Human adipose-derived mesenchymal stem cell spheroids improve recovery in a mouse model of elastase-induced emphysema
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Emphysema, a pathologic component of the chronic obstructive pulmonary disease, causes irreversible destruction of lung. Many researchers have reported that mesenchymal stem cells can regenerate lung tissue after emphysema. We evaluated if spheroid human adipose-derived mesenchymal stem cells (ASCs) showed greater regenerative effects than dissociated ASCs in mice with elastase-induced emphysema. ASCs were administered via an intrapleural route. Mice injected with spheroid ASCs showed improved regeneration of lung tissues, increased expression of growth factors such as fibroblast growth factor-2 (FGF2) and hepatocyte growth factor (HGF), and a reduction in proteases with an induction of protease inhibitors when compared with mice injected with dissociated ASCs. Our findings indicate that spheroid ASCs show better regeneration of lung tissues than dissociated ACSs in mice with elastase-induced emphysema.
Abstract
Emphysema, a pathologic component of the chronic obstructive pulmonary disease, causes irreversible destruction of lung. Many researchers have reported that mesenchymal stem cells can regenerate lung tissue after emphysema. We evaluated if spheroid human adipose-derived mesenchymal stem cells (ASCs) showed greater regenerative effects than dissociated ASCs in mice with elastase-induced emphysema. ASCs were administered via an intrapleural route. Mice injected with spheroid ASCs showed improved regeneration of lung tissues, increased expression of growth factors such as fibroblast growth factor-2 (FGF2) and hepatocyte growth factor (HGF), and a reduction in proteases with an induction of protease inhibitors when compared with mice injected with dissociated ASCs. Our findings indicate that spheroid ASCs show better regeneration of lung tissues than dissociated ACSs in mice with elastase-induced emphysema.
참고문헌 (34)
- Lange P, "Prediction of the clinical course of the chronic obstructive pulmonary disease, using the new GOLD classification: a study of the general population", Am J Respir Crit Care Med, 186, pp.975~981 (2012)
- Wang Y, "Effects of mesenchymal stem cells on matrix metalloproteinase synthesis in cardiac fibroblasts", Exp Biol Med (Maywood), 236, pp.1197~1204 (2011)
- Churg A, "Series "matrix metalloproteinases in lung health and disease": Matrix metalloproteinases in COPD", Eur Respir J, 39, pp.197~209 (2012)
- Park IS, "A novel threedimensional adipose-derived stem cell cluster for vascular regeneration in ischemic tissue", Cytotherapy, 16, pp.508~522 (2014)
- Zhao YF, "Mesenchymal stem cell-based developmental endothelial locus-1 gene therapy for acute lung injury induced by lipopolysaccharide in mice", Mol Med Rep, 9, pp.1583~1589 (2014)
- Guan XJ, "Mesenchymal stem cells protect cigarette smoke-damaged lung and pulmonary function partly via VEGF-VEGF receptors", J Cell Biochem, 114, pp.323~335 (2013)
- Rojas M, "Bone marrowderived mesenchymal stem cells in repair of the injured lung", Am J Respir Cell Mol Biol, 33, pp.145~152 (2005)
- 김유선, "Tracking Intravenous Adipose-Derived Mesenchymal Stem Cells in a Model of Elastase-Induced Emphysema", Tuberculosis and Respiratory Diseases, 77(3), pp.116~123 (2014), 대한결핵및호흡기학회
- Terrovitis J, "Noninvasive quantification and optimization of acute cell retention by in vivo positron emission tomography after intramyocardial cardiac-derived stem cell delivery", J Am Coll Cardiol, 54, pp.1619~1626 (2009)
- Zhang M, "Cardiomyocyte grafting for cardiac repair: graft cell death and anti-death strategies", J Mol Cell Cardiol, 33, pp.907~921 (2001)
