Agrobacterium을 이용한 제초제 저항성 옥수수 형질전환체 생산
Production of Transgenic Maize Plants with Herbicide Resistance Through Agrobacterium-mediated Transformation
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초록
Maize is the most important grain crop in the world. The genetic engineering technology has been used to enhance the various agronomical traits. The maize transformation is a crucial step for application of gene technologies to improve maize. The choice of genotype and explant material influences on the transformation efficiency and the production of stable transgenic plants. Immature embryos of Hi IIA were infected with Agrobacterium tumefaciens LBA4404 including superbinary vector (bar and GUS or GFP genes). The transformation efficiency was based on the transgenic calli induction from immature embryos on selection medium with 3 mg/L bialaphos. The transformation efficiency varied from 1.01 to 2.74%. The integration and expression of bar, GUS, and GFP genes were confirmed in T0 and T1 generations of transgenic plants using genomic PCR and bar strip test. Also herbicide resistance in T1 transgenic plants was shown when leaves and whole plants were treated with Basta. These results suggest that the successful Agrobacterium-mediated transformation of Hi IIA will improve further opportunities for maize functional genomic and genome editing studies.
Abstract
Maize is the most important grain crop in the world. The genetic engineering technology has been used to enhance the various agronomical traits. The maize transformation is a crucial step for application of gene technologies to improve maize. The choice of genotype and explant material influences on the transformation efficiency and the production of stable transgenic plants. Immature embryos of Hi IIA were infected with Agrobacterium tumefaciens LBA4404 including superbinary vector (bar and GUS or GFP genes). The transformation efficiency was based on the transgenic calli induction from immature embryos on selection medium with 3 mg/L bialaphos. The transformation efficiency varied from 1.01 to 2.74%. The integration and expression of bar, GUS, and GFP genes were confirmed in T0 and T1 generations of transgenic plants using genomic PCR and bar strip test. Also herbicide resistance in T1 transgenic plants was shown when leaves and whole plants were treated with Basta. These results suggest that the successful Agrobacterium-mediated transformation of Hi IIA will improve further opportunities for maize functional genomic and genome editing studies.
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