Growth Inhibitory Effect of Palatine Tonsil-derived Mesenchymal Stem Cells on Head and Neck Squamous Cell Carcinoma Cells
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Objectives. Mesenchymal stem cells (MSCs) play an important role in the development and growth of tumor cells. However,the effect of human MSCs on the growth of human tumors is not well understood. The purpose of this study is to confirm the growth effect of palatine tonsil-derived MSCs (TD-MSCs) on head and neck squamous cell carcinoma (HNSCC) cell lines and to elucidate the mechanism of their action. Methods. TD-MSCs were isolated from patient with chronic tonsillitis and tonsillar hypertrophy. Two human HNSCC cell lines (PNUH-12 and SNU-899) were studied and cocultured with isolated palatine tonsil-derived MSC. The growth inhibitory effect of MSCs on HNSCC cell lines was tested through methylthiazolyldiphenyl-tetrazolium (MTT) assay. The apoptosis induction effect of MSCs on cell lines was assessed with flow cytometry and reverse transcriptase (RT)-PCR. Results. Palatine tonsil-derived MSCs exhibited a growth inhibitory effect on both cell lines. Cell cycle analysis showed an accumulation of tumor cells predominantly in G0/G1 phase with an increase in concentration of TD-MSCs, which was confirmed by increased mRNA expression of cell cycle negative regulator p21. Apoptosis of tumor cells increased significantly as concentration of cocultured TD-MSCs increased. Additionally, mRNA expression of caspase 3 was upregulated with increased concentration of TD-MSCs. Conclusion. TD-MSCs have a potential growth inhibitory effect on HNSCC cell lines in vitro by inducing apoptotic cell death and G1 phase arrest of cell lines.
Abstract
Objectives. Mesenchymal stem cells (MSCs) play an important role in the development and growth of tumor cells. However,the effect of human MSCs on the growth of human tumors is not well understood. The purpose of this study is to confirm the growth effect of palatine tonsil-derived MSCs (TD-MSCs) on head and neck squamous cell carcinoma (HNSCC) cell lines and to elucidate the mechanism of their action. Methods. TD-MSCs were isolated from patient with chronic tonsillitis and tonsillar hypertrophy. Two human HNSCC cell lines (PNUH-12 and SNU-899) were studied and cocultured with isolated palatine tonsil-derived MSC. The growth inhibitory effect of MSCs on HNSCC cell lines was tested through methylthiazolyldiphenyl-tetrazolium (MTT) assay. The apoptosis induction effect of MSCs on cell lines was assessed with flow cytometry and reverse transcriptase (RT)-PCR. Results. Palatine tonsil-derived MSCs exhibited a growth inhibitory effect on both cell lines. Cell cycle analysis showed an accumulation of tumor cells predominantly in G0/G1 phase with an increase in concentration of TD-MSCs, which was confirmed by increased mRNA expression of cell cycle negative regulator p21. Apoptosis of tumor cells increased significantly as concentration of cocultured TD-MSCs increased. Additionally, mRNA expression of caspase 3 was upregulated with increased concentration of TD-MSCs. Conclusion. TD-MSCs have a potential growth inhibitory effect on HNSCC cell lines in vitro by inducing apoptotic cell death and G1 phase arrest of cell lines.
참고문헌 (35)
- Krause DS, "Multi-organ, multi-lineage engraftment by a single bone marrow-derived stem cell", Cell, 105(3), pp.369~377 (2001)
- Hamada H, "Mesenchymal stem cells (MSC) as therapeutic cytoreagents for gene therapy", Cancer Sci, 96(3), pp.149~156 (2005)
- Khakoo AY, "Human mesenchymal stem cells exert potent antitumorigenic effects in a model of Kaposi’s sarcoma", J Exp Med, 203(5), pp.1235~1247 (2006)
- Pittenger MF, "Multilineage potential of adult human mesenchymal stem cells", Science, 284(5411), pp.143~147 (1999)
- Sasser AK, "Human bone marrow stromal cells enhance breast cancer cell growth rates in a cell line-dependent manner when evaluated in 3D tumor environments", Cancer Lett, 254(2), pp.255~264 (2007)
- Elzaouk L, "Anti-tumor activity of mesenchymal stem cells producing IL-12 in a mouse melanoma model", Exp Dermatol, 15(11), pp.865~874 (2006)
