본문 바로가기
KERD
논문KCI 등재

Improved human hematopoietic reconstitution in HepaRG co-transplanted humanized NSG mice

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류화학

초록

Several humanized mouse models are being used to study humanspecific immune responses and diseases. However, the pivotal needs of fetal tissues for the humanized mice model have been huddled because of the demand for ethical and medical approval. Thus, we have verified the hematopoietic and immunomodulatory function of HepaRG and developed a new and easy humanized mouse model to replace the use of fetal liver tissue. HepaRG co-transplanted Hu-NSG mice significantly increased CD45+ lymphocytes and CD19+ B cells and CD3+ T cells than normal Hu-NSG, suggesting enhanced reconstitution of the human immune system. These results have improved the applicability of humanized mice by developing new models easily accessible.

Abstract

Several humanized mouse models are being used to study humanspecific immune responses and diseases. However, the pivotal needs of fetal tissues for the humanized mice model have been huddled because of the demand for ethical and medical approval. Thus, we have verified the hematopoietic and immunomodulatory function of HepaRG and developed a new and easy humanized mouse model to replace the use of fetal liver tissue. HepaRG co-transplanted Hu-NSG mice significantly increased CD45+ lymphocytes and CD19+ B cells and CD3+ T cells than normal Hu-NSG, suggesting enhanced reconstitution of the human immune system. These results have improved the applicability of humanized mice by developing new models easily accessible.

참고문헌 (26)

  1. Lan P, "Reconstitution of a functional human immune system in immunodeficient mice through combined human fetal thymus", liver and CD34+ cell transplantation, 2006, pp.108, 487~492
  2. Lavender KJ, "BLThumanized C57BL", 6 Rag2-, -CD47-, pp.-mice are resistant to GVHD and develop B-and T-cell immunity to HIV infection, Blood[DOI]
  3. Rongvaux A, "Development and function of human innate immune cells in a humanized mouse model", Nat Biotechnol, 32, pp.364~ (2014)
  4. Saito Y, "Peripheral blood CD34+ cells efficiently engraft human cytokine knock-in mice", Blood, 128, pp.1829~1833 (2016)
  5. Shultz LD, "Generation of functional human T-cell subsets with HLA-restricted immune responses in HLA class I expressing NOD", SCID, Proc Natl Acad Sci U S A, pp.2010, 107[DOI]
  6. Klein F, "HIV therapy by a combination of broadly neutralizing antibodies in humanized mice", Nature, 492, pp.118~ (2012)
  7. Yajima M, "A new humanized mouse model of Epstein-Barr virus infection that reproduces persistent infection, lymphoproliferative disorder, and cell-mediated and humoral immune responses", J Infect Dis, 198, pp.673~682 (2008)
  8. Lan P, "Reconstitution of a functional human immune system in immunodeficient mice through combined human fetal thymus", liver and CD34+ cell transplantation, 2006, pp.108, 487~492
  9. Fomin ME, "Human fetal liver cultures support multiple cell lineages that can engraft immunodeficient mice", Open Biol, 7, pp.170108~ (2017)
  10. Guo J, "Humanized mice reveal an essential role for human hepatocytes in the development of the liver immune system", Cell Death Dis, 9, pp.667~ (2018)
  11. Touraine JL, "Bone-Marrow and Fetal-Liver Transplantation in Immunodeficiencies and Inborn Errors of Metabolism : Lack of Significant Restriction of T-Cell Function in Long-Term Chimeras Despite HLA-Mismatch", Immunol Rev, 71, pp.103~121 (1983)
  12. Kapp MB, "Ethical and legal issues in research involving human subjects: do you want a piece of me?", J Clin Pathol, 59, pp.335~339 (2006)
  13. Geraghty R, "Guidelines for the use of cell lines in biomedical research", Br J Cancer, 111, pp.1021~ (2014)
  14. Kubo T, "Maintenance of hepatic progenitor-like characteristics of HepaRG cells by cultivation on VECELL Inserts", ALTEX, 21, pp.71~79 (2016)
  15. Parent R, "Origin and characterization of a human bipotent liver progenitor cell line", Gastroenterology, 126, pp.1147~1156 (2004)
  16. Cerec V, "Transdifferentiation of hepatocyte-like cells from the human hepatoma HepaRG cell line through bipotent progenitor", Hepatology, 45, pp.957~967 (2007)
  17. Mou F, "The Mst1 and Mst2kinases control activation of rho family GTPases and thymic egress of mature thymocytes", J Exp Med, 209, pp.741~759 (2012)
  18. Clambey ET, "Hypoxia-inducible factor-1 alpha-dependent induction of FoxP3 drives regulatory T-cell abundance and function during inflammatory hypoxia of the mucosa", Proc Natl Acad Sci U S A, 109, pp.E2784~2793 (2012)
  19. Qian Y, "IL-17 signaling in host defense and inflammatory diseases", Cell Mol Immunol, 7, pp.328~333 (2010)
  20. Tascher G, "In-Depth Proteome Analysis Highlights HepaRG Cells as a Versatile Cell System Surrogate for Primary Human Hepatocytes", Cells, 8, pp.192~ (2019)
  21. Jabari S, "Cellular plasticity of trans-and dedifferentiation markers in human hepatoma cells in vitro and in vivo", Int J Oncol, 35, pp.69~80 (2009)
  22. Lu M, "Enhanced generation of hematopoietic cells from human hepatocarcinoma cell-stimulated human embryonic and induced pluripotent stem cells", Exp Hematol, 37, pp.924~936 (2009)
  23. Rangaraju S, "Differential Phagocytic Properties of CD45(low)Microglia and CD45(high)Brain Mononuclear Phagocytes-Activation and Age-Related Effects", Front Immunol, 9, pp.405~ (2018)
  24. Higuchi Y, "The human hepatic cell line HepaRG as a possible cell source for the generation of humanized liver TK-NOG mice", Xenobiotica, 44, pp.146~153 (2014)
  25. Audige A, "Long-term leukocyte reconstitution in NSG mice transplanted with human cord blood hematopoietic stem and progenitor cells", BMC Immunol, 18, pp.28~ (2017)
  26. Ichii M, "Stromal cell-free conditions favorable for human B lymphopoiesis in culture", J Immunol Methods, 359, pp.47~55 (2010)

