밀양23호/O. rufipogon 교잡 유래 근동질유전자 계통 이용 QTLs 분석

Abstract

A near-isogenic line CR1815, containing three introgressed segments on chromosomes 1, 7 and 9 from O. rufipogoninto the Oryza sativa cultivar Milyang23 background was developed based on MAS (marker aided selection) and backcrossing.-acterize and fine map the gemes/QTLs, 142 BC4F3 lines from CR 1815 were evaluated for 12 traits in two locations. QTL analysiswas carried out to determine the relationship between marker genotype and the phenotypes. Twenty significant QTLs were identi-fied for yield and yield components using the single point analysis. Twelve and fourteen QTLs were detected in Daejeon andSuwon, respectively. Six QTLs were detected in common in two locations, and these include two QTLs each for culm length, pan-icle length and yield per plant. The phenotypic variation explained by each QTL ranged from 4.9 to 35.6%. At four out of the sixQTL loci detected in two locations in common, the O. rufipogon alleles were beneficial in the Milyang 23 background. Allelesfrom the O. rufipogon parent were associated with positive effect on yield at QTL loci yd1 and yd9.