배양된 사람 코점막 섬유아세포에 대한 양막추출물의 효과
Effect of Amniotic Membrane Extract on Cultured Human Nasal Mucosa Fibroblasts
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
목적: 양막추출물이 배양된 사람의 코점막 섬유아세포의 생존에 미치는 효과에 대해 알아보고자 하였다. 대상과 방법: 일차배양한 사람의 비점막 섬유아세포를 0, 10, 20, 30 μg/mL의 양막추출물에 3일간 노출시킨 후 섬유아세포의 생존은 MTT assay로, 세포의 자가소멸은 annexin-V/propidium iodide 이중염색 후 유세포분석으로 평가하였다. 결과: 양막추출물은 10 μg/mL의 농도에서부터 섬유아세포의 증식을 유의하게 억제시켰으며(p=0.000), 유세포분석에서 10 μg/mL의 농도에서부터 섬유아세포의 자가소멸을 유의하게 증가시켰다(p=0.024). 결론: 양막추출물은 생체 외 환경에서 섬유아세포의 증식을 억제하였으며, 세포 자가소멸이 어느 정도 그 기전에 연관성이 있는 것으로 보인다. 그러므로 코경유 눈물주머니코안연결술 시 양막추출물의 보조적인 사용은 수술 결과를 향상시킬 수 있을 것으로 보인다.
Abstract
Purpose: To investigate the effects of amniotic membrane extract (AME) on the survival of cultured human nasal mucosa fibroblasts. Methods: Primary cultured human nasal mucosa fibroblasts were exposed to 0, 10, 20, or 30 μg/mL AME for 3 days. The survival of the human nasal mucosa fibroblasts was measured using the MTT assay and apoptosis was evaluated with flow cytometry using annexin-V/propidium iodide double staining. Results: AME decreased significantly in fibroblast proliferation after exposure to 10 μg/mL (p = 0.000), and caused significant apoptosis of the fibroblasts after exposure to 10 μg/mL (p = 0.024). Conclusions: AME decreased fibroblast proliferation in vitro at least through induction of apoptosis. Therefore, adjuvant use of AME during endonasal dacryocystorhinostomy may improve clinical outcomes.
참고문헌 (32)
- Allen K., "Dacryocystorhinostomy failure : association with nasolacrimal silicone intubation", Ophthalmic Surg, 20, pp.486~489 (1989)
- Zolli CL., "Dacryocystorhinostomy : a review of 119 cases", Ophthalmic Surg, 13, pp.905~910 (1982)
- McLachlan DL., "Results of dacryocystorhinostomy : analysis of the reoperations", Ophthalmic Surg, 11, pp.427~430 (1980)
- Tarbet KJ., "External dacryocystorhinostomy, Surgical success, patient satisfaction, and economic cost", Ophthalmology, 102, pp.1065~1070 (1995)
- Walland MJ., "Factors affecting the success rate of open lacrimal surgery", Br J Ophthalmol, 78, pp.888~891 (1994)
- Kao SC., "Dacryocystorhinostomy with intraoperative mitomycin C", Ophthalmology, 104, pp.86~91 (1997)
- Yalaz M., "Use of mitomycin C and 5-fluorouracil in external dacryocystorhinostomy", Orbit, 18, pp.239~245 (1999)
- 김정홍, "부비동 내시경 수술 후 GUARDIX-SL？의 유착방지 효과", 대한이비인후과학회지 두경부외과학, 48(12), pp.1478~1483 (2005), 대한이비인후과학회
- 최영재, "비강내 누낭비강문합술시 시행한 양막충전술의 단기 임상 결과", 대한안과학회지, 49(3), pp.384~389 (2008), 대한안과학회
- De Rötth A., "Plastic repair of conjunctival defects with fetal membranes", Arch Ophthalmol, 23, pp.522~525 (1940)
- Tseng SC., "Amniotic membrane transplantation for conjunctival surface reconstruction", Am J Ophthalmol, 124, pp.765~774 (1997)
- Houlihan JM., "The human amnion is a site of MHC class Ib expression : evidence for the expression of HLA-E and HLA-G", J Immunol, 154, pp.5665~5674 (1995)
