus aculeatus (L)", J Genet, 56, pp.143~155 (1959)
- Swarup H, "The oxygen consumption of diploid and triploid Gasterosteus aculeatus (L)", J Genet, 56, pp.156~160 (1959)
- Takashi H, "Sensory Organs", pp.42~73 (1982), Kodansha Ltd
- Thorgaard GH, "Ploidy manipulation and performance", Aquaculture, 57, pp.57~64 (1986)
- Ueno K, "Induction of triploid carp and their haematological characteristics", Jap J Genet, 59, pp.585~591 (1984)