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YAC tripeptide of epidermal growth factor promotes the proliferation of HaCaT keratinocytes through activation of EGFR

김유리|김민서|이경진|한장희|박경한|유연호
생화학분자생물학회 (Korean Society Of Biochemistry And Molecular Biology)|2014.10|BMB Reports|제47권 제10호|pp. 581-586

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참고문헌

키워드

EGFSkin regenerationEGFRKeratinocyte proliferationTripeptide
주제분류화학

초록

Epidermal growth factor (EGF) is known to play key roles inskin regeneration and wound-healing. Here, we demonstratethat Pep2-YAC, a tripeptide covering residues 29-31 in the Bloop of EGF, promotes the proliferation of HaCaT keratinocyteswith activity comparable to EGF. The treatment of HaCaT cellswith Pep2-YAC induced phosphorylation, internalization, anddegradation of EGFR and organization of signaling complexes,which consist of Grb2, Gab1, SHP2, and PI3K. In addition, itstimulated the phosphorylation of ERK1/2 at Thr 202/Tyr 204and of Akt1 at Ser 473 and the nuclear translocation of EGFR,STAT3, c-Jun, and c-Fos. These results suggest that Pep2-YACmay be useful as a therapeutic agent for skin regeneration andwound-healing as an EGFR agonist.

Abstract

Epidermal growth factor (EGF) is known to play key roles inskin regeneration and wound-healing. Here, we demonstratethat Pep2-YAC, a tripeptide covering residues 29-31 in the Bloop of EGF, promotes the proliferation of HaCaT keratinocyteswith activity comparable to EGF. The treatment of HaCaT cellswith Pep2-YAC induced phosphorylation, internalization, anddegradation of EGFR and organization of signaling complexes,which consist of Grb2, Gab1, SHP2, and PI3K. In addition, itstimulated the phosphorylation of ERK1/2 at Thr 202/Tyr 204and of Akt1 at Ser 473 and the nuclear translocation of EGFR,STAT3, c-Jun, and c-Fos. These results suggest that Pep2-YACmay be useful as a therapeutic agent for skin regeneration andwound-healing as an EGFR agonist.

참고문헌 (26)

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