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Stanniocalcin 2 enhances mesenchymal stem cell survival by suppressing oxidative stress

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초록

To overcome the disadvantages of stem cell-based cell therapy like low cell survival at the disease site, we used stanniocalcin 2 (STC2), a family of secreted glycoprotein hormones that function to inhibit apoptosis and oxidative damage and to induce proliferation. STC2 gene was transfected into two kinds of stem cells to prolong cell survival and protect the cells from the damage by oxidative stress. The stem cells expressing STC2 exhibited increased cell viability and improved cell survival as well as elevated expression of the pluripotency and self-renewal markers (Oct4 and Nanog) under sub-lethal oxidative conditions. Up-regulation of CDK2 and CDK4 and down-regulation of cell cycle inhibitors p16 and p21 were observed after the delivery of STC2. Furthermore, STC2 transduction activated pAKT and pERK 1/2 signal pathways. Taken together, the STC2 can be used to enhance cell survival and maintain long-term stemness in therapeutic use of stem cells.

Abstract

To overcome the disadvantages of stem cell-based cell therapy like low cell survival at the disease site, we used stanniocalcin 2 (STC2), a family of secreted glycoprotein hormones that function to inhibit apoptosis and oxidative damage and to induce proliferation. STC2 gene was transfected into two kinds of stem cells to prolong cell survival and protect the cells from the damage by oxidative stress. The stem cells expressing STC2 exhibited increased cell viability and improved cell survival as well as elevated expression of the pluripotency and self-renewal markers (Oct4 and Nanog) under sub-lethal oxidative conditions. Up-regulation of CDK2 and CDK4 and down-regulation of cell cycle inhibitors p16 and p21 were observed after the delivery of STC2. Furthermore, STC2 transduction activated pAKT and pERK 1/2 signal pathways. Taken together, the STC2 can be used to enhance cell survival and maintain long-term stemness in therapeutic use of stem cells.

참고문헌 (38)

