중증 천식 및 만성폐쇄성폐질환 환자에서 IgG 자가항체가 인지하는 기도상피세포 자가항원 단백질 분석
Analysis of Airway Epithelial Cell Autoantigens Recognized by IgG Autoantibodies from Patients with Severe Asthma and Chronic Obstructive Pulmonary Disease
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Background: The presence of circulating autoantibodies to airway epithelial cells has been reported in patients with asthma and chronic obstructive pulmonary disease (COPD). Objective: To evaluate the possibility of a common autoantigen associated with both asthma and COPD, we analyzed airway epithelial cell autoantigens recognized by circulating IgG autoantibodies from patients with severe asthma and COPD. Method: IgG autoantibodies to human airway epithelial cells (A549) were examined in serum samples from patients with severe asthma and COPD by immunoblot analysis. Result: A 54-kDa airway epithelial autoantigen was commonly recognized by circulating IgG antibodies from patients with severe asthma and COPD. The 54-kDa auto-antigen was identified as cytokeratin 8 protein by mass spectrometry. IgG autoantibodies to recombinant human cytokeratin 8 protein were detected in serum samples from 5 (31.3%) of the 16 patients with severe asthma, 4 (26.6%) of 15 patients with COPD, 1 (7.7%) of 13 patients with mild-to-moderate asthma, and 1 (7.7%) of 13 healthy controls (P=0.056). Conclusion: In this study, we identified cytokeratin 8 as an airway epithelial autoantigen associated with both severe asthma and COPD. Further studies are needed to determine the role of this autoantigen in the pathogenesis of severe asthma and COPD.
Abstract
Background: The presence of circulating autoantibodies to airway epithelial cells has been reported in patients with asthma and chronic obstructive pulmonary disease (COPD). Objective: To evaluate the possibility of a common autoantigen associated with both asthma and COPD, we analyzed airway epithelial cell autoantigens recognized by circulating IgG autoantibodies from patients with severe asthma and COPD. Method: IgG autoantibodies to human airway epithelial cells (A549) were examined in serum samples from patients with severe asthma and COPD by immunoblot analysis. Result: A 54-kDa airway epithelial autoantigen was commonly recognized by circulating IgG antibodies from patients with severe asthma and COPD. The 54-kDa auto-antigen was identified as cytokeratin 8 protein by mass spectrometry. IgG autoantibodies to recombinant human cytokeratin 8 protein were detected in serum samples from 5 (31.3%) of the 16 patients with severe asthma, 4 (26.6%) of 15 patients with COPD, 1 (7.7%) of 13 patients with mild-to-moderate asthma, and 1 (7.7%) of 13 healthy controls (P=0.056). Conclusion: In this study, we identified cytokeratin 8 as an airway epithelial autoantigen associated with both severe asthma and COPD. Further studies are needed to determine the role of this autoantigen in the pathogenesis of severe asthma and COPD.
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