Effect of pinewood nematode on the water content and early disease development of seedlings of susceptible Pinus densiflora and resistant Pinus × rigitaeda for breeding for resistance to pinewood nematode
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Three-year-old seedlings of susceptible Pinus densiflora and resistant Pinus x rigitaeda were each inoculated with the pinewood nematode, Bursaphelenchus xylophilus, to compare disease development. Needle dehydration was evident on seedlings of P. densiflora by 20 days after inoculation, 10 days earlier than this symptom was observed on P. × rigitaeda. Xylem drying was more frequent in seedlings of P. densiflora than in that of P. × rigitaeda between 20 and 60 days after inoculation. No significant differences were found between P. densiflora and P. × rigitaeda for stem water content or for stem and leaf relative water content in current-year branches after nematode inoculation, but the average number of B. xylophilus recovered from stems differed significantly between the two groups. The number of B. xylophilus recovered from stems was negatively correlated with the stem water content and with stem and leaf relative water content. By the time the experiment was terminated at 60 days after inoculation, all 3 of the last group of P. densiflora seedlings had died, but 2 of the 3 remaining P. × rigitaeda hybrid seedlings were still alive. Additional studies are needed to further explore the specific mechanisms preventing nematode multiplication in the seedlings of resistant P. × rigitaeda.
Abstract
Three-year-old seedlings of susceptible Pinus densiflora and resistant Pinus x rigitaeda were each inoculated with the pinewood nematode, Bursaphelenchus xylophilus, to compare disease development. Needle dehydration was evident on seedlings of P. densiflora by 20 days after inoculation, 10 days earlier than this symptom was observed on P. × rigitaeda. Xylem drying was more frequent in seedlings of P. densiflora than in that of P. × rigitaeda between 20 and 60 days after inoculation. No significant differences were found between P. densiflora and P. × rigitaeda for stem water content or for stem and leaf relative water content in current-year branches after nematode inoculation, but the average number of B. xylophilus recovered from stems differed significantly between the two groups. The number of B. xylophilus recovered from stems was negatively correlated with the stem water content and with stem and leaf relative water content. By the time the experiment was terminated at 60 days after inoculation, all 3 of the last group of P. densiflora seedlings had died, but 2 of the 3 remaining P. × rigitaeda hybrid seedlings were still alive. Additional studies are needed to further explore the specific mechanisms preventing nematode multiplication in the seedlings of resistant P. × rigitaeda.
참고문헌 (29)
- Dropkin VH, "Pine wilt in the U.S. In Proceedings of the United States - Japan Seminar: The resistance mechanisms of pines against pine wilt disease", pp.3~5 (1984), Columbia, Extension Publ.
- Futai K, "The variety of resistances among pine-species to pinewood nematode, Bursaphelenchus lignicolus", Bull. Kyoto Univ. For., 51, pp.23~36 (1979)
- Hara N, "Histological analysis for mechanism of pine wilt disease", J. Jpn. For. Soc., 88, pp.364~369 (2006)
- Ikeda T, "Water relations, xylem embolism and histological features of Pinus thunbergii inoculated with virulent or avirulent pine wood nematode, Bursaphelenchus xylophilus", J. Exp. Bot., 46(285), pp.441~449 (1995)
- Ishii K, "Resistance of interspecific hybrids among the pine species belonging to Sylvestres subsect. to pine wood nematode (Bursaphelenchus lignicolus)", Tokyo: Jpn. For. Soc., pp.291~292 (1981)
