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Enhancement of HIV-1 Tat fusion protein transduction efficiency by bog blueberry anthocyanins

조성우|김덕수|최수영|박진서|김대원|Mi Jin Kim|이길수|임순성|강태천|강일준|Eun Jeong Sohn|음원식|이선화|Hoon Jae Jeong
생화학분자생물학회 (Korean Society Of Biochemistry And Molecular Biology)|2010.08|BMB Reports|제43권 제8호|pp. 561-566

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참고문헌

키워드

Protein therapyBog blueberry anthocyaninsProtein transduction efficiencyTat fusion protein
주제분류화학

초록

Though protein transduction domains (PTDs) are well known for the delivery of exogenous therapeutic proteins into living cells, the overall low efficiency of transduction is a serious obstacle. We investigated the effect of bog blueberry anthocyanins (BBA) on protein transduction efficiency and found that BBA enhanced the transduction efficiencies of Tat-SOD fusion protein into HeLa cells and mice skin. The enzymatic activities in the cells and skin tissue in the presence of BBA were markedly increased compared to controls. Further, BBA did not demonstrate any cell toxicity at various concentrations. Although the mechanism is not fully understood, we suggest that BBA might alter the conformation of the membrane, which would indicate that BBA can be used as a protein transduction enhancer for the efficient delivery of therapeutic proteins for a variety of disorders.

Abstract

Though protein transduction domains (PTDs) are well known for the delivery of exogenous therapeutic proteins into living cells, the overall low efficiency of transduction is a serious obstacle. We investigated the effect of bog blueberry anthocyanins (BBA) on protein transduction efficiency and found that BBA enhanced the transduction efficiencies of Tat-SOD fusion protein into HeLa cells and mice skin. The enzymatic activities in the cells and skin tissue in the presence of BBA were markedly increased compared to controls. Further, BBA did not demonstrate any cell toxicity at various concentrations. Although the mechanism is not fully understood, we suggest that BBA might alter the conformation of the membrane, which would indicate that BBA can be used as a protein transduction enhancer for the efficient delivery of therapeutic proteins for a variety of disorders.

참고문헌 (42)

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