출생 전 스트레스와 감금 스트레스가 편도복합체에 미치는 영향: II. 별아교세포의 세포질돌기에 미치는 영향
Effects of Prenatal Stress and Restraint Stress on Amygdala Complex of the Rat: II. Effects on the Astrocytic Cell Processes
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편도복합체는 위험을 알리는 중요한 감각정보를 받고, 이러한 정보를 모아 공포라는 감정을 만들어낸다. 이와 같은 유해한 경험이 편도복합체 신경전달물질의 균형을 변화시키고 결국 스트레스 반응을 유도한다. 중추신 경계통에서 신경전달물질의 조절에 별아교세포가 관여하므로 본 연구에서는 출생 전 스트레스와 성장 후에 받은 스트레스가 편도복합체 별아교세포의 세포질돌기에 어떤 영향을 미치는지를 조사하였다. 이를 위해 흰쥐를 스트레스를 받지 않은 대조군, 성장 후 감금스트레스를 받은 군, 출생 전 스트레스를 받은 군, 출생 전 스트레스와 성장 후 감금스트레스를 모두 받은 군으로 구분하였다. 별아교세포는 GFAP 항체를 이용한 면역조직화학 염색을 시행하여 확인하였으며, methylene blue/azure II로 대조 염색하였다. Neurolucida 프로그램을 이용하여 별아교세포 돌기를 계측한 결과, 대부분의 편도복합체 신경핵에서 출생 전 스 트레스와 성장 후 감금스트레스를 모두 받은 군에서 한 개의 별아교세포에 분포하는 총 돌기의 수와 길이가 대조 군에 비해 유의하게 증가하였는데 이는 스트레스가 편도복합체에 분포하는 별아교세포의 형태적인 변화를 초래 하였음을 암시한다.
Abstract
The hypothesis that adverse experience alters the balance of neurotransmitters in the amygdala rendering it hyperresponsive to stress was examined in the present study. Since astrocytes regulate the brain’s neurochemical milieu by uptaking neurotransmitters, we have examined these cells in the amygdala of prenatally stressed rats, a model of pathological anxiety. Here we examined morphometric changes on the cell bodies of astrocytes in the amygdala induced by prenatal stress and restraint stress. For this purpose, rats were classified into 4 groups; control group (CON), only restraint stressed (starting on P90 for 3 days) group (CONR), prenatally stressed group (PNS), and prenatally and restraint (on P90 for 3 days) stressed group (PNSR). Astrocytes stained with GFAP immunohistochemistry were counter stained with methylene blue/azure II and were examined using the Neurolucida. The present study showed that prenatal and restraint stress caused the significant increase in the total number and length of the amygdaloid astrocytic processes. In conclusion, astrocytes show structural indices of activation with stress.
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