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포도 무핵 유전자원의 유연관계 분석 및 무핵 형질과 연관된 P3_VvAGL11 마커의 활용성 평가

Analysis of Genetic Relationship of Seedless Germplasm and Validation Assay of the P3_VvAGL11 Marker Linked to Seedlessness in Grapevines

정성민|정찬진|남종철|노정호|마경호|박교선|허윤영
한국육종학회 (The Korean Society of Breeding Science)|2014.03|한국육종학회지 (KOREAN JOURNAL OF BREEDING SCIENCE)|제46권 제1호|pp. 28-36|DOI http://dx.doi.org/10.9787/KJBS.2014.46.1.028

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참고문헌

키워드

ValidationGenetic diversityUPGMAGrapevineSeedlessness
주제분류농학

초록

The genetic relationships of twenty seedless grape cultivars, used as parents for seedless grape breeding, were analyzedand the validation assay of the P3_VvAGL11 marker related to the seedlessness was examined. Total 218 polymorphic bandswere generated by 30 SSR markers. The average number of alleles per marker was 7.3. The polymorphism information contents(PIC) averaged 0.442, value ranging from 0.052 to 0.083 by each SSR marker. Eighteen seedless grape cultivars derived from‘Sultanina’ and ‘Kishmish Chernyi’ had 196-bp alleles of the P3_VvAGL11 associated with seedlessness. The unweightedpair-group method arithmetic average (UPGMA) cluster analysis was performed using selected 218 polymorphic bands. And fourgroups were derived on the similarity index 0.722. Each group accorded with the classification by the ratio of the Europeanspecies (Vitis vinifera), the American species (V. labrusca) and the inter-specific hybrid between the two species (V. labruscana). Similarity values among the tested seedless grape cultivars were ranged from 0.592 for ‘Beauty Seedless’ and ‘Honey Seedless’to 0.844 for ‘Emerald Seedless’ and ‘Ruby Seedless’, the average similarity value was 0.715. These results could be used asuseful reference for selecting parents with diverse backgrounds and early selection of seedless seedlings in breeding program ofseedless grape cultivars.

Abstract

The genetic relationships of twenty seedless grape cultivars, used as parents for seedless grape breeding, were analyzedand the validation assay of the P3_VvAGL11 marker related to the seedlessness was examined. Total 218 polymorphic bandswere generated by 30 SSR markers. The average number of alleles per marker was 7.3. The polymorphism information contents(PIC) averaged 0.442, value ranging from 0.052 to 0.083 by each SSR marker. Eighteen seedless grape cultivars derived from‘Sultanina’ and ‘Kishmish Chernyi’ had 196-bp alleles of the P3_VvAGL11 associated with seedlessness. The unweightedpair-group method arithmetic average (UPGMA) cluster analysis was performed using selected 218 polymorphic bands. And fourgroups were derived on the similarity index 0.722. Each group accorded with the classification by the ratio of the Europeanspecies (Vitis vinifera), the American species (V. labrusca) and the inter-specific hybrid between the two species (V. labruscana). Similarity values among the tested seedless grape cultivars were ranged from 0.592 for ‘Beauty Seedless’ and ‘Honey Seedless’to 0.844 for ‘Emerald Seedless’ and ‘Ruby Seedless’, the average similarity value was 0.715. These results could be used asuseful reference for selecting parents with diverse backgrounds and early selection of seedless seedlings in breeding program ofseedless grape cultivars.

참고문헌 (36)

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