고추 매운맛 함량 연구를 위한 F7 RIL 집단의 원예적 특성 평가
Evaluation of Horticultural Characteristics in F7 RIL Populations for Pungency Level Studies in Chili Pepper
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
The unique trait ‘pungency’ in peppers (Capsicum.) results from the secondary metabolites known as capsaicinoids. Previous studies have identified a single dominant gene, Pun1, to be involved in biosynthesis of capsaicin. Molecular markers to distinguish between presence and absence of pungency have already been developed. But there have been little studies and information concerning the genetic control of quantitative trait of capsaicinoid content in Capsicum fruit. The purpose of this study aimed cultivation and evaluation of F7 RIL population for QTL analysis related to the capsaicinoid content. We have evaluated several horticultural characteristics including the capsaicinoid content in two parents and 93 plants of F7 generation. The capsaicinoid content of pungent parent, Saengryeg 211 was found to be 341.6 mg/100 g, which was higher than pungent cultivar, ‘Chungyang’. For the non-pungent parent, Saengryeg 213, it was 0mg/100 g. This coincidence with molecular marker related to presence/absence of pungency. The capsaicinoid content in 93 plants of F7 generation ranges from 77.8 mg/100 g to 1046.0 mg/100 g and its distribution in pungent lines showed a normal curve pattern. These results provide a strong foundation of this mapping population for further QTL analysis studies related to fruit quality including the pungency level.
Abstract
The unique trait ‘pungency’ in peppers (Capsicum.) results from the secondary metabolites known as capsaicinoids. Previous studies have identified a single dominant gene, Pun1, to be involved in biosynthesis of capsaicin. Molecular markers to distinguish between presence and absence of pungency have already been developed. But there have been little studies and information concerning the genetic control of quantitative trait of capsaicinoid content in Capsicum fruit. The purpose of this study aimed cultivation and evaluation of F7 RIL population for QTL analysis related to the capsaicinoid content. We have evaluated several horticultural characteristics including the capsaicinoid content in two parents and 93 plants of F7 generation. The capsaicinoid content of pungent parent, Saengryeg 211 was found to be 341.6 mg/100 g, which was higher than pungent cultivar, ‘Chungyang’. For the non-pungent parent, Saengryeg 213, it was 0mg/100 g. This coincidence with molecular marker related to presence/absence of pungency. The capsaicinoid content in 93 plants of F7 generation ranges from 77.8 mg/100 g to 1046.0 mg/100 g and its distribution in pungent lines showed a normal curve pattern. These results provide a strong foundation of this mapping population for further QTL analysis studies related to fruit quality including the pungency level.
참고문헌 (27)
- Andrews J., "Peppers, The Domesticated Capsicums" (1995), University of Texas Press
- Bennet DJ, "Constitution and biosynthesis of capsaicin", J. Chem. Soc, pp.442~446 (1968)
- Ben Chaim A, "QTL analysis for capsaicinoid content in Capsicum", Theor. Appl. Genet, 113, pp.1481~1490 (2006)
- Blum E, "Molecular mapping of the C locus for presence of pungency in Capsicum", Genome, 45, pp.702~705 (2002)
- Blum E, "Molecular mapping of capsaicinoid biosynthesis genes and QTL analysis for capsaicinoid content in Capsicum", Theor. Appl. Genet, 108, pp.79~86 (2003)
- 조병철, "홍고추 생육시기별 기상여건과 내적 품질과의 상관관계", 시설원예‧식물공장, 13(2), pp.67~72 (2004), 한국생물환경조절학회
- Charles S, "The Pun1 gene for pungency in pepper encodes a putative acyltransferase", Plant J, 42, pp.675~688 (2005)
- 정구민, "시기를 달리하여 일시 수확한 고추의 등급별 품질", 한국식품과학회지, 34(5), pp.919~923 (2002), 한국식품과학회
- Curl AL, "The carotenoids of red bell peppers", J. Agr. Food Chem, 10, pp.504~509 (1962)
- Curl AL, "The carotenoids of green bell peppers", J. Agr. Food. Chem, 12, pp.522~526 (1964)
- Deshpande RB, "Studies in Indian chillies: (4) Inheritance of pungency in Capsicum annuum L", Ind. Jour. Agr. Sci, 5, pp.513~516 (1934)
- Fujiwake H, "Intracellular localization of capsaicin and capsaicinoid in the protoplast of Capsicum fruit", Plant Cell Physiol, 21, pp.1023~1030 (1980)
- Ha SH, "A comparison of the carotenoid accumulation in Capsicum varieties that show different ripening colours : deletion of the capsanthin-capsorubin synthase gene is not a prerequisite for the formation of a yellow pepper", J. Exp. Bot, 58, pp.3135~3144 (2007)
- Hoffman PG, "Separation and quantitation of red pepper major heat principles by reverse high pressure liquid chromatogrphy", J. Agric Food Chem, 31, pp.1326~1330 (1983)
- Hong SH, "The situations of quality control system for red-pepper powder in Korea and America", J. Korean Capsicum Res. Coop, 4, pp.95~101 (1995)
- Hong SH, "The situations of quality control system for red pepper powder in Korea and American", J. Korean Capsicum Res. Coop, 4, pp.93~99 (1996)
- Huh JH, "A candidate gene approach identified phytoene synthase as the locus for mature fruit color in red pepper (Capsicum spp.)", Theor. Appl. Genet, 102, pp.524~530 (2001)
- Kang BC, "Combined molecular linkage map construction using RFLP and AFLP in hot pepper", Theor. Appl. Genet, 102, pp.531~539 (2001)
- "The method of surveying characteristics in pepper (Capsicum spp.)" (2008)[URL]
- Lang L-Q, "Orange fruit color in Capsicum due to deletion of capsanthincapsorubin synthesis gene", Korean J. Breed. Sci, 54, pp.33~39 (2004)
- Byung-Dong Kim, "Non-pungent Capsicum Contains a Deletion in the Capsaicinoid Synthetase Gene, which Allows Early Detection of Pungency with SCAR Markers", Molecules and Cells, 19(2), pp.262~267 (2005), 한국분자세포생물학회
- Lefebvre V, "Construction of an intraspecific integrated linkage map of pepper using molecular markers and doubled-haploid progenies", Genome, 38, pp.112~121 (1995)
- Loaiza-Figueroa F, "Genetics of a second locus determining pungency in chilli peppers (Capsicum)", J. Hered, 79, pp.314~315 (1988)
- Livingstone KD, "Genome mapping in Capsicum and the evolution of genome structure in the Solanaceae", Genetics, 152, pp.1183~1202 (1999)
- Sheh MT, "Comparative analyses of genetic diversities within tomato and pepper collections detected by retrotransposon-based SSAP, AFLP and SSR", Theor. Appl. Genet, 110, pp.819~831 (2005)
- Shin HH, "Quality atrributes of Korean red pepper according to cultivars and growing areas", Korean J. Food Sci. Technol, 23, pp.296~300 (1991)
- Todd PH, "Determination of pungency due to capsicum by gas-liquid chromatography", J. Food Sci, 42, pp.660~665 (1977)