Ruvbl1 is Essential for Ciliary Beating during Xenopus laevis Embryogenesis
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The Ruvb-like AAA ATPase1 (Ruvbl1; also known as Pontin) is an evolutionary conserved protein belonging to the adenosine triphosphates associated with diverse cellular activities (AAA+) superfamily of ATPases. Ruvbl1 is a component of various protein supercomplexes and is involved in a variety of cellular activities, including chromatin remodeling, DNA damage repair, and mitotic spindle assembly however, the developmental significance of this protein is unknown and needs detailed investigation. We investigated the developmental significance of Ruvbl1 in multiciliated cells of the Xenopus laevis epidermis since ruvbl1 is expressed in the multiciliated cells and pronephros during X. laevis embryogenesis. The knockdown of ruvbl1 significantly impaired cilia-driven fluid flow and basal body polarity in the X. laevis epidermis compared to control embryos, but did not affect cilia morphology. Our results suggest that Ruvbl1 plays a significant role in embryonic development by regulating ciliary beating; however, further investigation is needed to determine the mechanisms involved.
Abstract
The Ruvb-like AAA ATPase1 (Ruvbl1; also known as Pontin) is an evolutionary conserved protein belonging to the adenosine triphosphates associated with diverse cellular activities (AAA+) superfamily of ATPases. Ruvbl1 is a component of various protein supercomplexes and is involved in a variety of cellular activities, including chromatin remodeling, DNA damage repair, and mitotic spindle assembly however, the developmental significance of this protein is unknown and needs detailed investigation. We investigated the developmental significance of Ruvbl1 in multiciliated cells of the Xenopus laevis epidermis since ruvbl1 is expressed in the multiciliated cells and pronephros during X. laevis embryogenesis. The knockdown of ruvbl1 significantly impaired cilia-driven fluid flow and basal body polarity in the X. laevis epidermis compared to control embryos, but did not affect cilia morphology. Our results suggest that Ruvbl1 plays a significant role in embryonic development by regulating ciliary beating; however, further investigation is needed to determine the mechanisms involved.
참고문헌 (16)
- Bereshchenko O, "Pontin is essential for murine hematopoietic stem cell survival", Haematologica-the Hematology Journal, 97, pp.1291~1294 (2012)
- Claudia Dafinger, "Targeted deletion of the AAA-ATPase Ruvbl1 in mice disrupts ciliary integrity and causes renal disease and hydrocephalus", Experimental and Molecular Medicine, 50, pp.1~17 (2018), 생화학분자생물학회
- Fabczak H, "Role of the novel Hsp90 co-chaperones in dynein arms’ preassembly", Int J Mol Sci, 20, pp.6174~ (2019)
- Gartner W, "The ATP-dependent helicase RUVBL1", TIP49a associates with tubulin during mitosis, 2003, pp.56, 79~93
- Hartill VL, "DNAAF1 links heart laterality with the AAA+ ATPase RUVBL1 and ciliary intraflagellar transport", Hum Mol Genet, 27, pp.529~545 (2018)
- Ishikawa T, "Axoneme structure from motile cilia", Cold Spring Harb Perspect Biol, 9, pp.a028076~ (2017)
- Izumi N, "AAA+ proteins RUVBL1 and RUVBL2 coordinate PIKK activity and function in nonsense-mediated mRNA decay", Sci Signal, 3, pp.ra27~ (2010)
- Jha S, "RVB1", RVB2: Running rings around molecular biology, 2009, pp.34, 521~533
- Jónsson ZO, "Rvb1p and Rvb2p are essential components of a chromatin remodeling complex that regulates transcription of over 5% of yeast genes", J Biol Chem, 276, pp.16279~16288 (2001)
- Lee C, "Functional partitioning of a liquid-like organelle during assembly of axonemal dyneins", eLife, 9, pp.e58662~ (2020)
- Leigh MW, "Primary ciliary dyskinesia (PCD): A genetic disorder of motile cilia", Transl Sci Rare Dis, 4, pp.51~75 (2019)
- Li Y, "Axonemal dynein assembly requires the R2TP complex component Pontin", Development, 144, pp.4684~4693 (2017)
- Meunier A, "Multiciliated cells in animals", Cold Spring Harb Perspect Biol, 8, pp.a028233~ (2016)
- Qiu XB, "An eukaryotic RuvB-like protein (RUVBL1) essential for growth", J Biol Chem, 273, pp.27786~27793 (1998)
- Walentek P, "What we can learn from a tadpole about ciliopathies and airway diseases: Using systems biology in Xenopus to study cilia and mucociliary epithelia", Genesis, 55, pp.e23001~ (2017)
- Werner ME, "Understanding ciliated epithelia: The power of Xenopus", Genesis, 50, pp.176~185 (2012)