‘신동진’ 유전배경에 벼흰잎마름병 저항성 유전자 Xa21이 도입된 복합내병성 중만생 벼 ‘참동진’
The Multiple Disease-resistant, Mid-late Maturing Rice Cultivar ‘Chamdongjin’, Carrying the Bacterial Blight Resistance Gene Xa21, with the Genetic Background of ‘Sindongjin’
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The rice cultivar ‘Chamdongjin’ was developed to enhance the disease resistance of ‘Sindongjin’. ‘Sindongjin’, developed by theRDA in 1999, is a mega-variety with a unique grain size and excellent taste, and has been cultivated in the largest area in Korea since2018. As ‘Sindongjin’ has been cultivated in a large area recently, problems such as pest damage are increasing, and the necessity to developalternative cultivars is emerging. Accordingly, the multiple disease-resistant, mid-late maturing rice cultivar ‘Chamdongjin’, carrying the bacterialblight resistance gene Xa21 into the genetic background of ‘Sindongjin’, was developed. ‘Chamdongjin’ was developed by the backcrossingmethod using the donor parent ‘HR27195-59-3-5-5’, harboring the bacterial blight resistance genes Xa3+Xa21 and the recurrent parent‘Sindongjin’. ‘Chamdongjin’ was selected through the pedigree method, yield trials, and local adaptability tests, with a high selection pressurefor agronomic trait similarities with ‘Sindongjin’. In order to effectively improve the disease resistance, marker-assisted selection for diseaseresistance genes and bioassays for bacterial blight, blast, and stripe virus were performed in parallel. ‘Chamdongjin’ was confirmed to havethree disease-resistance genes, Xa3+Xa21+Stvb-i. Most of agronomic traits of ‘Chamdongjin’ were similar to ‘Sindongjin’, while ‘Chamdongjin’showed enhanced disease resistance against bacterial blight and panicle blast compared to ‘Sindongjin’. ‘Chamdongjin’, like ‘Sindongjin’,possessed a grain size-related gene, the gs3 allele, and exhibited larger grains than general Korean japonica cultivars. In addition, ‘Chamdongjin’showed a similar or higher level of palatability compared to ‘Sindognjin’, indicating the excellent quality characteristics. In the genetic backgroundanalysis using 787 KASP markers, ‘Chamdongjin’ showed 96.3% homozygosity with ‘Sindongjin’, indicating that it had a more similar geneticbackground to ‘Sindongjin’ than to other existing cultivars. ‘Chamgdongjin’ is expected to be widely cultivated as an alternative to ‘Sindongjin’,which could maintain the brand value while preventing the disease damage caused to ‘Sindongjin’ (Registration No. 9310).
Abstract
The rice cultivar ‘Chamdongjin’ was developed to enhance the disease resistance of ‘Sindongjin’. ‘Sindongjin’, developed by theRDA in 1999, is a mega-variety with a unique grain size and excellent taste, and has been cultivated in the largest area in Korea since2018. As ‘Sindongjin’ has been cultivated in a large area recently, problems such as pest damage are increasing, and the necessity to developalternative cultivars is emerging. Accordingly, the multiple disease-resistant, mid-late maturing rice cultivar ‘Chamdongjin’, carrying the bacterialblight resistance gene Xa21 into the genetic background of ‘Sindongjin’, was developed. ‘Chamdongjin’ was developed by the backcrossingmethod using the donor parent ‘HR27195-59-3-5-5’, harboring the bacterial blight resistance genes Xa3+Xa21 and the recurrent parent‘Sindongjin’. ‘Chamdongjin’ was selected through the pedigree method, yield trials, and local adaptability tests, with a high selection pressurefor agronomic trait similarities with ‘Sindongjin’. In order to effectively improve the disease resistance, marker-assisted selection for diseaseresistance genes and bioassays for bacterial blight, blast, and stripe virus were performed in parallel. ‘Chamdongjin’ was confirmed to havethree disease-resistance genes, Xa3+Xa21+Stvb-i. Most of agronomic traits of ‘Chamdongjin’ were similar to ‘Sindongjin’, while ‘Chamdongjin’showed enhanced disease resistance against bacterial blight and panicle blast compared to ‘Sindongjin’. ‘Chamdongjin’, like ‘Sindongjin’,possessed a grain size-related gene, the gs3 allele, and exhibited larger grains than general Korean japonica cultivars. In addition, ‘Chamdongjin’showed a similar or higher level of palatability compared to ‘Sindognjin’, indicating the excellent quality characteristics. In the genetic backgroundanalysis using 787 KASP markers, ‘Chamdongjin’ showed 96.3% homozygosity with ‘Sindongjin’, indicating that it had a more similar geneticbackground to ‘Sindongjin’ than to other existing cultivars. ‘Chamgdongjin’ is expected to be widely cultivated as an alternative to ‘Sindongjin’,which could maintain the brand value while preventing the disease damage caused to ‘Sindongjin’ (Registration No. 9310).
