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약배양 이용 벼멸구, 흰잎마름병 및 줄무늬잎마름병 저항성 복합 내병충성 벼 계통 육성

opment of Multi-resistant Lines to Brown Planthopper, Bacterial Blight, and Rice Stripe Virus using Anther Culture in Rice

김기영|김보경|이종희|박종호|노태환|정지웅|김우재|남정권|백만기|조영찬|백소현|유재수|백채훈|이점호|하기용|박현수
한국육종학회 (The Korean Society of Breeding Science)|2014.03|한국육종학회지 (KOREAN JOURNAL OF BREEDING SCIENCE)|제46권 제1호|pp. 78-89|DOI http://dx.doi.org/10.9787/KJBS.2014.46.1.078

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참고문헌

키워드

RiceAnther cultureMulti-resistantSegregation distortionLinkage drag
주제분류농학

초록

This study was conducted to develop multi-resistant lines to brown planthopper, bacterial blight, and rice stripe virususing anther culture in rice. A total of 213 double haploid lines were developed the cross between HR26234-12-1-1 conferringresistant to bacterial blight and rice stripe virus and SR30071-3-7-23-6-2-1-1 conferring resistant to brown planthopper, bacterialbight, and rice stripe virus. Using DNA molecular marker, HR26234 and SR30071 were confirmed to have Xa3+xa5+Stvb-i andBph18+Xa4+Stvb-i, respectively. All double haploid lines carried Stvb-i, and Bph18+Xa3, Bph18+Xa4, Bph18+Xa3+xa5,Bph18+Xa4+xa5, bph18+Xa3, bph18+Xa4, bph18+Xa3+xa5, and bph18+Xa4+xa5 combinations were identified. Segregationdistortions such as no combinations carrying Bph18(or bph18)+xa5+Stvb-i and fewer lines carrying Bph18 than bph18 wereoccurred in DH population. Brown planthopper resistant lines carrying Bph18 showed longer culm length than susceptible lines. Selected Bph18+Xa4+xa5+Stvb-i combination lines with short culm conferred resistant to brown planthopper, bacterial blight,and rice stripe virus, while showed deleterious effects such as spikelet sterility, lower yield, and vulnerable to lodging thanstandard and comparative varieties. Using anther culture, we rapidly developed multi-resistant lines to brown planthopper,bacterial blight, and rice stripe virus. However, distorted segregation in DH population and linkage drag with Bph18 wereobstacles to develop practical multi-resistant cultivars.

Abstract

This study was conducted to develop multi-resistant lines to brown planthopper, bacterial blight, and rice stripe virususing anther culture in rice. A total of 213 double haploid lines were developed the cross between HR26234-12-1-1 conferringresistant to bacterial blight and rice stripe virus and SR30071-3-7-23-6-2-1-1 conferring resistant to brown planthopper, bacterialbight, and rice stripe virus. Using DNA molecular marker, HR26234 and SR30071 were confirmed to have Xa3+xa5+Stvb-i andBph18+Xa4+Stvb-i, respectively. All double haploid lines carried Stvb-i, and Bph18+Xa3, Bph18+Xa4, Bph18+Xa3+xa5,Bph18+Xa4+xa5, bph18+Xa3, bph18+Xa4, bph18+Xa3+xa5, and bph18+Xa4+xa5 combinations were identified. Segregationdistortions such as no combinations carrying Bph18(or bph18)+xa5+Stvb-i and fewer lines carrying Bph18 than bph18 wereoccurred in DH population. Brown planthopper resistant lines carrying Bph18 showed longer culm length than susceptible lines. Selected Bph18+Xa4+xa5+Stvb-i combination lines with short culm conferred resistant to brown planthopper, bacterial blight,and rice stripe virus, while showed deleterious effects such as spikelet sterility, lower yield, and vulnerable to lodging thanstandard and comparative varieties. Using anther culture, we rapidly developed multi-resistant lines to brown planthopper,bacterial blight, and rice stripe virus. However, distorted segregation in DH population and linkage drag with Bph18 wereobstacles to develop practical multi-resistant cultivars.

참고문헌 (31)

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