- Yoon HH, "Enhanced cartilage formation via three-dimensional cell engineering of human adipose-derived stem cells", Tissue Eng Part A, 18, pp.1949~1956 (2012)
- Cheng NC, "The influence of spheroid formation of human adipose-derived stem cells on chitosan films on stemness and differentiation capabilities", Biomaterials, 33, pp.1748~1758 (2012)
- Bartosh TJ, "Aggregation of human mesenchymal stromal cells (MSCs) into 3D spheroids enhances their anti-inflammatory properties", Proc Natl Acad Sci U S A, 107, pp.13724~13729 (2010)
- Okumura K, "Salivary gland progenitor cells induced by duct ligation differentiate into hepatic and pancreatic lineages", Hepatology, 38, pp.104~113 (2003)
- Bates RC, "Apoptosis induced by inhibition of intercellular contact", J Cell Biol, 125, pp.403~415 (1994)
- Yamaguchi Y, "Mesenchymal stem cell spheroids exhibit enhanced in-vitro and in-vivo osteoregenerative potential", BMC Biotechnol, 14, pp.105~ (2014)
- Park IS, "Enhanced angiogenic effect of adipose-derived stromal cell spheroid with low-level light therapy in hind limb ischemia mice", Biomaterials, 35, pp.9280~9289 (2014)
- Ware JA, "Angiogenesis in ischemic heart disease", Nat Med, 3, pp.158~164 (1997)
- Yanagisawamiwa A, "Salvage of Infarcted Myocardium by Angiogenic Action of Basic Fibroblast Growth-Factor", Science, 257, pp.1401~1403 (1992)
- Scholz D, "Arteriogenesis, a new concept of vascular adaptation in occlusive disease", Angiogenesis, 4, pp.247~257 (2001)
- Shigemura N, "Amelioration of pulmonary emphysema by in vivo gene transfection with hepatocyte growth factor in rats", Circulation, 111, pp.1407~1414 (2005)
- Zeng X, "VEGF enhances pulmonary vasculogenesis and disrupts lung morphogenesis in vivo", Dev Dyn, 211, pp.215~227 (1998)
- Kanazawa H, "Role of vascular endothelial growth factor in the pathogenesis of the chronic obstructive pulmonary disease", Med Sci Monit, 13, pp.Ra189~Ra195 (2007)
- Zhang S, "The effects of the spheroid formation of adiposederived stem cells in a microgravity bioreactor on stemness properties and therapeutic potential", Biomaterials, 41, pp.15~25 (2015)
- Wang H, "Vascular endothelial growth factor upregulates the expression of matrix metalloproteinases in vascular smooth muscle cells - Role of flt-1", Circ Res, 83, pp.832~840 (1998)
- Wang H, "Hepatocyte growth factor enhances MMP activity in human endothelial cells", Biochem Biophys Res Commun, 272, pp.900~905 (2000)
- Seandel M, "Growth factor-induced angiogenesis in vivo requires specific cleavage of fibrillar type I collagen", Blood, 97, pp.2323~2332 (2001)
- Loffek S, "Series "matrix metalloproteinases in lung health and disease": Biological role of matrix metalloproteinases: a critical balance", Eur Respir J, 38, pp.191~208 (2011)
- Shipley JM, "Metalloelastase is required for macrophage-mediated proteolysis and matrix invasion in mice", Proc Natl Acad Sci U S A, 93, pp.3942~3946 (1996)
- Churg A, "Mechanisms of cigarette smoke-induced COPD: insights from animal models", Am J Physiol Lung Cell Mol Physiol, 294, pp.L612~L631 (2008)
- Churg A, "Effect of an MMP-9", MMP-12 inhibitor on smoke-induced emphysema and airway remodeling in guinea pigs, 2007, pp.62, 706~713
- Visse R, "Matrix metalloproteinases and tissue inhibitors of metalloproteinases: structure, function, and biochemistry", Circ Res, 92, pp.827~839 (2003)
- Lozito TP, "Mesenchymal stem cells inhibit both endogenous and exogenous MMPs via secreted TIMPs", J Cell Physiol, 226, pp.385~396 (2011)
- Gruber R, "Bone marrow stromal cells can provide a local environment that favors migration and formation of tubular structures of endothelial cells", Tissue Eng, 11, pp.896~903 (2005)