참고문헌 (39)
- Official methods for analysis, "Association of Official Analytical Chemists(AOAC)", Association of official analytical chemists, ~ (2000)
- 백만기, "도열병 내구 저항성 자포니카 벼품종 팔공의 저항성 관련 유전좌위 분석", 한국육종학회지, 51(4), pp.395~403 (2019), 한국육종학회[DOI]
- Kyeong-Seong Cheon, "Kompetitive Allele-Specific PCR Marker Development and Quantitative Trait Locus Mapping for Bakanae Disease Resistance in Korean Japonica Rice Varieties", Plant Breeding and Biotechnology, 7(3), pp.208~219 (2019), 한국육종학회[DOI]
- Cheo ZR, "Optimum conditions for alkali digestivity test in rice", Korean J Crop Sci, 19, pp.7~13 (1975)
- 최성호, "한국의 벼 흰잎마름병 내구저항성 유전자 동정", 한국육종학회지, 35(4), pp.283~288 (2003), 한국육종학회
- Jena KK, "Development of 25 near-isogenic lines(NILs)with ten BPH resistance genes in rice(Oryza sativa L. ) : production, resistance spectrum, and molecular analysis", Theor Appl Gent, 130, pp.2345~2360 (2017)
- Juliano BO, "rice chemistry and technology", pp.59~120 (1985), The American association of cereal chemists
- 강위수, "우리나라 남부지방에서의 2014년 벼 이삭도열병 대발생", 식물병 연구, 25(4), pp.196~204 (2019), 한국식물병리학회[DOI]
- Kauffman HE, "An improved technique for evaluating resistance of rice varieties to Xanthomonas oryzae", Plant Dis Rep, 57, pp.537~541 (1973)
- Khush GS, "A new gene for resistance to bacterial Blight from O. longistaminata", Rice Genet Newsl, 7, pp.121~122 (1990)
- 김기영, "Development of Near-Isogenic Lines (NILs) Conferring Xa4, xa5 and Xa21 Genes Resistant to Bacterial Blight (Xanthomonas oryzae pv. oryzae) in japonica rice Genetic Background", 한국육종학회지, 43(5), pp.383~390 (2011), 한국육종학회
- Kim MS, "Marker-assisted backcrossing(MABc)to improve eating quality with thin seed coat and aleurone layer of non-glutinous japonica variety in rice", Genes, 13, pp.210~225 (2022)
- Kim SS, "Physical characteristics of chalky kernels and their effects on sensory quality of cooked rice", Cereal Chem, 73, pp.376~379 (2000)
- 김성섭, "농촌진흥기관 개발 품종의 경제적 파급효과 분석", 한국산학기술학회논문지, 22(6), pp.155~166 (2021), 한국산학기술학회[DOI]
- Kumar S, "MEGA X : Molecular evolutionary genetics analysis across computing platforms", Mol Biol Evol, 35, pp.1547~1549 (2018)
- 곽도연, "망실을 이용한 벼 바이러스병 저항성 대량 검정체계", 한국작물학회지, 52(2), pp.129~133 (2007), 한국작물학회
- 이춘기, "도정효율 증진을 위한 벼 품종특성별 현미선별체 적정크기", 한국작물학회지, 54(4), pp.357~365 (2009), 한국작물학회
- 이창민, "국내 육성 벼 품종 입형 관련 특성 분석", 한국작물학회지, 65(3), pp.199~213 (2020), 한국작물학회
