Expression of Kip-related protein 4 gene (KRP4) in response to auxin and cytokinin during growth of Arabidopsis thaliana
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The cell cycle is regulated by cyclin-dependent kinase (CDK)-cyclin complexes as well as other regulators. We isolated Kip-related protein 4 (KRP4) cDNA that encodes 289 amino acids including six conserved domains. To investigate the expression pattern of KRP4 as well as of other cell cycle-related genes associated with plant hormones, Arabidopsis seedlings were cultured on MS medium containing auxin or cytokinin. All seedlings treated with phytohormones displayed an increased proportion of cells in S phase. A higher proportion of cells in G2 phase was observed in seedlings treated with NAA. RT-PCR confirmed that the expression of KRP4 was decreased after treatment with phytohormones, and that CDKA and D-type cyclin transcription was increased. Additionally, mitotic cyclins were up-regulated by NAA treatment. These results suggest that KRP4 as well as other cell cycle-related genes might contribute to the control of plant growth in response to exogenous hormones.
Abstract
The cell cycle is regulated by cyclin-dependent kinase (CDK)-cyclin complexes as well as other regulators. We isolated Kip-related protein 4 (KRP4) cDNA that encodes 289 amino acids including six conserved domains. To investigate the expression pattern of KRP4 as well as of other cell cycle-related genes associated with plant hormones, Arabidopsis seedlings were cultured on MS medium containing auxin or cytokinin. All seedlings treated with phytohormones displayed an increased proportion of cells in S phase. A higher proportion of cells in G2 phase was observed in seedlings treated with NAA. RT-PCR confirmed that the expression of KRP4 was decreased after treatment with phytohormones, and that CDKA and D-type cyclin transcription was increased. Additionally, mitotic cyclins were up-regulated by NAA treatment. These results suggest that KRP4 as well as other cell cycle-related genes might contribute to the control of plant growth in response to exogenous hormones.
참고문헌 (21)
- Pavletich, N. P., "Mechanisms of cyclin-dependent kinase regulation: structures of Cdks, their cyclin activators, and Cip and INK4 inhibitors", J. Mol. Biol., 287, pp.821~828 (1999)
- Toyoshima, H., "p27, a novel inhibitor of G1 cyclin-Cdk protein kinase activity, is related to p21", Cell, 78, pp.67~74 (1994)
- De Veylder, L., "Functional analysis of cyclin-dependent kinase inhibitors of Arabidopsis", Plant Cell, 13, pp.1653~1667 (2001)
- Wang, H., "A plant cyclin-dependent kinase inhibitor gene", Nat, 386, pp.451~452 (1997)
- Sherr, C. J., "CDK inhibitors: positive and negative regulators of G1-phase progression", Genes Dev., 13, pp.1501~1512 (1999)
- Oakenfull, E. A., "Plant D-type cyclins and the control of G1 progression", Philos. Trans. R. Soc. Lond. B. Biol. Sci., 357, pp.749~760 (2002)
- Woodward, A. W., "Auxin: Regulation, Action, and Interaction", Ann. Bot., 95, pp.707~735 (2005)
- Jacobs, T., "Why do plant cells divide", Plant Cell, 9, pp.1021~1029 (1997)
- Zhang, K., "Cytokinin controls the cell cycles at mitosis by stimulating the tyrosine dephosphorylation and activation of p34cdc2-like H1 histone kinase", Planta, 200, pp.2~12 (1996)
- Carle, S. A., "Hormonal control of gene expression during reactivation of the cell cycle in tobacco mesophyll protoplasts", Plant Growth Regul., 17, pp.221~230 (1998)
- Kapros, T., "Differential expression of histone H3 gene variants during cell cycle and somatic embryogenesis in alfalfa", Plant. Physiol., 98, pp.621~625 (1992)
- Soni, R., "A family of cyclin D homologs from plants differentially controlled by growth regulators and containing the conserved retinoblastoma protein interaction motif", Plant Cell, 7, pp.85~103 (1995)
- Harashima, H., "Auxin is required for the assembly of A-type cyclin-dependent kinase complexes in tobacco cell suspension culture", J. Plant. Physiol., 164, pp.1103~1112 (2007)
- Dewitte, W., "Arabidopsis CYCD3 D-type cyclins link cell proliferation and endocycles and are rate-limiting for cytokinin responses", PNAS., 104, pp.14537~14542 (2007)
- Riou-Khamlichi, C., "Cytokinin activation of Arabidopsis cell division through a D-type cyclin", Science, 283, pp.1541~1544 (1999)
- Richard, C., "Effect of auxin, cytokinin, and sucrose on cell cycle gene expression in Arabidopsis thaliana cell suspension cultures", Plant. Cell, 69, pp.167~176 (2002)
- Zhou, Y., "The plant cyclin-dependent kinase inhibitor ICK1 has distinct function", Plant Cell Rep., 20, pp.967~975 (2002)
- Ormenese, S., "Analysis of the spatial expression pattern of seven kip related proteins (KRPs) in the shoot apex of Arabidopsis thaliana", Ann. Bot., 93, pp.575~580 (2004)
- Himanen, K., "Auxin-mediated cell cycle activation during early lateral root initiation", Plant Cell, 14, pp.2339~2351 (2002)
- Thompson, J., "CLUSTAL X: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positionspecific gap penalties and weight matrix choice", Nucleic Acids Res., 22, pp.4673~4680 (1994)
- Felsenstein, J., "PHYLIP (phylogeny inference package). Version 3.2", Cladistics, 5, pp.164~166 (1989)