관련 논문AI추천

T Lymphocyte Development and Activation in Humanized Mouse Model이동율, 이지윤, 한아름한국발생생물학회 · 발생과 생식2019 · 제23권 제2호Scalable production of tissue-like vascularized liver organoids from human PSCsAlmaas Runar, Andersen Elisabeth, Åsrud Kathrine S., Baumgarten Saphira, Bezrouk Ales, Bogen Inger Lise, Boger Ida C., Bonanini Flavio, Bravo-Pérez Carlos, Chollet Maria Eugenia, Cizkova Dana, Corral Javier, Dejneka Alexandr, de la Morena-Barrio María Eugenia, Harrison Sean P., Kempf Henning, Kurek Dorota, Lunov Oleg, Mathapati Santosh, Melum Espen, Mokry Jaroslav, Mowinckel Marie-Christine, Nyman Tuula A., Park In-Hyun, Patterson Benjamin, Sandset Per Morten, Sharma Kulbhushan, Siller Richard, Skottvoll Frøydis S., Stavik Benedicte, Sullivan Gareth J., Tanaka Yoshiaki, Wilson Steven R., Wu Jun Jie, Xiang Yangfei, Zweigerdt Robert생화학분자생물학회 · Experimental and Molecular Medicine2023 · 제55권 Regulated differentiation of stem cells into an artificial 3D liver as a transplantable sourceFeng Chen, Hua Wang, Jia Xiao대한간학회 · Clinical and Molecular Hepatology2020 · 제26권 제2호Liver organoids: Current advances and future applications for hepatologyDongho Choi, Meritxell Huch, Michael Adisasmita, Michael Girma Mamo, Minseok Kang, Yohan Kim대한간학회 · Clinical and Molecular Hepatology2025 · 제31권 제2호A decade of liver organoids: Advances in disease modelingChun-Fang Yang, Jian-Ying Sheng, Junjun Ding, Yue Liu, Yun-Shen Chan대한간학회 · Clinical and Molecular Hepatology2023 · 제29권 제3호Severe combined immunodeficiency pig as an emerging animal model for human diseases and regenerative medicines김진회, 최영석, 홍권호, Muhammad Arsalan Iqbal생화학분자생물학회 · BMB Reports2019 · 제52권 제11호

이 간행물의 다른 논문 (6건)