The Expression of AGO2 and DGCR8 in Idiopathic Sudden Sensorineural Hearing Loss
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Objectives. The microRNAs have been implicated in the development and function of the inner ear, especially in contribu- tion to hearing. However, the impact of idiopathic sudden sensorineural hearing loss (SSNHL) on expression of miR- NA biogenesis-related components has not been established. To investigate the regulations of microRNA (miRNA) biogenesis-related components, argonaute 2 (AGO2) and DiGeorge syndrome critical region gene 8 (DGCR8) mRNA expression in SSNHL and to evaluate the value of clinical parameters on their expression. Methods. Thirty-seven patients diagnosed with SSNHL and fifty-one healthy volunteers were included in this study. We measured mRNA expression levels of AGO2 and DGCR8 in whole blood cells but erythrocytes of patients with SSNHL and controls, using reverse transcription and real-time polymerase chain reaction analysis. Results. The mRNA expression level of AGO2 is upregulated in SSNHL. The expression level of AGO2 was significantly correlated with that of DGCR8 in both patients with SSNHL and controls. Expression level of AGO2 in SSNHL was correlated with white blood cell counts. Conclusion. This study demonstrated for the first time that the AGO2 mRNA expression level was upregulated in SSNHL, suggesting its important role in pathobiology of SSNHL development.
Abstract
Objectives. The microRNAs have been implicated in the development and function of the inner ear, especially in contribu- tion to hearing. However, the impact of idiopathic sudden sensorineural hearing loss (SSNHL) on expression of miR- NA biogenesis-related components has not been established. To investigate the regulations of microRNA (miRNA) biogenesis-related components, argonaute 2 (AGO2) and DiGeorge syndrome critical region gene 8 (DGCR8) mRNA expression in SSNHL and to evaluate the value of clinical parameters on their expression. Methods. Thirty-seven patients diagnosed with SSNHL and fifty-one healthy volunteers were included in this study. We measured mRNA expression levels of AGO2 and DGCR8 in whole blood cells but erythrocytes of patients with SSNHL and controls, using reverse transcription and real-time polymerase chain reaction analysis. Results. The mRNA expression level of AGO2 is upregulated in SSNHL. The expression level of AGO2 was significantly correlated with that of DGCR8 in both patients with SSNHL and controls. Expression level of AGO2 in SSNHL was correlated with white blood cell counts. Conclusion. This study demonstrated for the first time that the AGO2 mRNA expression level was upregulated in SSNHL, suggesting its important role in pathobiology of SSNHL development.
참고문헌 (26)
- Stachler RJ, "Clinical practice guideline: sudden hearing loss", Otolaryngol Head Neck Surg, 146(3 Suppl), pp.S1~S35 (2012)
- Byl FM Jr, "Sudden hearing loss: eight years’ experience and suggested prognostic table", Laryngoscope, 94(5 Pt 1), pp.647~661 (1984)
- Zadeh MH, "Diagnosis and treatment of sudden-onset sensorineural hearing loss: a study of 51 patients", Otolaryngol Head Neck Surg, 128(1), pp.92~98 (2003)
- Yossepowitch O, "Sudden hearing loss following acute hepatitis", Postgrad Med J, 75(883), pp.309~312 (1999)
- Suckfull M, "Perspectives on the pathophysiology and treatment of sudden idiopathic sensorineural hearing loss", Dtsch Arztebl Int, 106(41), pp.669~675 (2009)
- Nam SI, "A polymorphism at -1607 2G in the matrix metalloproteinase-1 (MMP-1) increased risk of sudden deafness in Korean population but not at -519A", G in MMP-1, 2011, pp.121(1), 171~175
- Nam SI, "L4 receptor polymorphism is associated with increased risk of sudden deafness in Korean population", Life Sci, 78(6), pp.664~667 (2006)
- Bartel DP, "MicroRNAs: genomics, biogenesis, mechanism, and function", Cell, 116(2), pp.281~297 (2004)
- Gregory RI, "The Microprocessor complex mediates the genesis of microRNAs", Nature, 432(7014), pp.235~240 (2004)
- Sand M, "Expression levels of the microRNA maturing microprocessor complex component DGCR8 and the RNA-induced silencing complex (RISC) components argonaute-1, argonaute-2, PACT, TARBP1, and TARBP2 in epithelial skin cancer", Mol Carcinog, 51(11), pp.916~922 (2012)
- Groves AK, "The genetics of hair cell development and regeneration", Annu Rev Neurosci, 36, pp.361~381 (2013)
- Zhang Q, "Identifying microRNAs involved in degeneration of the organ of corti during age-related hearing loss", PLoS One, 8(4), pp.e62786~ (2013)
- Inchley CS, "Reduced Dicer expression in the cord blood of infants admitted with severe respiratory syncytial virus disease", BMC Infect Dis, 11, pp.59~ (2011)
- Haussecker D, "Human tRNA-derived small RNAs in the global regulation of RNA silencing", RNA, 16(4), pp.673~695 (2010)
- Smith KM, "miR-29ab1 deficiency identifies a negative feedback loop controlling Th1 bias that is dysregulated in multiple sclerosis", J Immunol, 189(4), pp.1567~1576 (2012)
- Schmittgen TD, "Analyzing real-time PCR data by the comparative C(T) method", Nat Protoc, 3(6), pp.1101~1108 (2008)
- Fire A, "Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans", Nature, 391(6669), pp.806~811 (1998)
- Kim B, "An essential microRNA maturing microprocessor complex component DGCR8 is up-regulated in colorectal carcinomas", Clin Exp Med, 14(3), pp.331~336 (2014)
- Zhang YN, "Expression of microRNA machinery proteins in different types of chronic rhinosinusitis", Laryngoscope, 122(12), pp.2621~2627 (2012)
- Mockenhaupt S, "When cellular networks run out of control: global dysregulation of the RNAi machinery in human pathology and therapy", Prog Mol Biol Transl Sci, 102, pp.165~242 (2011)
- Jaffe BF, "Clinical studies in sudden deafness", Adv Otorhinolaryngol, 20, pp.221~228 (1973)
- Saeki N, "Assessment of prognosis in sudden deafness", Acta Otolaryngol Suppl, 510, pp.56~61 (1994)
- Enache R, "Prognostic factors in sudden hearing loss", J Med Life, 1(3), pp.343~347 (2008)
- Wei X, "Analysis of prognostic factors for sudden sensorineural hearing loss", Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi, 25(13), pp.599~601 (2011)
- Masuda M, "Correlations of inflammatory biomarkers with the onset and prognosis of idiopathic sudden sensorineural hearing loss", Otol Neurotol, 33(7), pp.1142~1150 (2012)
- Kassner SS, "Proinflammatory and proadhesive activation of lymphocytes and macrophages in sudden sensorineural hearing loss", Audiol Neurootol, 16(4), pp.254~262 (2011)