Inter Simple Sequence Repeat (ISSR) Marker를 이용한 구기자의 유전적 유연관계 분석
Assessment of Genetic Relationship among Boxthorn (Lycium chinensis Mill.) Accessions by ISSR Markers
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Abstract
This study was conducted to assess genetic diversity among 16 genotypes of boxthorn (Lycium chinensis Mill.) using inter-simple sequence repeat (ISSR) markers. The 18 ISSR primers out of 100 primers showed the amplification of 101 reproducible fragments. A total of 56 DNA fragments were polymorphic with an average 3.1 polymorphic bands per primer. The polymorphic primers were divided into 16 anchored primers and 2 non-anchored primers. All of the anchored primers were di-nucleotide repeat motif, and polymorphism level was higher in the primers with poly GA and CT motif than CA and GT motif primers. Based on polymorphism, cluster analysis was conducted by the unweighted pair-group method with arithmetic average (UPGMA) methods. Sixteen boxthorn varieties and accessions were separated into 2 distinctive groups and genetic distance of cluster ranged from 0.82 to 0.97. Eighteen markers were able to distinguish every variety. Therefore, ISSR markers may be suitable for characterizing the large numbers of germplasms and fingerprinting of boxthorn varieties.
참고문헌 (19)
- Blair MW, "Inter-simple sequence repeat (ISSR) amplification for analysis of microsatellite motif frequency and fingerprinting in rice (Oryza sativa L.).", 98, pp.780~792 (1999)
- Fang DQ, "Identification of closely related citrus cultivars with inter-simple sequence repeat markers", 95, pp.408~417 (1997)
- Hu J, "analysis of sweetpotato and wild relative using Inter-simple sequence repeats (ISSRs).", 53, pp.297~304 (2003)
- Kim DH, "Genetic relationships of sesame germplasm collection as revealed by inter-simple sequence repeats.", 121, pp.259~262 (2002)
- Lee BC, "Variation of chemical properties in collected boxthorn varieties", 30, pp.267~272 (1998)
- Lee EJ, "Varietal difference of black fruit rot in boxthorn", 33, pp.45~49 (2001)
- Nagaoka T, "Applicability of inter- simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers", 94, pp.597~602 (1997)
- Park JS, "Genetic similarity of boxthorn varieties", 32, pp.117~121 (2000)
- Park SY, "Identification and classification Lycium chinense Mill.", 28, pp.221~226 (1996)
- Prevost A, "A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivar", 98, pp.107~112 (1999)
- Qian W, "Genetic variation with and among populations of a wild rice Oryza granulata from China detected by RAPD and ISSR markers", 102, pp.440~449 (2001)
- Rakoczy-Trojanowska M, "Characteristics and a comparison of three classes of microsatellite- based markers and their application in plants", 9, pp.221~238 (2004)
- Rohlf FJ, "NTSYSpc. Numerical Taxonomy and Multivariate Analysis System- Version 2.10b" (2000), Applied Biostatistics Inc. New York.
- Scott KD, "Microsatellites derived from ESTs, and their comparison with those derived by other methods", pp.225~237 (2001), CABI Publ., Oxon.
- Seo GS, "Lee JI 1987. Varietal differences of the physiological and ecological character of boxthorn. Korean J. Breed. 19", pp.81~90
- Smulders MJM, "Use of short microsatellites from database sequences to generate polymorphims among Lycopersicon esculentum cultivars and accessions of other Lycopersicon species", 97, pp.264~272 (1997)
- Sneath PHA, "The Principles and Practice of Numerical Classification"
- Souframanien J, "A comparative analysis of genetic diversity in blackgram genotypes using RAPD and ISSR markers", 109, pp.1687~1693 (2004)
- Staub JE, "Genetic markers, map construction, and their application in plant breeding", 31, pp.729~741 (1996)