본문 바로가기
KERD
논문KCI 미등재

Multispectral intravital microscopy for simultaneous bright-field and fluorescence imaging of the microvasculature

2

조회

0

다운로드

0

인용

0

참고문헌

주제분류자연과학일반

초록

Intravital video microscopy permits the observation of microcirculatory blood flow. This often requires fluorescent probes to visualize structures and dynamic processes that cannot be observed with conventional bright-field microscopy. Conventional light microscopes do not allow for simultaneous bright-field and fluorescent imaging. Moreover, in conventional microscopes, only one type of fluorescent label can be observed. This study introduces multispectral intravital video microscopy, which combines bright-field and fluorescence microscopy in a standard light microscope. The technique enables simultaneous real-time observation of fluorescently-labeled structures in relation to their direct physical surroundings. The advancement provides context for the orientation, movement, and function of labeled structures in the microcirculation.

Abstract

Intravital video microscopy permits the observation of microcirculatory blood flow. This often requires fluorescent probes to visualize structures and dynamic processes that cannot be observed with conventional bright-field microscopy. Conventional light microscopes do not allow for simultaneous bright-field and fluorescent imaging. Moreover, in conventional microscopes, only one type of fluorescent label can be observed. This study introduces multispectral intravital video microscopy, which combines bright-field and fluorescence microscopy in a standard light microscope. The technique enables simultaneous real-time observation of fluorescently-labeled structures in relation to their direct physical surroundings. The advancement provides context for the orientation, movement, and function of labeled structures in the microcirculation.

참고문헌 (45)

