Effect of Catechins, Green tea Extract and Methylxanthines in Combination with Gentamicin Against Staphylococcus aureus and Pseudomonas aeruginosa - Combination therapy against resistant bacteria -
5
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
Objectives: Bacterial resistant infections have become a global health challenge and threaten the society’s health. Thus, an urgent need exists to find ways to combat resistant pathogens. One promising approach to overcoming bacterial resistance is the use of herbal products. Green tea catechins, the major green tea polyphenols, show antimicrobial activity against resistant pathogens. The present study aimed to investigate the effect of catechins, green tea extract, and methylxanthines in combination with gentamicin against standard and clinical isolates of Staphylococcus aureus (S. aureus) and the standard strain of Pseudomonas aeruginosa (P. aeruginosa). Methods: The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) values of different agents against bacterial strains were determined. The interactions of green tea extract, epigallate catechin, epigallocatechin gallate, two types of methylxanthine, caffeine, and theophylline with gentamicin were studied in vitro by using a checkerboard method and calculating the fraction inhibitory concentration index (FICI). Results: The MICs of gentamicin against bacterial strains were in the range of 0.312 - 320 μg/mL. The MIC values of both types of catechins were 62.5 - 250 μg/ mL. Green tea extract showed insufficient antibacterial activity when used alone. Methylxanthines had no intrinsic inhibitory activity against any of the bacterial strains tested. When green tea extract and catechins were combined with gentamicin, the MIC values of gentamicin against the standard strains and a clinical isolate were reduced, and synergistic activities were observed (FICI < 1). A combination of caffeine with gentamicin did not alter the MIC values of gentamicin. Conclusion: The results of the present study revealed that green tea extract and catechins potentiated the antimicrobial action of gentamicin against some clinical isolates of S. aureus and standard P. aeruginosa strains. Therefore, combinations of gentamicin with these natural compounds might be a promising approach to combat microbial resistance.
Abstract
Objectives: Bacterial resistant infections have become a global health challenge and threaten the society’s health. Thus, an urgent need exists to find ways to combat resistant pathogens. One promising approach to overcoming bacterial resistance is the use of herbal products. Green tea catechins, the major green tea polyphenols, show antimicrobial activity against resistant pathogens. The present study aimed to investigate the effect of catechins, green tea extract, and methylxanthines in combination with gentamicin against standard and clinical isolates of Staphylococcus aureus (S. aureus) and the standard strain of Pseudomonas aeruginosa (P. aeruginosa). Methods: The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) values of different agents against bacterial strains were determined. The interactions of green tea extract, epigallate catechin, epigallocatechin gallate, two types of methylxanthine, caffeine, and theophylline with gentamicin were studied in vitro by using a checkerboard method and calculating the fraction inhibitory concentration index (FICI). Results: The MICs of gentamicin against bacterial strains were in the range of 0.312 - 320 μg/mL. The MIC values of both types of catechins were 62.5 - 250 μg/ mL. Green tea extract showed insufficient antibacterial activity when used alone. Methylxanthines had no intrinsic inhibitory activity against any of the bacterial strains tested. When green tea extract and catechins were combined with gentamicin, the MIC values of gentamicin against the standard strains and a clinical isolate were reduced, and synergistic activities were observed (FICI < 1). A combination of caffeine with gentamicin did not alter the MIC values of gentamicin. Conclusion: The results of the present study revealed that green tea extract and catechins potentiated the antimicrobial action of gentamicin against some clinical isolates of S. aureus and standard P. aeruginosa strains. Therefore, combinations of gentamicin with these natural compounds might be a promising approach to combat microbial resistance.