- Lee CW, "Development and application of target capture sequencing SNP-genotyping platform in rice", Genes, 13, pp.794~806 (2022)
- Lee SW, "Distribution of Xanthomoans oryzae pv. oryzae strains virulent to Xa21 in Korea", Phytopathol, 89, pp.928~933 (1999)
- "Pest monitoring. Rice" (2022)[URL]
- 박현수, "벼흰잎마름병 균계 K1 및 K3a 접종에 따른 조생종 벼 품종의 수량성 및 품질변이", 한국육종학회지, 44(4), pp.516~525 (2012), 한국육종학회[DOI]
- 박현수, "벼흰잎마름병균에 대한 인디카와 자포니카 벼의 단일 저항성 유전자 반응과 이들의 집적 효과", 한국육종학회지, 45(2), pp.119~129 (2013), 한국육종학회[DOI]
- 박현수, "평야지 적응성 향상을 위한 벼흰잎마름병 및 줄무늬잎마름병 저항성 유전자 집적 조생 계통 개발", 한국육종학회지, 47(2), pp.118~127 (2015), 한국육종학회[DOI]
- 박현수, "표현형 및 분자표지 선발 이용 벼흰잎마름병 저항성 유전자 집적자포니카 벼 계통 개발", 한국육종학회지, 48(2), pp.140~158 (2016), 한국육종학회[DOI]
- 박현수, "자포니카 장립종 장원형 벼 계통 개발 및 특성분석", 한국육종학회지, 50(2), pp.116~130 (2018), 한국육종학회[DOI]
- 박현수, "벼흰잎마름병 저항성 유전자 집적 고품질 중만생 벼 ‘신진백’", 한국육종학회지, 51(3), pp.263~276 (2019), 한국육종학회[DOI]
- 박현수, "운광벼 유전배경의 복합내병성 조생 벼 ‘아이에스592비비’", 한국육종학회지, 52(4), pp.473~484 (2020), 한국육종학회[DOI]
- Park NK, "Classification of rice cultivars by the rice leaf blast reaction and its utilization for breeding", Ph. D, pp.1993
- Standard of analysis and survey for agricultural research, "Rural Development Administration(RDA)", 315~338 (2012)
- Project plant for collaborative research program to develop new variety summer crop, "Rural Development Administration(RDA)", 1~46 (2018)
- Project plant for collaborative research program to develop new variety summer crop, "Rural Development Administration(RDA)", 3~50 (2019)
- Project plant for collaborative research program to develop new variety summer crop, "Rural Development Administration(RDA)", 3~50 (2020)
- 노재환, "고품질 벼 품종의 도열병 저항성", 한국육종학회지, 39(3), pp.288~295 (2007), 한국육종학회
- 신문식, "벼흰잎마름병 저항성 품종 육성 및 금후 연구 방향", 한국육종학회지, 43(4), pp.241~251 (2011), 한국육종학회
- Shin YJ, "Identification of garin size-related QTLs in Korean japonica rice using genome resequencing and high-throughput image analysis", Agriculture, 12, pp.51~66 (2022)
- Song WY, "A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21", Science, 270, pp.1804~1806 (1995)
- Voorrips RE, "MapCart : software for the graphical presentation of linkage maps and QTLs", J Hered, 93, pp.77~78 (2002)
- Wang GL, "The cloned gene, Xa21, confers resistance to multiple Xanthomonas oryzae pv. oryzae isolates in transgenic plants", Mol Plant-Microbe Interact, 12, pp.103~108 (1996)