Immunohistochemical Study of O-GlcNAcylation in Human Skin Tumors
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초록
O-linked β-N-acetylglucosamine modification is an important post-translational modification,emerging as a novel regulatory mechanism in various cellular events. Recently, several studies have shown thatO-GlcNAcylation plays an essential role in human breast, lung, and colon cancers. With regard to skin cancers,the role of O-GlcNAcylation has yet to be elucidated. To investigate whether O-GlcNAcylation is linked tohuman skin tumor development, immunohistochemical analysis was performed to investigate the presence of OGlcNAcylationin various skin tumors. We evaluated the levels of O-GlcNAcylation, O-GlcNAc transferase, andO-GlcNAcase in 29 benign tumors, 12 premalignant tumors, and 26 malignant tumors in skin. Compared to thebenign tumors, premalignant and malignant tumors had increased patterns of O-GlcNAcylation. In addition, theO-GlcNAc transferase and O-GlcNAcase levels were higher in premalignant and malignant tumors than in benigntumors. Interestingly, O-GlcNAcase levels were significantly increased in premalignant tumors compared tobenign and malignant tumors. These results suggest that O-GlcNAcylation of proteins may play an important rolein the development of human skin tumors.
Abstract
O-linked β-N-acetylglucosamine modification is an important post-translational modification,emerging as a novel regulatory mechanism in various cellular events. Recently, several studies have shown thatO-GlcNAcylation plays an essential role in human breast, lung, and colon cancers. With regard to skin cancers,the role of O-GlcNAcylation has yet to be elucidated. To investigate whether O-GlcNAcylation is linked tohuman skin tumor development, immunohistochemical analysis was performed to investigate the presence of OGlcNAcylationin various skin tumors. We evaluated the levels of O-GlcNAcylation, O-GlcNAc transferase, andO-GlcNAcase in 29 benign tumors, 12 premalignant tumors, and 26 malignant tumors in skin. Compared to thebenign tumors, premalignant and malignant tumors had increased patterns of O-GlcNAcylation. In addition, theO-GlcNAc transferase and O-GlcNAcase levels were higher in premalignant and malignant tumors than in benigntumors. Interestingly, O-GlcNAcase levels were significantly increased in premalignant tumors compared tobenign and malignant tumors. These results suggest that O-GlcNAcylation of proteins may play an important rolein the development of human skin tumors.
참고문헌 (18)
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