참고문헌 (30)
- Khameneh B, "Breakthroughs in bacterial resistance mechanisms and the potential ways to combat them", Microb Pathog, 95, pp.32~42 (2016)
- Khademi F, "Helicobacter pylori in Iran: a systematic review on the antibiotic resistance", Iran J Basic Med Sci, 18(1), pp.2~7 (2015)
- Riley MA, "Resistance is futile: The bacteriocin model for addressing the antibiotic resistance challenge", Biochem Soc Trans, 40(6), pp.1438~1442 (2012)
- Pantosti A, "Mechanisms of antibiotic resistance in Staphylococcus aureus", Future Microbiol, 2(3), pp.323~334 (2007)
- Corvec S, "Activities of fosfomycin, tigecycline, colistin, and gentamicin against extended-spectrum-lactamase-producing Escherichia coli in a foreign-body infection model", Antimicrob Agents Chemother, 57(3), pp.1421~1427 (2013)
- Forouzanfar F, "Black cumin (Nigella sativa) and its constituent (thymoquinone): a review on antimicrobial effects", Iran J Basic Med Sci, 17(12), pp.929~938 (2014)
- Khameneh B, "Combination of anti-tuberculosis drugs with vitamin C or NAC against different Staphylococcus aureus and mycobacterium tuberculosis strains", Microb Pathog, 93, pp.83~87 (2016)
- Hagihara M, "The efficacy and safety of antibiotic combination therapy for infections caused by gram-positive and gram-negative organisms", Expert Opin Drug Saf, 11(2), pp.221~233 (2012)
- Olosunde OF, "Phytochemical screening and antimicrobial properties of a common brand of black tea (Camellia sinensis) marketed in Nigerian environment", Adv Pharm Bull, 2(2), pp.259~263 (2012)
- Hamilton-Miller JM, "Antimicrobial properties of tea (Camellia sinensis L.)", Antimicrob Agents Chemother, 39(11), pp.2375~2377 (1995)
- Yam TS, "Microbiological activity of whole and fractionated crude extracts of tea (Camellia sinensis), and of tea components", FEMS Microbiol Lett, 152(1), pp.169~174 (1997)
- Chan EW, "Antioxidant and antibacterial properties of green, black, and herbal teas of Camellia sinensis", Pharmacognosy Res, 3(4), pp.266~272 (2011)
- Betts JW, "In vitro activity of curcumin in combination with epigallocatechin gallate (EGCG) versus multidrug-resistant Acinetobacter baumannii", BMC Microbiol, 14, pp.172~ (2014)
- Sharma A, "Green tea extract : possible mechanism and antibacterial activity on skin pathogens", Food Chem, 135(2), pp.672~675 (2012)
- on bacterial pathogens and indigenous bacterial probiotics of Western Himalayas, "Braz J Microbiol", 2013, 709~715
- Osterburg A, "Highly antibiotic-resistant acinetobacter baumannii clinical isolates are killed by the green tea polyphenol(-)-epigallocatechin-3-gallate(EGCG)", Clin Microbiol Infect, 15(4), pp.341~346 (2009)
- Zhao WH, "Inhibition of penicillinase by epigallocatechin gallate resulting in restoration of antibacterial activity of penicillin against penicillinase-producing Staphylococcus aureus", Antimicrob Agents Chemother, 46(7), pp.2266~2268 (2002)
- Elgaher WA, "Synthesis, anti-bronchoconstrictive, and antibacterial activities of some new 8-substituted-1, 3-dimethylxanthine derivatives", Bull Pharm Sci, 32(1), pp.153~187 (2009)
- Alaa M. Hayallah, "Design and Synthesis of Some New Theophylline Derivatives with Bronchodilator and Antibacterial Activities", Archives of Pharmacal Research, 34(1), pp.3~21 (2011), 대한약학회
- Bazzaz BS, "Interaction of methylxanthines and gentamicin against Staphylococcus aureus and Pseudomonas aeruginosa: role of phosphodiesterase inhibition", Acta Microbiol Immunol Hung, 59(1), pp.13~20 (2012)
- Hosseinzadeh H, "In vitro evaluation of methylxanthines and some antibiotics: interaction against Staphylococcus aureus and Pseudomonas aeruginosa", Iran Biomed J, 10(3), pp.163~167 (2006)
- Gyawali R, "Behavior and changes in cell morphology of Escherichia coli O157:H7 in liquid medium and skim milk in the presence of caffeine", CYTA-J Food, 12(3), pp.235~241 (2014)
- 김용완, "Antimicrobial and Antifungal Effects of Green Tea Extracts against Microorganisms Causing Vaginitis", Food Science and Biotechnology, 22(3), pp.713~719 (2013), 한국식품과학회[DOI]
- Khameneh B, "Investigation of the antibacterial activity and efflux pump inhibitory effect of co-loaded piperine and gentamicin nanoliposomes in methicillin-resistant Staphylococcus aureus", Drug Dev Ind Pharm, 41(6), pp.989~994 (2015)
- Anita P, "In vitro antibacterial activity of Camellia sinensis extract against cariogenic microorganisms", J Basic Clin Pharm, 6(1), pp.35~39 (2014)
- Toda M, "Antibacterial and bactericidal activities of Japanese green tea", Nihon Saikingaku Zasshi, 44(4), pp.669~672 (1989)
- Lee JH, "In vitro anti-adhesive activity of green tea extract against pathogen adhesion", Phytother Res, 23(4), pp.460~466 (2009)
- Chung JH, "Dual mechanisms of green tea extract (EGCG)-induced cell survival in human epidermal keratinocytes", FASEB J, 17(13), pp.1913~1915 (2003)
- Taylor PW, "Antimicrobial properties of green tea catechins", Food Sci Technol Bull, 2, pp.71~81 (2005)
- Rahal JJ Jr, "Antibiotic combinations: the clinical relevance of synergy and antagonism", Medicine (Baltimore), 57(2), pp.179~195 (1978)