본문 바로가기
KERD
논문KCI 등재

Protective effects of Tat-DJ-1 protein against streptozotocin-induced diabetes in a mice model

1

조회

0

다운로드

5

인용

0

참고문헌

키워드

주제분류화학

초록

A major feature of type 1 diabetes mellitus (T1DM) is hyperglycemia and dysfunction of pancreatic -cells. In a previous study, we have shown that Tat-DJ-1 protein inhibits pancreatic RINm5F -cell death caused by oxidative stress. In this study, we examined effects of Tat-DJ-1 protein on streptozotocin (STZ)-induced diabetic mice. Wild type (WT) Tat-DJ-1 protein transduced into pancreas where it markedly inhibited pancreatic -cell destruction and regulated levels of serum parameters including insulin, alkaline phosphatase (ALP), and free fatty acid (FFA) secretion. In addition, transduced WT Tat-DJ-1 protein significantly inhibited the activation of NF-B and MAPK (ERK and p38) expression as well as expression of COX-2 and iNOS in STZ exposed pancreas. In contrast, treatment with C106A mutant Tat-DJ-1 protein showed no protective effects. Collectively, our results indicate that WT Tat-DJ-1 protein can significantly ameliorate pancreatic tissues in STZ-induced diabetes in mice.

Abstract

A major feature of type 1 diabetes mellitus (T1DM) is hyperglycemia and dysfunction of pancreatic -cells. In a previous study, we have shown that Tat-DJ-1 protein inhibits pancreatic RINm5F -cell death caused by oxidative stress. In this study, we examined effects of Tat-DJ-1 protein on streptozotocin (STZ)-induced diabetic mice. Wild type (WT) Tat-DJ-1 protein transduced into pancreas where it markedly inhibited pancreatic -cell destruction and regulated levels of serum parameters including insulin, alkaline phosphatase (ALP), and free fatty acid (FFA) secretion. In addition, transduced WT Tat-DJ-1 protein significantly inhibited the activation of NF-B and MAPK (ERK and p38) expression as well as expression of COX-2 and iNOS in STZ exposed pancreas. In contrast, treatment with C106A mutant Tat-DJ-1 protein showed no protective effects. Collectively, our results indicate that WT Tat-DJ-1 protein can significantly ameliorate pancreatic tissues in STZ-induced diabetes in mice.

참고문헌 (50)