  1. Mimeault M, "Stem cells: a revolution in therapeutics-recent advances in stem cell biology and their therapeutic applications in regenerative medicine and cancer therapies", Clin Pharmacol Ther, 82, pp.252~264 (2007)
  2. Estrada JC, "Human mesenchymal stem cell-replicative senescence and oxidative stress are closely linked to aneuploidy", Cell Death Dis, 4, pp.e691~ (2013)
  3. Sart S, "Preconditioning stem cells for in vivo delivery", Biores Open Access, 3, pp.137~149 (2014)
  4. Salem HK, "Mesenchymal stromal cells: current understanding and clinical status", Stem Cells, 28, pp.585~596 (2010)
  5. Sotiropoulou PA, "Characterization of the optimal culture conditions for clinical scale production of human mesenchymal stem cells", Stem Cells, 24, pp.462~471 (2006)
  6. Bonab MM, "Aging of mesenchymal stem cell in vitro", BMC Cell Biol, 7, pp.14~ (2006)
  7. Bahmani B, "Lipocalin 2 decreases senescence of bone marrow-derived mesenchymal stem cells under sub-lethal doses of oxidative stress", Cell Stress Chaperones, 19, pp.685~693 (2014)
  8. Sakata H, "Neural stem cells genetically modified to overexpress cu", zn-superoxide dismutase enhance amelioration of ischemic stroke in mice, 2012, pp.43, 2423~2429
  9. Yeung BH, "Evolution and roles of stanniocalcin", Mol Cell Endocrinol, 349, pp.272~280 (2012)
  10. Wagner GF, "The stanniocalcin family of proteins", J Exp Zool A Comp Exp Biol, 305, pp.769~780 (2006)
  11. Chang AC, "Mammalian stanniocalcins and cancer", Endocr Relat Cancer, 10, pp.359~373 (2003)
  12. Tamura S, "Clinical significance of STC1 gene expression in patients with colorectal cancer", Anticancer Res, 31, pp.325~329 (2011)
  13. Fujiwara Y, "Assessment of Stanniocalcin-1 mRNA as a molecular marker for micrometastases of various human cancers", Int J Oncol, 16, pp.799~804 (2000)
  14. Kim SJ, "Stanniocalcin-1 protects retinal ganglion cells by inhibiting apoptosis and oxidative damage", PLoS One, 8, pp.e63749~ (2013)
  15. Li-ming Wu, "Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage", Journal of Veterinary Science, 15(4), pp.475~483 (2014), 대한수의학회[DOI]
  16. Wu S, "Stanniocalcin 1 acts as a paracrine regulator of growth plate chondrogenesis", J Biol Chem, 281, pp.5120~5127 (2006)
  17. Westberg JA, "Hypoxic preconditioning induces neuroprotective stanniocalcin-1 in brain via IL-6 signaling", Stroke, 38, pp.1025~1030 (2007)
  18. Levy O, "mRNA-engineered mesenchymal stem cells for targeted delivery of interleukin-10to sites of inflammation", Blood, 122, pp.23~32 (2013)
  19. Baghbaderani BA, "A review of bioreactor protocols for human neural precursor cell expansion in preparation for clinical trials", Curr Stem Cell Res Ther, 6, pp.229~254 (2011)
  20. Wang K, "Redox homeostasis: the linchpin in stem cell self-renewal and differentiation", Cell Death Dis, 4, pp.e537~ (2013)
  21. Das B, "HIF-2alpha suppresses p53 to enhance the stemness and regenerative potential of human embryonic stem cells", Stem Cells, 30, pp.1685~1695 (2012)
  22. Liu TC, "Encapsulation of cardiac stem cells in superoxide dismutase-loaded alginate prevents doxorubicin-mediated toxicity", J Tissue Eng Regen Med, 7, pp.302~311 (2013)
  23. Zhong J, "Hydrogel matrix to support stem cell survival after brain transplantation in stroke", Neurorehabil Neural Repair, 24, pp.636~644 (2010)
  24. Kim PH, "Injectable multifunctional microgel encapsulating outgrowth endothelial cells and growth factors for enhanced neovascularization", J Control Release, 187, pp.1~13 (2014)
  25. Okabe H, "Genome-wide analysis of gene expression in human hepatocellular carcinomas using cDNA microarray: identification of genes involved in viral carcinogenesis and tumor progression", Cancer Res, 61, pp.2129~2137 (2001)
  26. Bouras T, "Stanniocalcin 2is an estrogen-responsive gene coexpressed with the estrogen receptor in human breast cancer", Cancer Res, 62, pp.1289~1295 (2002)
  27. Haixiao Wang, "STC2 is upregulated in hepatocellular carcinoma and promotes cell proliferation and migration in vitro", BMB Reports, 45(11), pp.629~634 (2012), 생화학분자생물학회
  28. de Almeida PE, "Immunogenicity of pluripotent stem cells and their derivatives", Circ Res, 112, pp.549~561 (2013)
  29. Le Blanc K, "Mesenchymal stromal cells: Tissue repair and immune modulation", Cytotherapy, 8, pp.559~561 (2006)
  30. Hsiao ST, "Ischemic preconditioning for cell-based therapy and tissue engineering", Pharmacol Ther, 142, pp.141~153 (2014)
  31. Satyanarayana A, "Mammalian cell-cycle regulation:several Cdks, numerous cyclins and diverse compensatory mechanisms", Oncogene, 28, pp.2925~2939 (2009)
  32. Hydbring P, "Cdk2 : a key regulator of the senescence control function of Myc", Aging (Albany NY), 2, pp.244~250 (2010)
  33. Gogolin S, "CDK4 inhibition restores G(1)-S arrest in MYCN-amplified neuroblastoma cells in the context of doxorubicin-induced DNA damage", Cell Cycle, 12, pp.1091~1104 (2013)
  34. Romanov VS, "Cyclin-dependent kinase inhibitor p21(Waf1): contemporary view on its role in senescence and oncogenesis", Biochemistry (Mosc), 77, pp.575~584 (2012)
  35. Sheppard KE, "The cell-cycle regulator CDK4: an emerging therapeutic target in melanoma", Clin Cancer Res, 19, pp.5320~5328 (2013)
  36. Tsai CC, "Oct4and Nanog directly regulate Dnmt1 to maintain self-renewal and undifferentiated state in mesenchymal stem cells", Mol Cell, 47, pp.169~182 (2012)
  37. Baer PC, "Human adipose-derived mesenchymal stem cells in vitro: evaluation of an optimal expansion medium preserving stemness", Cytotherapy, 12, pp.96~106 (2010)
  38. Go MJ, "Forced expression of Sox2 or Nanog in human bone marrow derived mesenchymal stem cells maintains their expansion and differentiation capabilities", Exp Cell Res, 314, pp.1147~1154 (2008)

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