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장수형 밀 계통의 특성 분석 II. 밀가루 및 관련 유전자 특성

Characterization of Korean Wheat Line with Long Spike. II. Flour Characteristics and Genetic Variations

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초록

국내 밀 수량성 향상을 위해 이삭 길이가 길고 1수립수가많은 장수형 계통의 품질과 이들 특성과 관련된 표지인자에대한 평가를 수행하였다. 장수형 164계통의 회분, 단백질, 침전가, 습부율, 밀가루 입자 크기와 밀가루 색택 밝기의 평균은금강밀보다 회분과 단백질함량이 높으며 습부율은 높고, 밀가루 밝기와 입자 크기는 비슷하였으나 침전가는 낮게 나타났다. 단백질의 질적 특성에서 단백질함량은 침전가, 습부율, 밀가루 입자 크기와 고도의 정의 상관을 보였고, 밀가루의 밝기와는 부의 상관을 나타냈다. 품질특성 관련 표지인자를 평가한 결과, 장수형 164계통은 모두 Glu-A3c, Wx-A1a, Wx-B1a, Wx-D1a의 유전자형을 나타내 금강밀과 동일하였다. 장수형계통은 주로 Glu-A1c, Glu-B1c, Glu-D1a, Glu-B3g, Pina- D1b와 Pinb-D1b의 유전자형이 많았다. Glu-A1b과 Glu-B1b 유전자형은 단백질함량이 많고, 습부율이 높은 특성을 나타냈다. Glu-D1d, Glu-B3h과 Pinb-D1b 유전자형은 다른 유전자형에 비해 회분과 단백질함량이 많으며, 침전가와 습부율이높고, 밀가루 입자가 크고, 밀가루의 색이 어두운 특성을 보였다. 이러한 결과를 통해서 장수형 계통은 수량성 증대를 위해필요하고, 향후 용도별 품질 우수 자원의 특성을 집적하여 용도별 다수성 밀 품종개발을 위한 자원으로 활용하고자 한다.

Abstract

Molecular markers related to flour qualities of long spike wheat lines were evaluated. Ash, wet gluten and protein content of long spike wheat lines were higher than Keumkang(Korean wheat cv.) but SDS-sedimentation volume(SDSF) was lower. Particle size and lightness of flour of long spike wheat lines showed similar to Keumkang. The high positively correlation was presented between protein content and SDSF, wet gluten and particle size, respectively. However, negatively correlation was between protein content and lightness. Through the analysis of molecular markers, we identified the genotypes of four genes, Glu-A3c, Wx-A1a, Wx-B1a and Wx-D1a. These genes had same genotypes with Keumkang. Six genes, Glu-A1c, Glu-B1c, Glu-D1a, Glu-B3g, Pina-D1and Pinb-D1b, were presented in most of the lines. Two genes, Glu-A1 and Glu-B1b, were related to higher protein contents and wet gluten. The lines containing three genotypes, Glu-D1d, Glu-B3h and Pinb-D1b, had higher ash, protein contents, SDSF and wet gluten, and lightness was darker. Through the results, we expect that long spike type wheat lines are necessary to breed wheat having high yield productivity.

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