Zinc finger and BTB domain-containing protein 3 is essential for the growth of cancer cells
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ZBTB3 belongs to the Zinc finger and BTB/POZ domaincontaining transcription factor family; however, its biologicalrole has rarely been studied. We demonstrate for the first time,to our knowledge, that ZBTB3 is an essential factor for cancercell growth via the regulation of the ROS detoxificationpathway. Suppression of ZBTB3 using two different shorthairpin RNAs in human melanoma, lung carcinoma, and breastcarcinoma results in diminished cell growth. In addition, wefound that suppression of ZBTB3 activates a caspase cascade,including caspase-9, -3, and PARP leading to cellular apoptosis,resulting from failed ROS detoxification. We identified thatZBTB3 plays an important role in the gene expression of ROSdetoxification enzymes. Our results reveal that ZBTB3 mayplay a critical role in cancer cell growth via the ROSdetoxification system. Therefore, therapeutic strategies thattarget ZBTB3 could be used in selective cancer treatments.
Abstract
ZBTB3 belongs to the Zinc finger and BTB/POZ domaincontaining transcription factor family; however, its biologicalrole has rarely been studied. We demonstrate for the first time,to our knowledge, that ZBTB3 is an essential factor for cancercell growth via the regulation of the ROS detoxificationpathway. Suppression of ZBTB3 using two different shorthairpin RNAs in human melanoma, lung carcinoma, and breastcarcinoma results in diminished cell growth. In addition, wefound that suppression of ZBTB3 activates a caspase cascade,including caspase-9, -3, and PARP leading to cellular apoptosis,resulting from failed ROS detoxification. We identified thatZBTB3 plays an important role in the gene expression of ROSdetoxification enzymes. Our results reveal that ZBTB3 mayplay a critical role in cancer cell growth via the ROSdetoxification system. Therefore, therapeutic strategies thattarget ZBTB3 could be used in selective cancer treatments.
참고문헌 (23)
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