마이토마이신 C가 흰 쥐의 속눈썹 재성장에 미치는 영향
Effects of Mitomycin C on Eyelash-Regrowth in Epilated Rats
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목적: 본 연구는 마이토마이신 C가 흰 쥐의 속눈썹 재성장에 어떠한 영향을 미치는가를 알아보기 위해 시행하였다. 대상과 방법: 열 마리의 흰 쥐의 우측 눈꺼풀과 좌측 눈꺼풀에 각각 0.04% 마이토마이신 C와 생리식염수를 한 방울씩 점적 후 양측의속눈썹을 제거하였고, 속눈썹을 제거한 후 3개월 뒤 재성장한 속눈썹의 숫자를 확인하고 양측의 눈꺼풀 조직을 광학현미경 및 전자현미경 하에서 관찰하였다. 결과: 재성장한 속눈썹은 우측이 1.0 ± 0.81개, 좌측이 12.5 ± 1.08개였으며, 재성장률은 우측이 7.35 ± 5.55%, 좌측이 98.45 ±3.27%였다. 조직학적 검사에서 광학현미경하에서는 우측의 모낭에서 모간의 소실이 발견되는 점 외에는 좌측과 큰 차이를 보이지 않았으나, 전자현미경 관찰 시 우측 눈꺼풀의 모낭에서 사립체의 파괴를 관찰할 수 있었으며 좌측에서는 관찰되지 않았다. 결론: 흰 쥐에서 0.04% 마이토마이신 C의 점적 후 속눈썹의 단순 제거가 흰 쥐 속눈썹의 재성장을 억제하는 것으로 보이며 이러한결과는 마이토마이신 C가 눈꺼풀의 변형이 없는 속눈썹증 환자에서 침습적 치료를 대신할 수 있는 방법을 연구함에 있어 도움을 줄것으로 생각한다.
Abstract
Purpose: The aim of the present study was to determine the effects of mitomycin C (MMC) on eyelash regrowth in rats. Methods: Ten rats were subjected to bilateral eyelid epilation. One drop of 0.04% MMC solution and one drop of normal saline was then administered to the right and left eyelid, respectively. Three months post-epliation, the number of regrown eyelashes was counted, and a section of tissue from each eyelid was examined under light- and electron microscopies. Results: The number of regrown long eyelashes was 1.0 ± 0.81 on the right, and 12.5 ± 1.08 on the left. The rate of regrowth was 7.35 ± 5.55% on the right and 98.45 ± 3.27% on the left. Histological examination revealed no significant differences between the right and left eyelids, except for the absence of any hair shafts in the follicles on the right. However,electron microscopy revealed mitochondrial damage in the follicles of the right eyelid. These changes were not observed in tissue from the left eyelid. Conclusions: The application of 0.04% MMC inhibited the regrowth of normal eyelashes in rats following simple epilation. These results suggest that MMC may be a novel therapy for replacing invasive surgery in trichiasis with no malpositioning.
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