Characterization of Receptor-like Protein Kinase Gene Expressed During Plant Regeneration in Hordeum vulgare L.
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Abstract
In order to provide information on molecular events during plant regeneration, cDNA library was constructed using tissuesof the 6 day-old shoot formation (SF) cultures. Diferential hybridization method was used to isolate genes expressed diferentiallyduring plant regeneration. We characterized one clone encoding receptor-like protein kinase (HvRLK). Using northern analysis, theexpresion of HvRLK was highly increased from 3, 6 day-old SF cultures and expressed in the shoot of plantlet. Tissue specificexpression of HvRLK during different stages of shoot induction and callus development was identified by in situ hybridization. Highlevels of HvRLK expression were observed in day 6 SF cultures, where the signal was localized primarily in the developing shoot pri-mordial. The transcript of HvRLK gene was slightly increased 3 hrs after plant growth hormone [ABA, cytokinin (BAP), and GA3]treatment, but not detected after 12 hrs in ABA. The expression of HvRLK was high in BAP application. In the case of GA3 treatment,HvRLKhrs after treatment. Expresion pattern of HvRLK gene was determined in the leaves treated by wounding. The transcript level ofHvRLK was highly increased from 1 hr after treatment, and slightly decreased from 3 to 24 hrs after wounding treatment.
참고문헌 (27)
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