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CopA3 peptide from Copris tripartitus induces apoptosis in human leukemia cells via a caspase-independent pathway

김호|황재삼|Seong-Ryul Kim|Eun-Young Yun|Mi-Young Ahn|장종수|Sung-Hee Nam|Bo Ram Kang
생화학분자생물학회 (Korean Society Of Biochemistry And Molecular Biology)|2012.02|BMB Reports|제45권 제2호|pp. 85-90

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키워드

ApoptosisLeukemiaAnti-cancer effectCaspase-independent apoptosis pathwaysCell membrane penetrationInsect peptide
주제분류화학

초록

Our previous study demonstrated that CopA3, a disulfide dimer of the coprisin peptide analogue (LLCIALRKK), has antibacterial activity. In this study, we assessed whether CopA3caused cellular toxicity in various mammalian cell lines. CopA3 selectively caused a marked decrease in cell viability in Jurkat T, U937, and AML-2 cells (human leukemia cells),but was not cytotoxic to Caki or Hela cells. Fragmentation of DNA, a marker of apoptosis, was also confirmed in the leukemia cell lines, but not in the other cells. CopA3-induced apoptosis in leukemia cells was mediated by apoptosis inducing factor (AIF), indicating induction of a caspase-independent signaling pathway. [BMB reports 2012; 45(2): 85-90]

Abstract

Our previous study demonstrated that CopA3, a disulfide dimer of the coprisin peptide analogue (LLCIALRKK), has antibacterial activity. In this study, we assessed whether CopA3caused cellular toxicity in various mammalian cell lines. CopA3 selectively caused a marked decrease in cell viability in Jurkat T, U937, and AML-2 cells (human leukemia cells),but was not cytotoxic to Caki or Hela cells. Fragmentation of DNA, a marker of apoptosis, was also confirmed in the leukemia cell lines, but not in the other cells. CopA3-induced apoptosis in leukemia cells was mediated by apoptosis inducing factor (AIF), indicating induction of a caspase-independent signaling pathway. [BMB reports 2012; 45(2): 85-90]

참고문헌 (23)

  1. 인준교, "Isolation and characterization of a Glutamate decarboxylase (GAD) gene and their differential expression in response to abiotic stresses from Panax ginseng C. A. Meyer", Molecular Biology Reports, pp., 0(0), pp.1~9 (2009), Kluwer Academic Publishers
  2. Lamberty, M., "Insect immunity. Isolation from the lepidopteran Heliothis virescens of a novel insect defensin with potent antifungal activity", J. Biol. Chem., 274, pp.9320~9326 (1999)
  3. Varkey, J., "Antibacterial activity of human neutrophil defensin HNP-1 analogs without cysteines", Antimicrob. Agents Chemother., 49, pp.4561~4566 (2005)
  4. Raghuraman, H., "Cholesterol inhibits the lytic activity of melittin in erythrocytes", Chem. Phys. Lipids., 134, pp.183~189 (2005)
  5. Wieprecht, T., "Binding of the antibacterial peptide magainin 2 amide to small and large unilamellar vesicles", Biophys Chem., 85, pp.187~198 (2000)
  6. Zhang, W., "Role of AIF in human coronary artery endothelial cell apoptosis", Am. J. Physiol. Heart. Circ. Physiol., 286, pp.H354~H358 (2004)
  7. Kim, H., "Inflammation and apoptosis in Clostridium difficileenteritis is mediated by PGE2 up-regulation of Fas ligand", Gastroenterology, 133, pp.875~886 (2007)
  8. Okada, H., "Pathways of apoptotic and non-apoptotic death in tumour cells", Nat. Rev. Cancer, 4, pp.592~603 (2004)
  9. Zwaal, R. F., "Surface exposure of phosphatidylserine in pathological cells", Cell. Mol. Life. Sci., 62, pp.971~988 (2005)
  10. Ji-Hae Kim, "Induction of caspase-dependent apoptosis in melanoma cells by the synthetic compound (E)-1-(3,4-dihydroxyphenethyl)-3- styrylurea", BMB reports, 42(12), pp.806~811 (2009), 한국생화학분자생물학회
  11. Varkey, J., "Antibacterial activity of linear peptides spanning the carboxy-terminal beta-sheet domain of arthropod defensins", Peptides, 27, pp.2614~2623 (2006)
  12. Raghuraman, H., "Melittin: a membrane-active peptide with diverse functions", Biosci. Rep., 27, pp.189~223 (2007)
  13. Hancock, R. E., "The role of antimicrobial peptides in animal defenses", Proc. Natl. Acad. Sci. U.S.A., 97, pp.8856~8861 (2000)
  14. Dathe, M., "Structural features of helical antimicrobial peptides: their potential to modulate activity on model membranes and biological cells", Biochim. Biophys. Acta., 1462, pp.71~87 (1999)
  15. Iwasaki, T., "Selective cancer cell cytotoxicity of enantiomeric 9-mer peptides derived from beetle defensins depends on negatively charged phosphatidylserine on the cell surface", Peptides, 30, pp.660~668 (2009)
  16. Dong-Hyeon Shin, "ZAS3 promotes TNFα-induced apoptosis by blocking NFκB-activated expression of the anti-apoptotic genes TRAF1 and TRAF2", BMB Reports, 44(4), pp.267~272 (2011), 생화학분자생물학회
  17. Vilcek, J., "Tumor necrosis factor. New insights into the molecular mechanisms of its multiple actions", J. Biol. Chem., 266, pp.7313~7316 (1991)
  18. Malleo, G., "Role of tumor necrosis factor-alpha in acute pancreatitis: from biological basis to clinical evidence", Shock, 28, pp.130~140 (2007)
  19. Frolkis, I., "Interaction between paracrine tumor necrosis factor-alpha and paracrine angiotensin II during myocardial ischemia", J. Am. Coll. Cardiol., 37, pp.316~322 (2001)
  20. Kitanaka, C., "Ras protein expression and autophagic tumor cell death in neuroblastoma", Am. J. Surg. Pathol., 31, pp.153~155 (2007)
  21. Zeuner, A., "Control of erythroid cell production via caspase-mediated cleavage of transcription factor SCL", Tal-1, 2003, pp.10, 905~913
  22. Kim, H., "Clostridium difficile toxin A-induced colonocyte apoptosis involves p53-dependent p21(WAF1", CIP1) induction via p38 mitogen- activated protein kinase, 2005, pp.129, 1875~1888
  23. Tas, J., "Fundamental aspects of the interaction of propidium diiodide with nuclei acids studied in a model system of polyacrylamide films", J. Histochem. Cytochem., 29, pp.929~936 (1981)

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