- Jones JT, "Bursaphelenchus xylophilus: opportunities in comparative genomics and molecular host-parasite interactions", Mol. Plant Pathol., 9(3), pp.357~368 (2008)
- Kuroda K, "Mechanism of cavitation development in the pine wilt disease", Eur. J. For. Path., 21, pp.82~89 (1991)
- families selected as resistant to pine wilt, "J. For. Res.", 2004, 217~224
- Kuroda K, "Bursaphelenchus xylophilus-induced pine wilt: factors associated with resistance", Eur. J. For. Path., 21, pp.430~438 (1991)
- Kuroda K, "Effects of cavitation on the development of pine wilt disease caused by Bursaphelenchus xylophilus", Annals of the Phytopathological Society of Japan, 54, pp.606~615 (1988)
- 권태성, "Distribution patterns of Monochamus alternatus and M. saltuarius(Coleoptera: Cerambycidae) in Korea", 한국임학회지, 95(5), pp.543~550 (2006), 한국임학회
- 이영진, "ominant Height Growth and Site Index Curves for Pitch-Ioblolly Hybrid Pine (Pinus rigida x P. taeda) Plantation", 한국임학회지, 91(5), pp.661~666 (2002), 한국임학회
- Mamiya Y, "Pine wood nematode, Bursaphelenchus lignicolus Mamiya and Kiyohara, as a causal agent of pine wilting disease", Rev. Plant Prot. Res., 5, pp.46~60 (1972)
- Mamiya Y, "Reproduction of pine lethal wilting disease by the inoculation of young trees with Bursaphelenchus lignicolus", Jpn. J. Nematol., 2, pp.40~44 (1972)
- Mamiya Y, "Inoculation of the first year pine (Pinus densiflora) seedlings with the pine wood nematode Bursaphelenchus lignicolus and histopathology of diseased seedlings", J. Jpn. For. Soc., 62, pp.176~183 (1980)
- Mamiya Y, "Pathology of the pine wilt disease caused by Bursaphelenchus xylophilus", Annu. Rev. Phytopathol., 21, pp.201~220 (1983)
- Mamiya Y, "Transmission of Bursaphelenchus lignicolus (Nematoda: Aphelenchoididae) by Monochamus alternatus (Coleoptera: Cerambycidae)", Nematologica, 18, pp.159~162 (1972)
- Mori Y, "Relationship between resistance to pine wilt disease and the migration or proliferation of pine wood nematodes", Eur. J. Plant Pathol., 122, pp.529~538 (2008)
- Sasaki M, "The initial performances of interspecific hybrids, Pinus thunbergii × P. massoniana and P. tabulaeformis", Trans. Meet. Jpn. For. Soc., pp.183~184 (1976)
- Southey JF, "Laboratory methods for work with plant and soil nematodes" (1986), HMSO, London
- SYSTAT Software Inc, "Statistics II" (2004), SYSTAT Software Inc., Richmond, CA
- Tamura H, "Blockage of water conduction in Pinus thunbergii inoculated with Bursaphelenchus xylophilus", Jpn. J. Nematol., 17, pp.23~30 (1987)
- Tan JJ, "Research advance in pathogenic mechanism of pine wood nematode disease", Jour. Huazhong Agric. Univ., 22, pp.613~617 (2003)
- Tan JJ, "A study on disease development and early diagnosis of pine wood nematode, Bursaphelenchus xylophilus, infection of Japanese black pine", Nematol., 7, pp.481~485 (2005)
- Trudgill DL, "Resistance to and tolerance of plant parasitic nematodes in plants", Annu. Rev. Phytopathol., 29, pp.167~192 (1991)
- Wang YY, "A study on difference of pH between healthy and pine wilt diseased woods of several pine species", Acta Phytopathologica Sinica, 31, pp.342~348 (2001)
- Wingfield MJ, "Pathogenicity of Bursaphelenchus xylophilus in pines in Minnesota and Wisconsin", J. Nematol., 18, pp.44~49 (1986)
- Kwan-Soo Woo, "Variation in Susceptibility of Pine Species Seedlings with the Pine WoodNematode, Bursaphelenchus xylophilus, in Greenhouse", The Plant Pathology Journal, 23(2), pp.70~75 (2007), 한국식물병리학회
- Yamada T, "Biochemical responses in pines infected with Bursaphelenchus xylophilus", J. Jpn. For. Soc., 88, pp.370~382 (2006)