Characterization of the cellular localization of C4orf34 as a novel endoplasmic reticulum resident protein
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Human genome projects have enabled whole genomemapping and improved our understanding of the genes inhumans. However, many unknown genes remain to befunctionally characterized. In this study, we characterizedhuman chromosome 4 open reading frame 34 gene(hC4orf34). hC4orf34 was highly conserved from invertebrateto mammalian cells and ubiquitously expressed in the organsof mice, including the heart and brain. Interestingly, hC4orf34is a novel ER-resident, type I transmembrane protein. Mutantanalysis showed that the transmembrane domain (TMD) ofhC4orf34 was involved in ER retention. Overall, our resultsindicate that hC4orf34 is an ER-resident type I transmembraneprotein, and might play a role in ER functions including Ca2+homeostasis and ER stress.
Abstract
Human genome projects have enabled whole genomemapping and improved our understanding of the genes inhumans. However, many unknown genes remain to befunctionally characterized. In this study, we characterizedhuman chromosome 4 open reading frame 34 gene(hC4orf34). hC4orf34 was highly conserved from invertebrateto mammalian cells and ubiquitously expressed in the organsof mice, including the heart and brain. Interestingly, hC4orf34is a novel ER-resident, type I transmembrane protein. Mutantanalysis showed that the transmembrane domain (TMD) ofhC4orf34 was involved in ER retention. Overall, our resultsindicate that hC4orf34 is an ER-resident type I transmembraneprotein, and might play a role in ER functions including Ca2+homeostasis and ER stress.
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