군날개의 노화 관련 표지자 발현과 세포노화 확인
Cellular Senescence in Pterygium
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목적: 군날개에서 노화 관련 표지자의 발현을 확인하여 세포노화를 밝히고자 한다. 대상과 방법: 2019년 8월부터 2020년 1월까지 본원에서 군날개 혹은 검열반 진단을 받은 환자 28명 28안을 대상으로 impression cytology를 실시하여 얻은 검체에서 senescence-associated beta galactosidase (SA-β-gal)의 활성과, p16, interleukin-1β (IL-1β)의 발현 강도를 확인하였다. 결과: 전체 세포 수에 대한 SA-β-gal 양성인 세포수의 평균 백분율은 군날개군에서 67.63 ± 16.61%, 대조군에서 32.64 ± 8.98%로 군날개군에서 대조군에 비해 유의하게 높은 값을 나타냈다(p<0.01). IL-1β의 형광발현 강도는 군날개군에서 평균 22.53 ± 19.21로 평균 11.38 ± 6.30의 대조군에 비해 유의하게 높았으며(p=0.02), p16 형광발현에서도 군날개군과 대조군에서의 평균 발현 강도가 42.79 ± 23.65, 26.73 ± 18.34로 유의한 차이를 보였다(p=0.01). 결론: 군날개에서 노화세포에 특징적으로 발견되는 SA-β-gal, IL-1β, p16의 발현을 확인할 수 있었고 이는 군날개 발생과 진행에 있어 세포노화라는 새로운 기전의 정립에 도움이 될 것이라 기대한다.
Abstract
Purpose: To determine cellular senescence in pterygium by studying the expression of senescence-related markers. Methods: Impression cytology was performed on 28 eyes of 28 patients diagnosed with pterygium or pinguecula from August 2019 to January 2020. From the obtained specimen, the expression of senescence-associated beta galactosidase (SA-β-gal), p16, and interleukin (IL)-1β was examined. Results: The average percentage of SA-β-gal positive cells was significantly higher for the pterygium group (67.63 ± 16.61%) compared to the control group (32.64 ± 8.98%) (p < 0.01). The fluorescent expression intensity of IL-1β was higher in the pterygium group (22.53 ± 19.21) compared to the control group (11.38 ± 6.30) (p = 0.02), and the p16 fluorescence expression intensity was also higher in the pterygium group, showing 42.79 ± 23.65, compared to the control group values (26.73 ± 18.34) (p = 0.01). Conclusions: Cellular senescence specific markers, SA-β-gal, IL-1β, and p16, were expressed in pterygium, indicating a new possible framework for the development and progression of pterygium.
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