- Ramasamy R, "Mesenchymal stem cells inhibit proliferation and apoptosis of tumor cells: impact on in vivo tumor growth", Leukemia, 21(2), pp.304~310 (2007)
- Zhu W, "Mesenchymal stem cells derived from bone marrow favor tumor cell growth in vivo", Exp Mol Pathol, 80(3), pp.267~274 (2006)
- Caplan AI, "Mesenchymal stem cells", J Orthop Res, 9(5), pp.641~650 (1991)
- Dennis JE, "Differentiation potential of conditionally immortalized mesenchymal progenitor cells from adult marrow of a H-2Kb-tsA58 transgenic mouse", J Cell Physiol, 167(3), pp.523~538 (1996)
- Friedenstein AJ, "Bone marrow osteogenic stem cells: in vitro cultivation and transplantation in diffusion chambers", Cell Tissue Kinet, 20(3), pp.263~272 (1987)
- Friedenstein AJ, "Heterotopic of bone marrow: analysis of precursor cells for osteogenic and hematopoietic tissues", Transplantation, 6(2), pp.230~247 (1968)
- Owen M., "Marrow stromal stem cells", J Cell Sci Suppl, 10, pp.63~76 (1988)
- Wakitani S, "Myogenic cells derived from rat bone marrow mesenchymal stem cells exposed to 5-azacytidine", Muscle Nerve, 18(12), pp.1417~1426 (1995)
- Young RG, "Use of mesenchymal stem cells in a collagen matrix for Achilles tendon repair", J Orthop Res, 16(4), pp.406~413 (1998)
- Rubinstein P, "Stored placental blood for unrelated bone marrow reconstitution", Blood, 81(7), pp.1679~1690 (1993)
- Noth U, "Multilineage mesenchymal differentiation potential of human trabecular bone-derived cells", J Orthop Res, 20(5), pp.1060~1069 (2002)
- Sekiya I, "Adipogenic differentiation of human adult stem cells from bone marrow stroma (MSCs)", J Bone Miner Res, 19(2), pp.256~264 (2004)
- Zuk PA, "Multilineage cells from human adipose tissue: implications for cell-based therapies", Tissue Eng, 7(2), pp.211~228 (2001)
- De Bari C, "Multipotent mesenchymal stem cells from adult human synovial membrane", Arthritis Rheum, 44(8), pp.1928~1942 (2001)
- Djouad F, "Transcriptional profiles discriminate bone marrow-derived and synovium-derived mesenchymal stem cells", Arthritis Res Ther, 6, pp.1304~1315 (2005)
- Shih DT, "Isolation and characterization of neurogenic mesenchymal stem cells in human scalp tissue", Stem Cells, 23(7), pp.1012~1020 (2005)
- Rzhaninova AA, "Isolation and phenotypical characterization of mesenchymal stem cells from human fetal thymus", Bull Exp Biol Med, 139(1), pp.134~140 (2005)
- Ivanovski S, "Stem cells in the periodontal ligament", Oral Dis, 12(4), pp.358~363 (2006)
- Lee OK, "Isolation of multipotent mesenchymal stem cells from umbilical cord blood", Blood, 103(5), pp.1669~1675 (2004)
- Sarugaser R, "Human umbilical cord perivascular (HUCPV) cells: a source of mesenchymal progenitors", Stem Cells, 23(2), pp.220~229 (2005)
- Yen BL, "Isolation of multipotent cells from human term placenta", Stem Cells, 23(1), pp.3~9 (2005)
- Ku JL, "Establishment and characterization of human laryngeal squamous cell carcinoma cell lines", Laryngoscope, 109(6), pp.976~982 (1999)
- Byung-Joo Lee, "Change of the Expression of Human Telomerase Reverse TranscriptasemRNA and Human Telomerase RNA after Cisplatin and 5-FluorouracilExposure in Head and Neck Squamous Cell Carcinoma Cell Lines", Journal of Korean Medical Science, 22(S), pp.73~78 (2007), 대한의학회
- Ehtesham M, "Stem cell therapies for malignant glioma", Neurosurg Focus, 19(3), pp.5~ (2005)
- Lee HJ, "Brain transplantation of immortalized human neural stem cells promotes functional recovery in mouse intracerebral hemorrhage stroke model", STEM CELLS, 25(5), pp.1204~1212
- Mapara KY, "Stem cells as vehicles for the treatment of brain cancer", Neurosurg Clin N Am, 18(1), pp.71~80 (2007)
- Nakamizo A, "Human bone marrow-derived mesenchymal stem cells in the treatment of gliomas", Cancer Res, 65(8), pp.3307~3318 (2005)
- Singh G, "Sources of neuronal material for implantation", Neuropathology, 21(2), pp.110~114 (2001)
- Lu YR, "The growth inhibitory effect of mesenchymal stem cells on tumor cells in vitro and in vivo", Cancer Biol Ther, 7(2), pp.245~251 (2008)