  1. Lukic ML, "New insight into early events in type 1 diabetes: role for islet stem cell exosomes", Diabetes, 63, pp.835~837 (2014)[DOI]
  2. Daneman D, "Type 1 diabetes", Lancet, 367, pp.847~858 (2006)[DOI]
  3. Welters A, "Diabetes Mellitus;Metabolism of human diseases: organ physiology and pathophysiology", pp.163~173 (2014), Springer
  4. Rui L, "Energy metabolism in the liver", Compr Physiol, 4, pp.177~197 (2014)
  5. Fiorentino TV, "Hyperglycemia-induced oxidative stress and its role in diabetes mellitus related cardiovascular diseases", Curr Pharm Des, 19, pp.5695~5703 (2013)[DOI]
  6. Esposito K, "Stress hyperglycemia, inflammation, and cardiovascular events", Diabetes Care, 26, pp.1650~1651 (2003)
  7. Hayashi K, "Strain differences in the diabetogenic activity of streptozotocin in mice", Biol Pharm Bull, 29, pp.1110~1119 (2006)[DOI]
  8. Mikio I, "Characterization of low dose streptozotocin-induced progressive diabetes in mice", Environ Toxicol Pharmacol, 9, pp.71~78 (2001)[DOI]
  9. Nagakubo D, "DJ-1, a novel oncogene which transforms mouse NIH3T3 cells in cooperation with ras", Biochem Biophys Res Commun, 231, pp.509~513 (1997)[DOI]
  10. Wilson MA, "The role of cysteine oxidation in DJ-1 function and dysfunction", Antioxid Redox Signal, 15, pp.111~122 (2011)[DOI]
  11. Ariga H, "Neuroprotective function of DJ-1 in Parkinson's disease", Oxidative Med Cell Longev, 2013, pp.683920~ (2013)
  12. Bonifati V, "Mutations in the DJ-1 gene associated with autosomal recessive early-onset parkinsonism", Science, 299, pp.256~259 (2003)[DOI]
  13. Jain D, "Age- and diet-dependent requirement of DJ-1 for glucose homeostasis in mice with implications for human type 2 diabetes", J Mol Cell Biol, 4, pp.221~230 (2012)[DOI]
  14. Yu HH, "Stable overexpression of DJ-1 protects H9c2 cells against oxidative stress under a hypoxia condition", Cell Biochem Funct, 31, pp.643~651 (2013)[DOI]
  15. Inden M, "PARK7 DJ-1 protects against degeneration of nigral dopaminergic neurons in Parkinson's disease rat model", Neurobiol Dis, 24, pp.144~158 (2006)[DOI]
  16. Kim RH, "Hypersensitive of DJ-1-deficient mice to 1-methtyl-4-phenyl- 1,2,3,6-tetrahydropyrindine (MPTP) and oxidative stress", Proc Natl Acad Sci U S A, 102, pp.5215~5220 (2005)[DOI]
  17. Wadia JS, "Protein transduction technology", Curr Opin Biotechnol, 13, pp.52~56 (2002)[DOI]
  18. van den Berg A, "Protein transduction domain delivery of therapeutic macromolecules", Curr Opin Biotechnol, 22, pp.888~893 (2011)[DOI]
  19. Kubo E, "TAT-mediated PRDX6 protein transduction protects against eye lens epithelial cell death and delays lens opacity", Am J Physiol Cell Physiol, 294, pp.C842~C855 (2008)[DOI]
  20. Embury J, "Proteins linked to a protein transduction domain efficiently transduce pancreatic islets", Diabetes, 50, pp.1706~1713 (2001)[DOI]
  21. 조효상, "Effects of PEP-1-FK506BP on cyst formation in polycystic kidney disease", BMB Reports, 50(9), pp.460~465 (2017), 생화학분자생물학회
  22. 조효상, "Transduced Tat-DJ-1 protein inhibits cytokines-induced pancreatic RINm5F cell death", BMB Reports, 49(5), pp.297~302 (2016), 생화학분자생물학회
  23. Jo HS, "Tat-DJ-1 inhibits oxidative stress-mediated RINm5F cell death through suppression of <TEX>$NF-{\kappa}B$<", TEX> and MAPK activation, 2016, pp.25, 2589~2598
  24. Jo HS, "Tat-DJ-1 enhances cell survival by inhibition of oxidative stress, <TEX>$NF-{\kappa}B$<", TEX> and MAPK activation in HepG2 cells, 2017, pp.39, 511~521
  25. Kim MJ, "Transduced Tat-glyoxalase protein attenuates streptozotocin-induced diabetes in a mouse model", Biochem Biophys Res Commun, 430, pp.294~300 (2013)[DOI]
  26. Szkudelski T, "The mechanisms of alloxan and streptozotocin action in <TEX>${\beta}$<", TEX>-cells of rat pancreas, 2001, pp.50, 536~546
  27. Jain D, "DJ-1 protects pancreatic beta cells from cytokine- and streptozotocinmediated cell death", PLoS One, 10, pp.e0138535~ (2015)[DOI]
  28. Robertson RP, "Beta-cell deterioration during diabetes: what's in the gun?", Trends Endocrinol Metab, 20, pp.388~393 (2009)[DOI]
  29. Mota M, "Molecular mechanism of lipotoxicity and glucotoxicity in nonalcoholic fatty liver disease", Metabolism, 65, pp.1049~1061 (2016)[DOI]
  30. Navarro C, "Free radicals scavenger and antihepatotoxic activity of Rosmarinus", Planta Med, 59, pp.312~314 (1993)[DOI]
  31. Singh R, "Antioxidant and toxicological evaluation of Cassia sopherain streptozotocin-induced diabetic Wister rats", Pharmacognosy Res, 5, pp.225~232 (2013)[DOI]
  32. Ren H, "DJ-1, a cancer and Parkinson's disease associated protein, regulates autophagy through JNK pathway in cancer cells", Cancer Lett, 297, pp.101~108 (2010)[DOI]
  33. Gu L, "Involvement of ERK1", 2 signaling pathway in DJ-1-induced neuroprotection against oxidative stress, 2009, pp.383, 469~474
  34. Im JY, "DJ-1 protects against oxidative damage by regulating the thioredoxin", ASK1 complex, 2010, pp.67, 203~208
  35. Jaramillo-Gomez J, "Overexpression of DJ-1 protects against C2-ceramide-induced neuronal death through activation of the PI3K", AKT pathway and inhibition of autophagy, 2015, pp.603, 71~76
  36. Sakai N, "p38 MAPK phosphorylation and NF-kappa B activation in human crescentic glomerulonephritis", Nephrol Dial Transplant, 17, pp.998~1004 (2002)[DOI]
  37. Shi Y, "Protective and anti-angiopathy effects of ginsenoside Re against diabetes mellitus via the activation of p38MAPK, ERK1", 2 and JNK signaling, 2016, pp.14, 4849~4856
  38. Zhang Y, "Lentinan protects pancreatic <TEX>${\beta}$<", TEX> cells from STZ-induced damage, 2016, pp.20, 1803~1812
  39. Sakai N, "Involvement of extracellular signal-regulated kinase and p38 in human diabetic nephropathy", Am J Kidney Dis, 45, pp.54~65 (2005)[DOI]
  40. Weih F, "Multiorgan inflammation and hematopoietic abnormalities in mice with a targeted disruption of RelB, a member of the <TEX>$NF-{\beta}B$<", TEX>, Cell, pp.1995, 80[DOI]
  41. Mollah ZH, "Abnormal NF-kappa B function characterizes human type 1 diabetes dendritic cells and monocytes", J Immunol, 180, pp.3166~3175 (2008)[DOI]
  42. Lundh M, "Small-molecule inhibition of inflammatory beta cell death", Diabetes Obes Metab, 15, pp.176~184 (2013)[DOI]
  43. Donath MY, "Type 2 diabetes as an inflammatory disease", Nat Rev Immunol, 11, pp.98~107 (2011)[DOI]
  44. Rabinovitch A, "Cytokines and their roles in pancreatic islet beta-cell destruction and insulin-dependent diabetes mellitus", Biochem Pharmacol, 55, pp.1139~1149 (1998)[DOI]
  45. Kellogg AP, "Cyclooxygenase-2 pathway as a potential therapeutic target in diabetic peripheral neuropathy", Curr Drug Targets, 9, pp.68~76 (2008)[DOI]
  46. Bujalska-Zadrozny M, "Influence of nitric oxide synthase or cyclooxygenase inhibitors on cannabinosids activity in streptozotocininduced neuropathy", Pharmacol Rep, 67, pp.209~216 (2015)[DOI]
  47. Bujalska M, "Effects of cyclooxygenase and nitric oxide synthase inhibitors on streptozotocin-induced hyperalgesia in rats", Pharmacol, 81, pp.151~157 (2008)[DOI]
  48. Ahn EH, "Tat-ATOX1 inhibits streptozotocin-induced cell death in pancreatic RINm5F cells and attenuates diabetes in a mouse model", Int J Mol Med, 38, pp.217~224 (2016)[DOI]
  49. 양승주, "Anti-inflammatory and anti-oxidative effects of 3-(naphthalen-2-yl(propoxy)methyl)azetidine hydrochloride on β-amyloid-induced microglial activation", BMB Reports, 50(12), pp.634~639 (2017), 생화학분자생물학회
  50. 안수연, "Celastrol suppresses expression of adhesion molecules and chemokines by inhibiting JNK-STAT1", NF-κB activation in poly(I:C)-stimulated astrocytes, 2017, pp.50(1), 25~30[DOI]