  1. BARAA K. AL-KHAZRAJI, "A Simple “Streak Length Method” for Quantifying and Characterizing Red Blood Cell Velocity Profiles and Blood Flow in Rat Skeletal Muscle Arterioles", Microcirculation, 19(4), pp.327~335 (2012), Wiley[DOI]
  2. R. Alon, "From rolling to arrest on blood vessels : leukocyte tap dancing on endothelial integrin ligands and chemokines at sub-second contacts", Semin. Immunol, 14, pp.93~ (2002)
  3. Evelyne Beerling, "Intravital characterization of tumor cell migration in pancreatic cancer", IntraVital, 5(3), pp.e1261773~ (2016), Informa UK Limited[DOI]
  4. E. Beerling, "Intravital microscopy : new insights into metastasis of tumors", J. Cell Sci, 124, pp.299~ (2011)
  5. R. Bordy, "Microvascular endothelial dysfunction in rheumatoid arthritis", Nat. Rev. Rheumatol, 14, pp.404~ (2018)
  6. A. Celi, "Thrombus formation : direct real-time observation and digital analysis of Thrombus assembly in a living mouse by confocal and Widefield Intravital microscopy", J. Thromb. Haemost, 1, pp.60~ (2003)
  7. Yeon Woong Choo, "Recent advances in intravital microscopy for investigation of dynamic cellular behavior in vivo", BMB Reports, 53(7), pp.357~366 (2020), 생화학분자생물학회[DOI]
  8. D. Coling, "Principles and application of fluorescence microscopy", Curr. Protoc. Mol. Biol. Chapter, 14, pp.10~ (2001)
  9. G. Cox, "Biological confocal microscopy", Mater. Today, 5, pp.34~ (2002)
  10. D. De Backer, "Pathophysiology of microcirculatory dysfunction and the pathogenesis of septic shock", Virulence, 5, pp.73~ (2014)
  11. M. L. Ellsworth, "Measurement of hemoglobin oxygen saturation in capillaries", Am. J. Phys., 252, pp.H1031~ (1987)
  12. B. Furie, "Thrombus formation in vivo", J. Clin. Invest, 115, pp.3355~ (2005)
  13. F. Helmchen, "Deep tissue two-photon microscopy", Nat. Methods, 2, pp.932~ (2005)
  14. J. James, "Biomedical Light Microscopy" (2012), Springer Science & Business Media
  15. G. H. Janssen, "Spontaneous leukocyte rolling in venules in untraumatized skin of conscious and anesthetized animals", Am. J. Physiol., 267, pp.H1199~ (1994)
  16. S.A. Japee, "Automated method for tracking individual red blood cells within capillaries to compute velocity and oxygen saturation", Microcirculation, 12, pp.507~ (2005)
  17. S.A. Japee, "A new video image analysis system to study red blood cell dynamics and oxygenation in capillary networks", Microcirculation, 12, pp.489~ (2005)
  18. C. N. Jenne, "The use of spinning-disk confocal microscopy for the Intravital analysis of platelet dynamics in response to systemic and local inflammation", PLoS One, 6, pp.e25109~ (2011)
  19. H. Jiang, "Altered macular microvasculature in mild cognitive impairment and Alzheimer disease", J. Neuro-Ophthalmol, 38, pp.292~ (2018)
  20. Arzu Karaman Koç, "Severe OSAS causes systemic microvascular dysfunction: Clinical evaluation of ninety‐eight OSAS patients", Clinical Otolaryngology, 44(3), pp.412~415 (2019), Wiley[DOI]
  21. A. G. Koutsiaris, "A velocity profile equation for blood flow in small arterioles and venules of small mammals in vivo and an evaluation based on literature data", Clin. Hemorheol. Microcirc, 43, pp.321~ (2009)
  22. K. Ley, "Chapter 11. Intravital microscopic investigation of leukocyte interactions with the blood vessel wall", Methods Enzymol., 445, pp.255~ (2008)
  23. W. Li, "Intravital 2-photon imaging of leukocyte trafficking in beating heart", J. Clin. Invest, 122, pp.2499~ (2012)
  24. H. H. Lipowsky, "Microvascular rheology and hemodynamics", Microcirculation, 12, pp.5~ (2005)
  25. O. Mahmoud, "Machine-learning-based functional microcirculation analysis", AAAI, 34, pp.13326~ (2020)
  26. A. Nakano, "Spinning-disk confocal microscopy. A cutting-edge tool for imaging of membrane traffic", Cell Struct. Funct., 27, pp.349~ (2002)
  27. M. D. Nelson, "Coronary microvascular dysfunction and heart failure with preserved ejection fraction as female-pattern cardiovascular disease: the chicken or the egg?", Eur. Heart J., 39, pp.850~ (2018)
  28. M. Nitzan, "Pulse oximetry: fundamentals and technology update", Med. Devices(Auckland, N. Z. ), 7, pp.231~ (2014)
  29. M. G. A. Oude Egbrink, "Especially Polymorphonuclear leukocytes, but also monomorphonuclear leukocytes, roll spontaneously in venules of intact rat skin: involvement of E-selectin", J. Invest. Dermatol, 118, pp.323~ (2002)
  30. Saparna Pai, "Visualizing leukocyte trafficking in the living brain with 2-photon intravital microscopy", Frontiers in Cellular Neuroscience, 6 (2013), Frontiers Media SA[DOI]
  31. N. Percie du Sert, "The ARRIVE Guidelines 2.0: updated guidelines for reporting animal research", PLoS Biol, 18, pp.e3000410~ (2020)
  32. V. Pinho, "Intravital microscopy to study leukocyte recruitment in vivo", Methods Mol. Biol. (Clifton, NJ), 689, pp.81~ (2011)
  33. N. S. Rost, "Diffuse microvascular dysfunction and loss of white matter integrity predict poor outcomes in patients with acute ischemic stroke", J. Cereb. Blood Flow Metab, 38, pp.75~ (2018)
  34. A. Sckell, "Dorsal skinfold chamber preparation in mice: studying angiogenesis by intravital microscopy", Methods Mol. Biol. (Clifton, NJ), 1430, pp.251~ (2016)
  35. T. Shimozawa, "Improving spinning disk confocal microscopy by preventing pinhole cross-talk for intravital imaging", PNAS, 110, pp.3399~ (2013)
  36. Yokogawa technical report english Edition 17, "K. Shin-Ichiro", Confocal laser microscope scanner and CCD camera, ~ (2002)
  37. S. Stehbens, "Imaging intracellular protein dynamics by spinning disk confocal microscopy", Methods Enzymol, 504, pp.293~ (2012)
  38. C. D. A. Stehouwer, "Microvascular dysfunction and hyperglycemia: a vicious cycle with widespread consequences", Diabetes, 67, pp.1729~ (2018)
  39. G. J. Tangelder, "Velocity profiles of blood platelets and red blood cells flowing in arterioles of the rabbit mesentery", Circ. Res, 59(5), pp.505~514 (1986)[DOI]
  40. G. M. Tozer, "Intravital imaging of tumour vascular networks using multi-photon fluorescence microscopy", Adv. Drug Deliv. Rev, 57, pp.135~ (2005)
  41. R. F. Tuma, "Handbook of Physiology: Microcirculation" (2008), Academic Press
  42. K. Tyml, "Preparation of rat extensor Digitorum longus muscle for Intravital investigation of the microcirculation", Int. J. Microcirc. Clin. Exp, 10, pp.335~ (1991)
  43. A. L. van de Ven, "Integrated Intravital microscopy and mathematical modeling to optimize nanotherapeutics delivery to tumors", AIP Adv, 2, pp.011208~ (2012)
  44. Sevim Zaim, "COVID-19 and Multiorgan Response", Current Problems in Cardiology, 45(8), pp.100618~ (2020), Elsevier BV[DOI]
  45. W. G. Zijlstra, "Absorption spectra of human fetal and adult oxyhemoglobin, de-oxyhemoglobin, carboxyhemoglobin, and methemoglobin", Clin. Chem, 37, pp.1633~ (1991)

관련 논문AI추천

이 간행물의 다른 논문 (3건)