Compound 48/80에 의한 비만세포 활성화와 IgE에 의한 혈관투과성 증가에 대한 웅담의 억제효과
Inhibitory Effect of Bear Bile on Compound 48/80-induced Mast Cell Activation and IgE-mediated Vascular Permeability
0
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
비만세포는 알레르기 질환의 병태생리에 중요한 역할을 한다. 웅담이 compound 48/80에 의한 비만세포 의 활성화와 IgE에 의한 혈관투과성 증가를 억제하는지 알아보고, 그 약리기전을 구명하기 위하여 이 연구를 실 시하였다. 시험관 내 실험에서 웅담이 compound 48/80에 의한 비만세포의 탈과립과 히스타민 유리, 비만세포 속으 로의 칼슘유입, 비만세포 내의 cAMP 수준에 미치는 영향을 관찰하였다. 또한 생체 실험에서 웅담을 입안으로 투 여한 다음 IgE에 의한 피부 혈관투과성 증가에 미치는 영향을 관찰하였다. 웅담 전처리에 의하여 compound 48/80 에 의한 흰쥐 복강 비만세포의 탈과립과 비만세포로부터의 히스타민 유리, 비만세포 내로의 칼슘유입이 억제되었 다. 복강 비만세포 내 cAMP 수준도 웅담에 의해 증가되었다. 또한 IgE에 의한 피부 혈관투과성 증가가 웅담의 전 처리로 억제되었다. 이상의 결과로 미루어, 웅담은 compound 48/80에 의한 비만세포 활성화와 IgE에 의한 혈관투 과성 증가를 억제하는 항알레르기성 물질을 함유하고 있다고 생각된다. 웅담은 비만세포 활성화로 야기되는 알레 르기 질환의 치료제로 이용할 수 있을 것으로 기대된다.
Abstract
The bear bile has been used as a traditional drug medicine and has been known to have anti-tumor, anti-inflammatory and anti-oxidant effects. The purpose of this study is to investigate the inhibitory effect of bear bile on compound 48/80-induced mast cell activation in vitro and anti-dinitrophenyl (DNP) IgE-mediated vascular permeability in vivo. For this, the effects of bear bile on the degranulation, histamine release, calcium influx and the change of the intracellular cAMP levels of rat peritoneal mast cells (RPMCs) and the influences of the oral treatment of bear bile on IgE-mediated cutaneous vascular permeability were studied. the results were as follows; the compound 48/80-induced degranulation, histamine release and calcium influx of RPMCs were inhibited by pretreatment with bear bile, the cAMP levels of RPMCs were increased by pretreatment with bear bile, and bear bile inhibited anti-DNP IgE-mediated cutaneous vascular permeability. From the above results, it is suggested that bear bile contains some substances which inhibit anti-DNP IgE-mediated vascular permeability and mast cell activation. Bear bile potentially may serve as an effective therapeutic agent for allergic diseases.
참고문헌 (36)
- Caufield JP, "Secretion in dissociated human pulmonary mast cells. Evidence for solubilization of granule contents before discharge", J Cell Biol, 85, pp.29~37 (1980)
- Chai OH, "Inhibitory effect of Cortex mori on the colchicine-induced mast cell activation", Korean J Anat, 32, pp.735~747 (1999)
- Chai OH, "Inhibitory effects of Morus alba on compound 48", 80-induced anaphylactic reactions and anti-chicken gamma globulin IgEmediated mast cell activation, 2005, pp.28, 1852~1858
- Chai OH, "Effect of Cortex mori on spermine- induced histamine release, changes of cAMP and cGMP levels of rat peritoneal mast cells", Korean J Immunol, 18, pp.353~365 (1996)
- Chang L, "Cytoskeleton of living, unstained cells imaged by scanning force microscopy", Biophys J, 6, pp.1282~1286 (1993)
- Choi YH, "Inhibition of anaphylaxis-like reaction and mast cell activation by water extract from the fruiting body of Phellinus linteus", Biol Pharm Bull, 29, pp.1360~1365 (2006)
- Choi YH, "Lee MS, Han EH, Kim HT, Song CH : Inhibitory effects of Agaricus blazei on mast cell-mediated anaphylaxis-like reactions", Biol Pharm Bull, 29, pp.1366~1371 (2006)
- Ehrlich P, "Beitrage zur kenntnis der Anilinfarbunger und ihrer Verwendung in dermikroskopischen Technik", Arch Mikrosk Anst, 13, pp.263~277 (1877)
- Galli SJ, "New concepts about the mast cell", N Engl J Med, 28, pp.257~265 (1993)
- Ganong WF, "Review of Medical Physiology, 22nd ed", pp.39~41 (2005), The McGraw-Hill Companies
- Gauthier P, "Protection against histamine bronchospasm and Herxheimer anaphylactic microshock using a bile extract", Union Med Can, 103, pp.426~431 (1974)
