미나리 종자번식 체계 확립을 위한 최적 채종단계, 층적처리 및 종자 세척방법 구명
Optimum Seed Maturity, Stratification and Washing Method for Establishment of Seed Propagation System in Water Dropwort (Oenanthe stolonifera DC)
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초록
미나리의 종자번식체계를 확립을 위해 종자의 최적 채종단계 구명과 층적처리 방법 및 세척방법을 구명하고자 연구를수행하였다. 미나리 종자는 성숙한 종자일수록 발아율이 향상되었으며, 특히 개화 후 40일에 채종 된 종자의 발아율이 가장 높았다. 또한 층적처리를 8주동안 실시하였을 때 가장 효과적이었다. 모래를 이용하여 층적처리 된 종자가 모래와 혼합하지 않고 수확한 종자를 바로 저온저장 한 종자보다 발아율이 높았으며, 층적처리 한 종자를 변온 조건인 노지에 매장하여 저장하였을 때 4℃저장고에 항온 저장하였을 때보다 발아율이 19 % 향상되었다. 그리고 층적처리 된 종자를 노지에매장하여 8주 동안 변온저장 하였을 때 발아율이 가장 높았다. 종피의 발아억제물질은 0.5% sodium hypochlo-rite와 0.0005% vital oxide를 각각 3분과 60분 동안 침지한 후 세척하였을 때 가장 효과적이었다. sodium hypochlo-rite의 경우 0.5∼1.0% 용액으로 약3∼5분 동안 세척하였을 때 발아율이 향상되었고, 2.0% 용액으로 세척하였을 때는 발아율이저하되는 경향을 나타내었다. 또한 vital oxide 0.0005%의 농도로 60분 동안 세척하였을 때 발아율이 향상되었다.
Abstract
The influence of seed maturity, stratification, and seed washing method on seed germination were investigated for establishment of seed propagation system in dropwort (Oenanthe stolonifera DC). Seed germination rate was increased as period of the seed ripening increased, especially the seed over 40 days after flowering showed the highest germination rate. The optimum period of cold stratification treatment to promote seed germination was about 8 weeks. The seed treated by a cold wet stratification with sand exhibited higher germination rate compared to the seed treated by a cold wet stratification without sand. The stratified seeds that were stored in the ground having fluctuating temperature were improved up to 19%, while stratification stored under constant temperature(4℃) did not improve germination rate. In addition, 8 weeks of stratification period under fluctuating temperature in the ground was most effective. Sodium hypochlorite, vital oxide, and distilled water were used as chemicals to remove the germination inhibitor of seed coat. Water washing of seed coat after soaking in 0.5% sodium hypochlorite and 0.0005% vital oxide for three minutes and 60 minutes respectively could effectively remove the germination inhibitors in the seed coat.
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