흰쥐 콩팥발생과정에서 interleukin-6와그 수용체의 발현
The Expression of Interleukin-6 and Its Receptorin the Developing Rat Kidney
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Interleukin-6 (IL-6)와 그 수용체는 신경발생조직에서 중요한 기능을 담당하는 것으로 알려져 있으나, 콩팥의 발생과정에서의 기능은 거의 보고된 바가 없다. 본 연구에서는 IL-6와 그 특이수용체인 IL-6R의 발현을 흰쥐의 발생중인 콩팥에서 조사하였다. 임신 16, 18 및 20일 태아와 출생후 1, 3, 7, 14일의 동물 및 성체의 콩팥을 사용하여 IL-6와 그 수용체의 발현을 면역조직화학법과 in situ hybridization 법으로 각각 관찰하였다. IL-6 단백질은 임신 16일의 태아에서 이미 발현되었으며, 요관싹과 사이조직의 중간엽세포를 포함하여 콩팥단 위발생구역의 여러 발생단계의 콩팥단위 구조물에 강한 면역반응성을 보였다. 그러나 성숙된 사구체와 근위세관 에서는 발현되지 않았다. 출생후의 동물에서도 콩팥단위발생구역에서의 발현양상은 태아의 것과 같았으며, 성숙된 콩팥세관에서는 원위세관과 집합세관에 강한 면역반응성이, 굵은오름세관에 매우 미약한 면역반응성이 관찰되었 고, 이러한 양상은 성체에까지 계속되었다. IL-6R mRNA의 발현은 임신 16일의 태아에서 이미 관찰되며, 출생전 태아 콩팥에서의 발현부위는 IL-6의 것과 유사하였다. 출생후의 성숙된 콩팥단위에서의 IL-6R mRNA 발현은 원위 세관, 굵은오름세관 및 집합세관에서 관찰되었으며, 근위세관에서는 S3 부분에서만 발현되었다. 본 연구의 결과로서 IL-6가 그 수용체를 통하여 콩팥단위발생의 초기과정에 관련되어 역할을 담당하고 있는 것 으로 보여지며, 성숙된 콩팥에서도 IL-6 단백질과 그 수용체가 계속 발현되는 것으로 보아 일정한 기능을 담당할 것으로 생각된다.
Abstract
Interleukin-6 (IL-6) and its receptor are presumed to play important roles in the developing nervous system. However, little is known about their potential role(s) in the developing kidney. To investigate this, we have studied the expression of IL-6 and its receptor (IL-6R) in the developing rat kidney. Kidneys from 16- (F16), 18- and 20-day-old (F20) fetuses, 1- (P1), 3- (P3), 7- (P7) and 14-day-old (P14) pups, and adult rats were extracted. Renal expressions of IL-6 and its receptors were examined by immunohistochemistry and in situ hybridization respectively. Il-6 protein already appeared in F16. The early stage of renal development before birth, IL-6 showed strong immunoreactivity in the ureteric bud, metanephric mesenchymal cells (MMC) and developing glomerulus. The expression pattern of IL-6 in nephrogenic zone are very similar even after birth. In matured nephron after birth, IL-6 immunoreactivities were detected in distal tubules strongly, and collecting ducts moderately and thick ascending limb weekly. IL- 6R hybridization signals have also already appeared in 16-day old fetal kidney. Before birth, IL-6R mRNAs were expressed in ureteric bud, MMC and developing glomerulus. In the matured nephron after birth, IL-6R mRNA was expressed in the thick ascending limb, distal tubules, collecting ducts and S3 segment of proximal tubule. These results suggest that IL-6 and its receptor may be involved in regulation of nephron formation in nephrogenic zone of rat, and play a role in distal nephron including collecting duct after birth.
참고문헌 (21)
- Barasch J, "Mesenchymal to epithelial conversion in rat metanephros is induced by LIF", 99, pp.377~386 (1999)
- Dame JB, "The distribution of receptors for the proinflammatory cytokines interleukin (IL)-6 and IL-8 in the developing human fetus", 58, pp.25~39 (2000)
- Fukatsu A, "Distribution of interleukin-6in normal and diseasedhuman kidney", pp.61~66 (1991)
- Gadient RA, "Leukemia inhibitory factor, interleukin 6, and other cytokines using the gp130 transducing receptor: roles in inflammation and injury", 17, pp.127~137 (1999)
- Heinrich PC, "Interleukin-6-type cytokine signalling through the gp130", Jak, pp.1998, 334[DOI]
- Hirano T, "Signaling mechanisms through gp130: a model of the cytokine system", 8, pp.241~252 (1997)
- Homsi E, "Interleukin-6 stimulates tubular regeneration in rats with glycerol-induced acute renal failure", 92, pp.192~199 (2002)
- Horii Y, "Involvement of IL-6 in mesangial proliferative glomerulonephritis", iwa j immu(m l 143), pp.3949~3955 (1989)
- Jones SA, "IL-6 transsignaling: the in vivo consequences", 25, pp.241~253 (2005)
- Kayama F, "Cadmiuminduced renal damage and proinflammatory cytokines: possible role of IL-6 in tubular epithelial cell regeneration", 134, pp.26~34 (1995)
- Makela S, "Urinary excretion of interleukin-6 correlates with proteinuria in acute Puumala hantavirus-induced nephritis", 43, pp.809~816 (2004)
- M?rz P, "Neural activities of IL-6-type cytokines often depend on soluble cytokine receptors", 11, pp.2995~3004 (1999)
- Naka T, "The paradigm of IL-6: from basic science to medicine", 4, pp.S233~S242 (2002)
- Nigam SK, "Development and Maturation of the kidney", pp.72~98 (1996)
- Plisov SY, "TGFb2, LIF and FGF2 cooperate to induce nephrogenesis", 128, pp.1045~1957 (2001)
- Ranieri E, "Urinary IL- 6", EGF ratio: a useful prognostic marker for the progression of renal damage in IgA nephropathy, 1996, pp.50, 1990~2001
- Ruiz-Ortega M, "Angiotensin II regulates the synthesis of proinflammatory cytokines and chemokines in the kidney", 82, pp.12~22 (2002)
- Sadeghi M, "High urine sIL-6R as a predictor of late graft failure in renal transplant recipients", 76, pp.1190~1194 (2003)
- Saito M, "Molecularcloning of a murine IL-6 receptor-associated signaltransducer and its regulated expression in vivo", jimmu(l 148), pp.4066~4071 (1992)
- Suzuki H, "Serum soluble interleukin-6 receptorin MRL", lpr mice is elevated with age and mediates theinterleukin-6 signal, 1993, pp.eur j immu(l 23), 1078~1082
- Taniguchi Y, "Interleukin-6 Localization", 81, pp.94~98 (1999)