Mesodermal repression of single-minded in Drosophila embryo is mediated by a cluster of Snail-binding sites proximal to the early promoter
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
single-minded (sim) is a master regulatory gene that directs differentiation in the central nervous system during Drosophila embryogenesis. Recent identification of the mesectoderm enhancer (MSE) of sim has led to the hypothesis that two Snail (Sna)-binding sites in the MSE may repress sim expression in the presumptive mesoderm. We provide evidence here that three Sna-binding sites proximal to the sim promoter, but not those of the MSE, are responsible for the mesodermal repression of sim in vivo. Using transgenic embryos injected with lacZ transgenes, we showed that sim repression in the mesoderm requires the three promoter-proximal Sna-binding sites. These results suggest that Sna represses the mesectodermal expression of sim by directly repressing the nearby promoter,and not by quenching adjacent transcriptional activators in the MSE. These data also showed how the MSE, lacking the three proximal Sna-binding sites, reproduced the endogenous pattern of sim expression in transgenic embryos. [BMB Reports 2012; 45(10): 577-582]
Abstract
single-minded (sim) is a master regulatory gene that directs differentiation in the central nervous system during Drosophila embryogenesis. Recent identification of the mesectoderm enhancer (MSE) of sim has led to the hypothesis that two Snail (Sna)-binding sites in the MSE may repress sim expression in the presumptive mesoderm. We provide evidence here that three Sna-binding sites proximal to the sim promoter, but not those of the MSE, are responsible for the mesodermal repression of sim in vivo. Using transgenic embryos injected with lacZ transgenes, we showed that sim repression in the mesoderm requires the three promoter-proximal Sna-binding sites. These results suggest that Sna represses the mesectodermal expression of sim by directly repressing the nearby promoter,and not by quenching adjacent transcriptional activators in the MSE. These data also showed how the MSE, lacking the three proximal Sna-binding sites, reproduced the endogenous pattern of sim expression in transgenic embryos. [BMB Reports 2012; 45(10): 577-582]
참고문헌 (25)
- Thomas, J. B., "Molecular genetics of the single-minded locus: a gene involved in the development of the Drosophila nervous system", Cell, 52, pp.133~141 (1988)
- Crews, S. T., "The Drosophila single-minded gene encodes a nuclear protein with sequence similarity to the per gene product", Cell, 52, pp.143~151 (1988)
- Nambu, J. R., "The single-minded gene of Drosophila is requiredfor the expression of genes important for the developmentof CNS midline cells", Cell, 63, pp.63~75 (1990)
- Nambu, J. R., "The Drosophila single-minded gene encodes a helix-loop-helix protein that acts as a master regulator of CNS midline development", Cell, 67, pp.1157~1167 (1991)
- Golembo, M., "The Drosophila embryonic midline is the site of Spitz processing, and induces activation of the EGF receptor in the ventral ectoderm", Development, 122, pp.3363~3370 (1996)
- Skeath, J. B., "The Drosophila EGF receptor controls the formation and specification of neuroblasts along the dorsal-ventral axis of the Drosophila embryo", Development, 125, pp.3301~3312 (1998)
- Arendt, D., "Comparison of early nerve cord development in insects and vertebrates", Development, 126, pp.2309~2325 (1999)
- Sonnenfeld, M., "The Drosophila tango gene encodes a bHLH-PAS protein that is orthologous to mammalian Arnt and controls CNS midline and tracheal development", Development, 124, pp.4571~4582 (1997)
- Wharton, K. A. Jr., "Control of CNS midline transcription by asymmetricE-box-like elements: similarity to xenobiotic responsiveregulation", Development, 120, pp.3563~3569 (1994)
- Hong, J. W., "How the Dorsal gradient works: insights from postgenome technologies", Proc. Natl. Acad. Sci. U.S.A., 105, pp.20072~20076 (2008)
- Reeves, G. T., "Graded dorsal and differential gene regulation in the Drosophila embryo", Cold Spring Harb. Perspect. Biol., 1, pp.a000836~ (2009)
- Kasai, Y., "Dorsal-ventral patterning in Drosophila: DNAbinding of snail protein to the single-minded gene", Proc.Natl. Acad. Sci. U.S.A., 89, pp.3414~3418 (1992)
- Markstein, M., "A regulatory code for neurogenic gene expression in the Drosophila embryo", Development, 131, pp.2387~2394 (2004)
- Zinzen, R. P., "Evolution of the ventral midline in insect embryos", Dev. Cell, 11, pp.895~902 (2006)
- Gray, S., "Short-range repression permits multiple enhancers to function autonomously within a complex promoter", Genes Dev., 8, pp.1829~1838 (1994)
- Gray, S., "Short-range transcriptional repressors mediate both quenching and direct repression within complex loci in Drosophila", Genes Dev., 10, pp.700~710 (1996)
- Joung-Woo Hong, "ZAS3 represses NFκB-dependent transcription by direct competition for DNA binding", BMB Reports, 43(12), pp.807~812 (2010), 생화학분자생물학회
- Yun Deng, "Synergistic efficacy of LBH and αB-crystallin through inhibiting transcriptional activities of p53 and p21", BMB Reports, 43(6), pp.432~437 (2010), 생화학분자생물학회
- Han, K., "Transcriptional repression by the Drosophila even-skipped protein: definition of a minimal repression domain", Genes Dev., 7, pp.491~503 (1993)
- Um, M., "The transcriptional repressor even-skipped interacts directly with TATA-binding protein", Mol. Cell. Biol., 15, pp.5007~5016 (1995)
- Drouin, J., "Novel glucocorticoid receptor complex with DNA element of the hormone-repressed POMC gene", EMBO J., 12, pp.145~156 (1993)
- Stathopoulos, A., "Whole-genome analysis of dorsal- ventral patterning in the Drosophila embryo", Cell, 111, pp.687~701 (2002)
- Jiang, J., "The dorsal morphogen gradient regulates the mesoderm determinant twist in early Drosophila embryos", Genes Dev., 5, pp.1881~1891 (1991)
- Campos-Ortega, J. A., "The embryonic development of Drosophila melanogaster" (1985), Springer-Verlag
- Morel, V., "Repression by suppressor of hairless and activation by Notch are required to define a single row of single-minded expressing cells in the Drosophila embryo", Genes Dev., 14, pp.377~388 (2000)