Phylogenetic Relationships among Wild, Weedy and Cultivated Rices based on F₁Fertility, Isozyme and RAPD Variation

Abstract

In order to know the phylogenetic relationships betwen wild, weedy and cultivated rice, half diallel crosses among nineaccessions including a wild rice Oryza rufipogon W130, thre long grain weedy rices Cheongdoaengmi 1 from Korea, C9575 fromJapan, BT5d from Bhutan, and thre short grain weedy rices Kyeongsanaengmi 14 from Korea, C81 and C79-1 from China, anindica cultivar IR36, and a japonica cultivar Nagdongbyeo were made and the F1s were evaluated in the field for sed fertility.Allozyme variations at 11 loci from six isozymes and RAPD variations in 64 loci from 37 primers of the wild, weedy and cultivatedrices were evaluated. High sed fertility was found in F1s among the long grain weedy strains, and the F1s between long grainweedy strains with the wild rice strain W130 and the indica cultivar IR36 also showed high percent of seed set . However, the F1fertility among the short grain weedy rices showed variation in acordance with the origin. The F1 fertility of the short grain weedyrice accessions with the wild rice or the japonica cultivar Nagdongbyeo was also diferent in accordance with the origin. The testedmaterials were classified into two groups based on isozyme and RAPD variation. The long grain weedy rices, the wild rice W130and the indica cultivar IR36 were clasified into one group and the short grain weedy rices and the japonica cultivar Nagdongbyeointo the other. RAPD variation was biger than isozyme variation, and RAPD markers seemed to be more effective for classifyingrice varieties. F1 fertility, isozyme and RAPD did not produce the consistent variation pattern, thus, employing more than onemarker system is more efective than a single marker for classifying rice varieties. Kye words : Weedy rice, Phylogenetic relationship, F1 fertility, Isozyme, RAPD. .