The Effect of Nano-Silver on Allergic Rhinitis Model in Mice
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Objectives. Silver has long been known as a strong antimicrobial and disinfectant. Several types of nano-silver coated products have been developed. However, the antimicrobial and disinfectant characteristics of nano-silver have not been well studied. The aim of this study was to investigate the effect of nano-silver on allergic inflammation in a mouse model. Methods. Female BALB/C mice were sensitized by intraperitoneal injection of ovalbumin (OVA) and aluminium hydroxide on days 0, 7, 14, and 21. Mice were challenged with intranasal instillation of OVA. Nano-silver was also administered nasally prior to intranasal instillation of OVA. Severity of allergic rhinitis was assessed according to nasal symptoms, serum OVA-specific IgE level, interleukin (IL)-4, IL-10, and interferon (INF)-γ levels in nasal lavage fluid. Hematoxylin-eosin stain and periodic acid-Schiff stain were performed for evaluation of histological change. Results. Nano-silver attenuated manifestation of nasal symptoms in sensitized mice and inhibited production of OVA-specific IgE, IL-4, and IL-10, however, it had no effect on INF-γ level. In addition, the degree of inflammatory cell infiltration and goblet cell hyperplasia was attenuated by nano-silver. Conclusion. These results suggest that nano-silver may effectively reduce allergic inflammation in a mouse model of allergic rhinitis. Through its properties as an anti-inflammatory agent, nano-silver may be a useful therapeutic strategy.
Abstract
Objectives. Silver has long been known as a strong antimicrobial and disinfectant. Several types of nano-silver coated products have been developed. However, the antimicrobial and disinfectant characteristics of nano-silver have not been well studied. The aim of this study was to investigate the effect of nano-silver on allergic inflammation in a mouse model. Methods. Female BALB/C mice were sensitized by intraperitoneal injection of ovalbumin (OVA) and aluminium hydroxide on days 0, 7, 14, and 21. Mice were challenged with intranasal instillation of OVA. Nano-silver was also administered nasally prior to intranasal instillation of OVA. Severity of allergic rhinitis was assessed according to nasal symptoms, serum OVA-specific IgE level, interleukin (IL)-4, IL-10, and interferon (INF)-γ levels in nasal lavage fluid. Hematoxylin-eosin stain and periodic acid-Schiff stain were performed for evaluation of histological change. Results. Nano-silver attenuated manifestation of nasal symptoms in sensitized mice and inhibited production of OVA-specific IgE, IL-4, and IL-10, however, it had no effect on INF-γ level. In addition, the degree of inflammatory cell infiltration and goblet cell hyperplasia was attenuated by nano-silver. Conclusion. These results suggest that nano-silver may effectively reduce allergic inflammation in a mouse model of allergic rhinitis. Through its properties as an anti-inflammatory agent, nano-silver may be a useful therapeutic strategy.
참고문헌 (18)
- Slawson RM, "Bacterial interactions with silver", Biol Met, 3(3-4), pp.151~154 (1990)
- Shvedova AA, "Unusual inflammatory and fibrogenic pulmonary responses to single-walled carbon nanotubes in mice", Am J Physiol Lung Cell Mol Physiol, 289(5), pp.L698~L708 (2005)
- Magrez A, "Cellular toxicity of carbon-based nanomaterials", Nano Lett, 6(6), pp.1121~1125 (2006)
- Shin SH, "The effects of nano-silver on the proliferation and cytokine expression by peripheral blood mononuclear cells", Int Immunopharmacol, 7(13), pp.1813~1818 (2007)
- Chen X, "Nanosilver: a nanoproduct in medical application", Toxicol Lett, 176(1), pp.1~12 (2008)
- 박정숙, "Induction of oral tolerance to Japanese cedar pollen", ARCHIVES OF PHARMACAL RESEARCH, 24(6), pp.557~563 (2001), PHARMACEUTICAL SOC KOREA
- Murasugi T, "Oral administration of a T cell epitope inhibits symptoms and reactions of allergic rhinitis in Japanese cedar pollen allergen- sensitized mice", Eur J Pharmacol, 510(1-2), pp.143~148 (2005)
- Newacheck PW, "Prevalence and impact of multiple childhood chronic illnesses", J Pediatr, 124(1), pp.40~48 (1994)
- Chen HW, "Titanium dioxide nanoparticles induce emphysema-like lung injury in mice", FASEB J, 20(13), pp.2393~2395 (2006)
- Xia T, "Comparison of the abilities of ambient and manufactured nanoparticles to induce cellular toxicity according to an oxidative stress paradigm", Nano Lett, 6(8), pp.1794~1807 (2006)
- Cyrys J, "Elemental composition and sources of fine and ultrafine ambient particles in Erfurt, Germany", Sci Total Environ, 305(1-3), pp.143~156 (2003)
- Nel A, "Atmosphere, air pollution-related illness: effects of particles", Science, 308(5723), pp.804~806 (2005)
- Brunner TJ, "In vitro cytotoxicity of oxide nanoparticles: comparison to asbestos, silica, and the effect of particle solubility", Environ Sci Technol, 40(14), pp.4374~4381 (2006)
- 노지연, "Ecotoxicity of Silver Nanoparticles on the Soil Nematode Caenorhabditis elegans Using Functional Ecotoxicogenomics", ENVIRONMENTAL SCIENCE & TECHNOLOGY, 43(10), pp.3933~3940 (2009), AMER CHEMICAL SOC
- Feng QL, "A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus", J Biomed Mater Res, 52(4), pp.662~668 (2000)
- 장선혜, "Attenuation of allergic airway inflammation and hyperresponsiveness in a murine model of asthma by silver nanoparticles", INTERNATIONAL JOURNAL OF NANOMEDICINE, 5, pp.505~515 (2010), DOVE MEDICAL PRESS LTD
- Bhol KC, "Topical nanocrystalline silver cream suppresses inflammatory cytokines and induces apoptosis of inflammatory cells in a murine model of allergic contact dermatitis", Br J Dermatol, 152(6), pp.1235~1242 (2005)
- 신지현, "Effect of oral tolerance in a mouse model of allergic rhinitis", OTOLARYNGOLOGY-HEAD AND NECK SURGERY, 142(3), pp.370~375, MOSBY