관련 논문AI추천

Transduced Tat-DJ-1 protein inhibits cytokines-induced pancreatic RINm5F cell death김상진, 박정환, 여은지, 음원식, 이치헌, 조수빈, 조효상, 차현주, 최수영, 최연주, Hyeon Ji Yeo생화학분자생물학회 · BMB Reports2016 · 제49권 제5호Tat-CIAPIN1 protein prevents against cytokine-induced cytotoxicity in pancreatic RINm5F β-cells권혁일, 여현지, 음원식, Dae Won Kim, Min Jea Shin, Soo Young Choi생화학분자생물학회 · BMB Reports2021 · 제54권 제9호Crocin Improves Oxidative Stress by Potentiating Intrinsic Anti-Oxidant Defense Systems in Pancreatic Cells During Uncontrolled HyperglycemiaAli Noroozadeh, Amirhossein Sahebkar, Habib Yaribeygi, Mohammad Taghi Mohammadi, Thomas P. Johnston대한약침학회 · Journal of Pharmacopuncture2019 · 제22권 제2호PEP-1-paraoxonase 1 fusion protein prevents cytokine-induced cell destruction and impaired insulin secretion in rat insulinoma cells강형경, 권혁일, 박진서, 음원식, 이수진, 최수영, 최연주생화학분자생물학회 · BMB Reports2018 · 제51권 제10호Protective effects of PEP-1-Catalase on stress-induced cellular toxicity and MPTP-induced Parkinson’s disease권혁일, 김대원, 김덕수, 박진서, 손은정, 신민재, 안은희, 엄선애, 음원식, 전수정, 정석영, 조성우, 조효상, 최수영, 한규형생화학분자생물학회 · BMB Reports2015 · 제48권 제7호Protective effects of Tat-NQO1 against oxidative stress-induced HT-22 cell damage, and ischemic injury in animals김대원, 김덕수, 박정환, 박진서, 신민재, 안은희, 여은지, 음원식, 이치헌, 정석영, 조성우, 조수빈, 조효상, 최수영, 최연주, 한규형, Hyeon Ji Yeo생화학분자생물학회 · BMB Reports2016 · 제49권 제11호

이 간행물의 다른 논문 (8건)