Methyltransferase and demethylase profiling studies during brown adipocyte differentiation
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Although brown adipose tissue is important with regard to energy balance, the molecular mechanism of brown adipocyte differentiation has not been extensively studied. Specifically, regulation factors at the level of protein modification are largely unknown. In this study, we examine the changes in the expression level of enzymes which are involved in protein lysine methylation during brown adipocyte differentiation. Several enzymes, in this case SUV420H2, PRDM9, MLL3 and JHDM1D, were found to be up-regulated. On the other hand, Set7/9 was significantly down-regulated. In the case of SUV420H2, the expression level increased sharply during brown adipocyte differentiation, whereas the expression of SUV420H2 was marginally enhanced during the white adipocyte differentiation. The knock-down of SUV420H2 caused the suppression of brown adipocyte differentiation, as compared to a scrambled control. These results suggest that SUV420H2, a methyltransferase, is involved in brown adipocyte differentiation, and that the methylation of protein lysine is important in brown adipocyte differentiation.
Abstract
Although brown adipose tissue is important with regard to energy balance, the molecular mechanism of brown adipocyte differentiation has not been extensively studied. Specifically, regulation factors at the level of protein modification are largely unknown. In this study, we examine the changes in the expression level of enzymes which are involved in protein lysine methylation during brown adipocyte differentiation. Several enzymes, in this case SUV420H2, PRDM9, MLL3 and JHDM1D, were found to be up-regulated. On the other hand, Set7/9 was significantly down-regulated. In the case of SUV420H2, the expression level increased sharply during brown adipocyte differentiation, whereas the expression of SUV420H2 was marginally enhanced during the white adipocyte differentiation. The knock-down of SUV420H2 caused the suppression of brown adipocyte differentiation, as compared to a scrambled control. These results suggest that SUV420H2, a methyltransferase, is involved in brown adipocyte differentiation, and that the methylation of protein lysine is important in brown adipocyte differentiation.
참고문헌 (31)
- Park A, "Distinction of white, beige and brown adipocytes derived from mesenchymal stem cells", World J Stem Cells, 6, pp.33~42 (2014)
- Harms M, "Brown, beige fat:development, function and therapeutic potential", Nature Med, 19, pp.1252~1263 (2013)
- Yao X, "Recent progress in the study of brown adipose tissue", Cell Biosci, 1, pp.35~ (2011)
- 배광희, "Involvement of protein tyrosine phosphatases in adipogenesis: New anti-obesity targets?", BMB Reports, 45(12), pp.700~706 (2012), 생화학분자생물학회
- Whetstine JR, "Methylation : a multifaceted modification – looking at transcription and beyond", Biochim Biophys Acta, 1839, pp.1351~1352 (2014)
- Biggar KK, "Non-histone protein methylation as a regulator of cellular signalling and function", Nature Rev Mol Cell Biol, 16, pp.5~17 (2015)
- Hamamoto R, "Critical roles of non-histone protein lysine methylation in human tumorigenesis", Nature Rev Cancer, 15, pp.110~124 (2015)
- Zhang X, "Emerging roles of lysine methylation on non-histone proteins", Cell Mol Life Sci, 72, pp.4257~4272 (2015)
- Okamura M, "Role of histone methylation and demethylation in adipogenesis and obesity", Organogenesis, 6, pp.24~32 (2010)
- Ohno H, "EHMT1 controls brown adipose cell fate and thermogenesis through the PRDM16 complex", Nature, 504, pp.163~167 (2013)
- Del Rizzo PA, "Molecular basis for substrate recognition by lysine methyltransferases and demethylases", Biochim Biophys Acta, 1839, pp.1404~1415 (2014)
- Souza PP, "The histone methyltransferase SUV420H2, heterochromatin proteins HP1 interact but show different dynamic behaviours", BMC Cell Biol, 20, pp.41~ (2009)
- Schotta G, "A chromatin-wide transition to H4K20 monomethylation impairs genome integrity, programmed DNA rearrangements in the mouse", Genes Dev, 22, pp.2048~2061 (2008)
- Shinchi Y, "SUV420H2suppresses breast cancer cell invasion through down regulation of the SH2 domain-containing focal adhesion protein tensin-3", Exp Cell Res, 334, pp.90~99 (2015)
- Tachibana M, "Set domain-containing protein, G9a, is a novel lysine-preferring mammalian histone methyltransferase with hyperactivity and specific selectivity to lysines 9, 27 of histone H3", J Biol Chem, 276, pp.25309~25317 (2001)
- Barski A, "High-resolution profiling of histone methylations in the human genome", Cell, 129, pp.823~837 (2007)
- Huang C, "Dual-specificity histone demethylase KIAA1718 (KDM7A) regulates neuronal differentiation through FGF4", Cell Res, 20, pp.154~165 (2010)
- Tsukada Y, "KDM7 is a dual demethylase for histone H3 Lys 9, Lys 27, functions in brain development", Genes Dev, 24, pp.432~437 (2010)
- Osawa T, "Increased expression of histone demethylase JHDM1D under nutrient starvation suppresses tumor growth via downregulating angiogenesis", Proc Natl Acad Sci U S A, 108, pp.20725~20729 (2011)
- Yokoyama A, "KIAA1718 is a histone demethylase that erases repressive histone methyl marks", Gene Cells, 15, pp.867~873 (2010)
- Hess JL, "Mechanisms of transformation by MLL", Crit Rev Eukaryot Gene Exp, 14, pp.235~254 (2004)
- Dreijerink KM, "The multiple endocrine neoplasia type 1(MEN1)tumor suppressor regulates peroxisome proliferator-activated receptor gamma-dependent adipocyte differentiation", Mol Cell Biol, 29, pp.5060~5069 (2009)
- Lee J, "Targeted inactivation of MLL3 histone H3-Lys-4 methyltransferase activity in the mouse reveals vital roles for MLL3 in adipogenesis", Proc Natl Acad Sci, 105, pp.19229~19234 (2008)
- Seale P, "Transcriptional control of brown fat determination by PRDM16", Cell Metab, 6, pp.38~54 (2007)
- Kim WK, "MAP kinase phosphatase 3 inhibits brown adipocyte differentiation via regulation of ERK phosphorylation", Mol Cell Endocrinol, 416, pp.70~76 (2015)
- Kamal AH, "Investigation of adipocyte proteome during the differentiation of brown preadipocytes", J Proteomics, 94, pp.327~336 (2013)
- Lee DS, "c-Jun regulates adipocyte differentiation via the KLF15-mediated mode", Biochem Biophys Res Comm, 469, pp.552~558 (2016)
- 최혜령, "Protein tyrosine phosphatase profiling studies during brown adipogenic differentiation of mouse primary brown preadipocytes", BMB Reports, 46(11), pp.539~543 (2013), 생화학분자생물학회
- Kim EY, "Selection of aptamers for mature white adipocytes by Cell SELEX using flow cytometry", PLoS ONE, 9, pp.e97747~ (2014)
- 김효정, "Caffeine inhibits adipogenesis through modulation of mitotic clonal expansion and the AKT", GSK3 pathway in 3T3-L1 adipocytes, 2016, pp.49(2), 111~115[DOI]
- Lifang Hu, "Knockdown of microtubule actin crosslinking factor 1 inhibits cell proliferation in MC3T3-E1 osteoblastic cells", BMB Reports, 48(10), pp.583~588 (2015), 생화학분자생물학회