Glycine max와 G. tomentella 간 종간잡종의 미숙배 배양시 식물체 분화

Abstract

Wild perenial types of genus Glycine, as sources of genetic diversity, have been recently employed in the soybeanbreding programs. This study was caried out to introduce some useful characters of wild perennial Glycine species into G. max.Parent materials used for interspecific hybridization were two G. max cultivars, Ulsankong and Baemkong, and two G. tomentellastrains having different diploid (2n=2x=38, 2n=2x=40). Their factors afecting plant regeneration of imature hybrid embryo cul-ture were investigated. As a result of in vitro embryo culture, B5 medium supplemented with BA 0.1 mg/l + NA 0.25 mg/l, or IBA0.1 mg/l + 2ip 0.25 mg/l as growth regulators was the most effective on plant diferentiation from imature interspecific hybridembryos. Although improved with prolonged time after pollination, plant diferentiation rate from the embryo culture was higher intwo comon cultivars, Baemkong and Ulsankong, than two G. tomentela strains, 2n=38 and 40, while that of their interspecifichybrid embryos was dependent on their combination. The best time for in vitro culture of the hybrid embryo from Baemkong andG. tomentela 2n=38 was estimated 15 days after their cross on the basis of plant regeneration and pod survival.