- Hachisuka H, "Purification of rat cutaneous mast cells with Percoll density centrifugation", Arch Dermatol Res, 280, pp.358~362 (1988)
- Harvima RJ, "Optimization of histamine radioenzymatic assay with purified histamine N-methyltransferase", Clinica Chimica Acta, 171, pp.247~256 (1988), Fraki JE:Optimization of histamine radioenzymatic assay with purified histamine N-methyltransferase.Clinica Chimica Acta 171:247-256
- Holmegaard SN, "Measurement of cAMP in clinical investigations", Acta Endocrinologica, 101, pp.1~46 (1982)
- 김욱, "암세포주의 p53 단백질 발현에 대한 웅담의 영향", Anatomy and Cell Biology, 38(1), pp.49~54 (2005), 대한해부학회
- Kang KJ, "Inhibitory effect of Mori cortex on compound 48", 80-induced histamine release and calcium uptake of rat peritoneal mast cells, 1993, pp.15, 91~99
- 김은경, "우방자 추출물의 compound 48", 80 유도 비만세포 활성화와 혈관 투과성 억제효과, 2004, pp.17(1), 55~66[DOI]
- Kim SH, "Action of Dracocephalum argunense on mast cell-mediated allergy model", Biol Pharm Bull, 29, pp.494~498 (2006)
- Kubo M, "Studies on medicinal resources from livestock. I. Antiallergic effects of pig bile (1)", Chem Pharm Bull, 37, pp.3409~3411 (1989)
- Lee MS, "Inhibitory effect of polysaccharide fraction from Cortex mori on compound 48", 80-induced mast cell activation, 1999, pp.21, 35~45
- Guang Zhao Li, "Inhibitory effects of epigallocatechin gallate on compound 48", 80-induced mast cell activation and passive cutaneous anaphylaxis, 2005, pp.37(4), 290~296[DOI]
- Li GZ, "Inhibitory effects of Houttuynia cordata water extracts on anaphylactic reaction and mast cell activation", Biol Pharm Bull, 28, pp.1864~1868 (2005)
- 임정민, "산수유 추출물의 compund 48", 80에 의한 비만세포 활성화와 혈관 투과성 억제효과, 2004, pp.17(1), 19~30[DOI]
- Masini E, "Helicobacter pylori potentiates histamine release from rat serosal mast cells induced by bile acids", Dig Dis Sci, 39, pp.1493~1500 (1994), Sacchi TB:Helicobacter pylori potentiates histamine release from rat serosal mast cells induced by bile acids.Dig Dis Sci 39:1493-1500
- Morita K, "A desmutagenic factor isolated from burdock (Arctium lappa Linne)", Mutat Res, 129, pp.25~31 (1984)
- Nakagami T, "Studies on medicinal resources from livestock. II. Anti-allergic effects of pig bile (2)", Chem Pharm Bull, 38, pp.706~708 (1990)
- Nillsson G, "Mast cell and basophil, in Inflammation : Basic principles and clinical correlates, 3rd ed", pp.97~117 (1999), Lippincott Williams & Wilkins
- Park JE, "Effect of colchicine on rat peritoneal mast cells pretreated Cortex mori", Korean J Anat, 20, pp.358~364 (1987)
- Pelletier G, "Natural antiallergenic factors in bile", Ann Immunol, 128, pp.261~263 (1977)
- Petersen LJ, "Allergen-induced histamine release in intact human skin in vivo assessed by skin microdialysis technique : characterization of factors influencing histamine releasability", J Allergy Clin Immunol, 97, pp.672~683 (1996)
- Quist RG, "Activation of mast cells by bile acids", Gastroenterology, 101, pp.446~456 (1991)
- Richard AG, "Kuby Immunology, 4th ed.", pp.401~404 (1999), W.H. Freeman and Company
- Roitt I, "Immunology, 6th ed", pp.323~343 (2001), Gower Medical Publishing Ltd
- Sieghart W, "Phosphorylation of a single mast cell protein in response to drugs that inhibit secretion", Biochem Pharmacol, 30, pp.2737~2742 (1981)
- Tasaka K, "Role of the cytoskeletons on Ca2+ release from the intracellular Ca2+ store of rat peritoneal mast cell", Skin Pharmacol, 4, pp.43~52 (1991)
- Yoshii N, "Role of endoplasmic reticulum, an intracellular Ca2+ store, in histamine release from rat peritoneal mast cell", Immunopharmacol, 21, pp.13~21 (1991)