Adenovirus vector-mediated FAM176A overexpression induces cell death in human H1299 non-small cell lung cancer cells
0
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
FAM176A (family with sequence similarity 176 member A) is anovel molecule related to programmed cell death. A decreasedexpression of FAM176A has been found in several types ofhuman tumors in including lung cancers. In the present study,we investigated the biological activities of FAM176A on thehuman non–small cell lung cancer cell line H1299 cells. Weconstructed a recombinant adenovirus 5-FAM176A vector(Ad5-FAM176A) and evaluated the expression and anti-tumoractivities in vitro. Cell viability analysis revealed that theadenovirus-mediated increase of FAM176A inhibited thegrowth of the tumor cells in a dose- and time-dependentmanner. This inhibitory effect was mediated by both autophagyand apoptosis that involved caspase activation. In addition, cellcycle analysis suggested that Ad5-FAM176A could induce cellcycle arrest at the G2/M phase, all of which suggested thatadenovirus-mediated FAM176A gene transfer might present anew therapeutic approach for lung cancer treatment.
Abstract
FAM176A (family with sequence similarity 176 member A) is anovel molecule related to programmed cell death. A decreasedexpression of FAM176A has been found in several types ofhuman tumors in including lung cancers. In the present study,we investigated the biological activities of FAM176A on thehuman non–small cell lung cancer cell line H1299 cells. Weconstructed a recombinant adenovirus 5-FAM176A vector(Ad5-FAM176A) and evaluated the expression and anti-tumoractivities in vitro. Cell viability analysis revealed that theadenovirus-mediated increase of FAM176A inhibited thegrowth of the tumor cells in a dose- and time-dependentmanner. This inhibitory effect was mediated by both autophagyand apoptosis that involved caspase activation. In addition, cellcycle analysis suggested that Ad5-FAM176A could induce cellcycle arrest at the G2/M phase, all of which suggested thatadenovirus-mediated FAM176A gene transfer might present anew therapeutic approach for lung cancer treatment.
참고문헌 (16)
- Parkin, D. M., "Global cancer statistics, 2002", CA Cancer J. Clin, 55, pp.74~108 (2002)
- Jemal, A., "Global patterns of cancer incidence and mortality rates and trends", Cancer Epidemiol. Biomarkers Prev, 19, pp.1893~1907 (2010)
- Pirozynski, M., "RETRACTED: 100 years of lung cancer", Respir. Med, 100, pp.2073~2084 (2006)
- Wakelee, H. A., "Lung cancer incidence in never smokers", J. Clin. Oncol, 25, pp.472~478 (2007)
- Ramalingam, S. S., "Lung cancer: new biological insights and recent therapeutic advances", CA Cancer J. Clin, 61, pp.91~112 (2011)
- Wang, L., "TMEM166, a novel transmembrane protein, regulates cell autophagy and apoptosis", Apoptosis, 12, pp.1489~1502 (2007)
- Xu, D., "Expression of TMEM166 protein in human normal and tumor tissues", Appl. Immunohistochem. Mol. Morphol, 21, pp.543~552 (2013)
- Sun, W., "Transmembrane protein 166 expression in esophageal squamous cell carcinoma in xinjiang, China", Asian Pac. J. Cancer Prev, 13, pp.3713~3716 (2012)
- Chang, Y., "Adenovirus vector-mediated expression of TMEM166 inhibits human cancer cell growth by autophagy and apoptosis in vitro and in vivo", Cancer Lett, 328, pp.126~134 (2013)
- Sherr, C. J., "The RB and p53 pathways in cancer", Cancer Cell, 2, pp.103~112 (2002)
- Roth, J. A., "Adenovirus p53 gene therapy", Expert. Opin. Biol. Ther, 6, pp.55~61 (2006)
- El-Deiry, W. S., "The role of p53 in chemosensitivity and radiosensitivity", Oncogene, 22, pp.7486~7495 (2003)
- Ma, J. -T., "Synergistic cytotoxic effects of recombinant human adenovirus p53 and radiation at various time points in A549 lung adenocarcinoma cells", Oncol. Lett, 4, pp.529~533 (2012)
- Peng, Z., "Current status of gendicine in China: recombinant human Ad-p53 agent for treatment of cancers", Hum. Gene. Ther, 16, pp.1016~1027 (2005)
- Eisenberg-Lerner, A., "Life and death partners: apoptosis, autophagy and the cross-talk between them", Cell Death. Differ, 16, pp.966~975 (2009)
- Żuryń, A, "Expression of cyclin B1 after induction of senescence and cell death in non-small cell lung carcinoma A549 cells", Folia. Histochem. Cytobiol, 50, pp.58~67 (2012)