삼투압을 이용한 안구건조증 모델에서 예측 가능한 염증인자 분석
Inflammatory Factors Predicting Dry Eye Syndrome in a Model Using Osmotic Pressure
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목적: 고삼투압 안구건조증 모델을 이용하여 안구건조증에서 연구 표지자로 사용할 수 있는 염증인자(inflammatory factor)를 알아보고자 하였다. 대상과 방법: 고삼투압성 환경에 따른 세포 생존율 및 염증인자들을 평가하기 위해 배양 인체결막상피세포에 혈청 유무에 따라 400 mOsm/L, 450 mOsml/L, 500 mOsm/L, 550 mOsm/L 농도의 NaCl을 처리하여 세포생존성을 측정하였다. 또한 interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor (TNF)-α, metalloproteinase (MMP)-2, MMP-9에 대한 real-time polymerase chain reaction을시행하여 결과를 비교하였다. 결과: NaCl의 농도가 증가할수록 500 mOsm/L에서부터 세포 생존율이 유의하게 감소하였으며(p<0.05), NaCl의 농도에 비례하여IL-8, TNF-α, MMP-9이 유의하게 증가하였다(p<0.05). 결론: IL-8, TNF-α, MMP-9이 추후 안구건조증 연구에서 연구 표지자로 유용하게 사용할 수 있을 것으로 생각된다.
Abstract
Purpose: To identify inflammatory factors that may serve as biomarkers for dry eye syndrome using a hyperosmotic dry eye syndrome model. Methods: Cultured human conjunctival epithelial cells were subjected to 400, 450, 500, and 550 mOsm/L NaCl, and cell viability was assessed in response to osmolarity. The relative expression of inflammatory factors was evaluated by real-time polymerase chain reaction of interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor (TNF)-α, metalloproteinase (MMP)-2, and MMP-9 according to the NaCl concentration. Results: An increase in the concentration of NaCl led to a notable decrease in cell viability (p < 0.05). IL-8, TNF-α, and MMP-9 expression levels increased significantly in proportion to NaCl concentration (p < 0.05). Conclusions: IL-8, TNF-α, and MMP-9 may serve as effective biomarkers in dry eye studies.
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