- Kim JC., "The effects on inhibition of corneal neovascularization after human amniotic membrane transplantation in severely damaged rabbit corneas", Korean J Ophthalmol, 9, pp.32~46 (1995)
- Prabhasawat P., "Comparison of conjunctival autografts, amniotic membrane grafts, and primary closure for pterygium excision", Ophthalmology, 104, pp.974~985 (1997)
- Tseng SC., "Amniotic membrane transplantation with or without limbal allografts for corneal surface reconstruction in patients with limbal stem cell deficiency", Arch Ophthalmol, 116, pp.431~441 (1998)
- Lee SH., "Amniotic membrane transplantation for persistent epithelial defects with ulceration", Am J Ophthalmol, 123, pp.303~312 (1997)
- Mosmann T., "Rapid colorimetric assay for cellular growth and survival : application to proliferation and cytotoxicity assays", J Immunol Methods, 65, pp.55~63 (1983)
- Davis JW., "Skin transplantation with a review of 550 cases at the Johns Hopkins Hospital", Johns Hopkins Med J, 15, pp.307~ (1910)
- Meller D., "Ex vivo preservation and expansion of human limbal epithelial stem cells on amniotic membrane cultures", Br J Ophthalmol, 86, pp.463~471 (2002)
- Meller D., "Conjunctival epithelial cell differentiation on amniotic membrane", Invest Ophthalmol Vis Sci, 40, pp.878~886 (1999)
- Hao Y., "Identification of antiangiogenic and antiinflammatory proteins in human amniotic membrane", Cornea, 19, pp.348~352 (2000)
- van Herendael BJ., "Microanatomy of the human amniotic membranes. A light microscopic, transmission, and scanning electron microscopic study", Am J Obstet Gynecol, 131, pp.872~880 (1978)
- Dua HS., "Amniotic membrane transplantation", Br J Ophthalmol, 83, pp.748~752 (1999)
- Li DQ., "Differential expression and regulation of TGF-beta1, TGF-beta2, TGF-beta3, TGF-betaRI, TGF-betaRII and TGF-betaRIII in cultured human corneal, limbal, and conjunctival fibroblasts", Curr Eye Res, 19, pp.154~161 (1999)
- Tseng SC., "Suppression of transforming growth factor-beta isoforms, TGF-beta receptor type II, and myofibroblast differentiation in cultured human corneal and limbal fibroblasts by amniotic membrane matrix", J Cell Physiol, 179, pp.325~335 (1999)
- 이기환, "다양한 각막표면질환에서 양막이식술의 임상적 효과", 대한안과학회지, 48(1), pp.7~12 (2007), 대한안과학회
- 오두환, "심부 각막 궤양 및 천공 환자에서 시행한 다섯 겹 강화형 양막 이식술", 대한안과학회지, 52(10), pp.1232~1237 (2011), 대한안과학회
- 장지훈, "양막 이식을 이용한 군날개의 치료 효과", 대한안과학회지, 46(4), pp.597~604 (2005), 대한안과학회
- Lee DY., "The effect of amniotic membrane ointment application on photore fractive keratectomized cornea in rabbits", J Korean Ophthalmol Soc, 41, pp.2069~2077 (2000)
- Portera-Cailliau C., "Evidence for apoptotic cell death in Huntington disease and excitotoxic animal models", J Neurosci, 15(5 Pt 2), pp.3775~3787 (1995)
- Charriaut-Marlangue C., "Apoptotic features of selective neuronal death in ischemia, epilepsy and gp 120 toxicity", Trends Neurosci, 19, pp.109~114 (1996)
- Leist M., "Tumor necrosis factor-induced hepatocyte apoptosis precedes liver failure in experimental murine shock models", Am J Pathol, 146, pp.1220~1234 (1995)