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분자육종법을 이용한 벼멸구 저항성 고품질 자포니카 벼 신품종 ‘안미’ 육성

A Brown Planthopper Resistant and High Grain Quality Rice Variety ‘Anmi’ Developed by Molecular Breeding Method

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초록

‘Anmi’ is a new BPH (Brown planthopper) resistant japonica rice cultivar possessing the Bph18 gene derived fromwild rice, Oryza australiensis and high yield potential with good grain quality. ‘Anmi’ was derived from a cross ‘Junam’ and‘IR65482-7-216-1-2’ by a molecular marker assisted backcross breeding. The introgression line ‘IR65482-7-216-1-2’, the sourceof the Bph18 gene, was used as the donor parent for BPH resistance and ‘Junam’, a BPH-susceptible elite japonica cultivar withgood grain quality, was used as the recurrent parent. ‘Anmi’ was developed by three times backcross the F1 plants with ‘Junam’. The heading date of ‘Anmi’ is August 15 in central plain area, which is 5 days later than that of ‘Hwaseong’. It has 77 cmin culm length, 21 cm in panicle length. The number of spikelets per panicle is more than that of ‘Hwaseong’ and 1,000grain-weight of brown rice is 22.1 g which is less than 22.7 g of ‘Hwaseong’. Milled rice kernel of ‘Anmi’ is clear in appearance,low in amylase content and excellent in palatability of cooked rice. ‘Anmi’ shows resistance to BPH, leaf blast disease, bacterialblight, rice stripe disease but susceptible to other virus disease and insect pest. The milled rice yield performance of ‘Anmi’ isabout 5.76 MT/ha in local adaptability test for three years. This cultivar is adaptable to central plain area of Korea.

Abstract

‘Anmi’ is a new BPH (Brown planthopper) resistant japonica rice cultivar possessing the Bph18 gene derived fromwild rice, Oryza australiensis and high yield potential with good grain quality. ‘Anmi’ was derived from a cross ‘Junam’ and‘IR65482-7-216-1-2’ by a molecular marker assisted backcross breeding. The introgression line ‘IR65482-7-216-1-2’, the sourceof the Bph18 gene, was used as the donor parent for BPH resistance and ‘Junam’, a BPH-susceptible elite japonica cultivar withgood grain quality, was used as the recurrent parent. ‘Anmi’ was developed by three times backcross the F1 plants with ‘Junam’. The heading date of ‘Anmi’ is August 15 in central plain area, which is 5 days later than that of ‘Hwaseong’. It has 77 cmin culm length, 21 cm in panicle length. The number of spikelets per panicle is more than that of ‘Hwaseong’ and 1,000grain-weight of brown rice is 22.1 g which is less than 22.7 g of ‘Hwaseong’. Milled rice kernel of ‘Anmi’ is clear in appearance,low in amylase content and excellent in palatability of cooked rice. ‘Anmi’ shows resistance to BPH, leaf blast disease, bacterialblight, rice stripe disease but susceptible to other virus disease and insect pest. The milled rice yield performance of ‘Anmi’ isabout 5.76 MT/ha in local adaptability test for three years. This cultivar is adaptable to central plain area of Korea.

참고문헌 (10)

  1. Jena KK, "High-resolution mapping of a new brown planthopper (BPH) resistance gene, Bph18(t), and marker-assisted selection for BPH resistance in rice (Oryza sativa L.)", Theor. Appl. Genet, 112, pp.288~297 (2006)
  2. Kaneda C., "Studies on breeding japonica rice resistant to the brown planthopper", Nilaparvata lugens Stal. Bul. National Agr. Res. Cen, 2, pp.1~74 (1984)
  3. Murray M, "Rapid isolation of high molecular weight plant DNA", Nucl. Acid Res, 8, pp.4321~ (1980)
  4. 신문식, "벼멸구 저항성 Bph18 유전자가 벼의 수량구성요소에 미치는 영향", 한국육종학회지, 43(1), pp.56~61 (2011), 한국육종학회
  5. Suh JP, "Development of elite breeding lines conferring Bph18gene-derived resistance to brown planthopper (BPH) by marker-assisted selection and genome-wide background analysis in japonica rice (Oryza sativa L.)", Field Crops Research, 120, pp.215~222 (2011)
  6. 2008 Project plan for collaborative research program to develop new variety summer crop, "RDA", 25~66 (2008)
  7. 2008 Project report for collaborative research program to develop new variety summer crop, "RDA", 9~181 (2008)
  8. 2009 Project report for collaborative research program to develop new variety summer crop, "RDA", 13~208 (2009)
  9. 2010 Project report for collaborative research program to develop new variety summer crop, "RDA", 5~175 (2010)
  10. Xiong LZ, "Identification of genetic factors controlling domesticationrelated traits of rice using an F2 population of a cross between Oryza sativa and O. rufipogon", Theor. Appl. Genet, 98, pp.243~251 (1999)

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